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Showing 1 to 2 of 2 for “"split-TEV"”.

  1. Nucleic Acid Scaffold-dependent Proximity-mediated Enzyme Response (NASPER) - A Proof of Concept Study

    … this study are the HIV protease (HIVPR) and the split-TEV protease, which require dimerisation/fragment complementation for catalytic activity. In Chapter 3, the two designed PUF proteins were first purified and tested in vitro using fluorescence polarisation experiments to assess RNA binding. …

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  2. Understanding the role of the Notch intracellular domain in transcription hub formation

    … recruited at a target locus, the Enhancer of split-Complex (E(spl)-C), but appeared less robustly enriched over background than Su(H) and Mastermind. When NICD dimerization was perturbed, lower levels of Su(H) were recruited. In contrast, deleting the C-terminus of NICD had little effect on …

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