Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"splicing regulation"”.
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CELF1, PTBP1, and RBFOX2-mediated alternative splicing regulation in cardiovascular diseases
Alternative splicing (AS) is dysregulated in Type 1 diabetic (T1D) and hypoplastic left heart syndrome (HLHS) patient hearts but the mechanisms responsible are unclear. Here, we provide evidence that in these patient’s hearts that dysregulation of the RNA binding proteins (RBPs) CELF1, PTBP1, and …
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Incomplete paralog compensation and splicing regulation by TRA2A and TRA2B in cancer
… explores the functional relationship between the splicing factor paralogs TRA2A and TRA2B, members of the serine/arginine-rich (SR) protein family. In a subset of cancer cell lines, TRA2A emerges as a selective dependency due to incomplete compensation by TRA2B, revealing a synthetic lethal …
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A Genome-Scale Analysis of Alternative Splicing Regulation by Splicing Factors in C. elegans
Alternative splicing (AS) is a key mechanism that contributes to the high level of complexity present within metazoan proteomes. This process is highly regulated and important for the development for any animal. With advances in high-throughput technologies, our knowledge of the many AS events that …
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Molecular Mechanisms of Alternative Splicing Regulation: An Investigation of the Spliceosome Repressed by Hnrnp L on Cd45 Exon 4
Alternative splicing is a key step in gene regulation and involves the differential selection of splice sites to generate different pre-mRNA transcripts. It has been shown that 90-95% of pre-mRNAs are alternatively spliced in human cells. Pre-mRNA splicing is catalyzed the spliceosome, which …
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Multiple regulatory layers in the establishment of Rbfox2 splicing networks
Regulated alternative splicing is mediated by RNA binding proteins (RBPs) recognizing short sequence motifs in nascent transcripts. The Rbfox RBPs are highly conserved splicing factors that regulate tissue-specific exon inclusion by binding the RNA motif UGCAUG. We sought to comprehensively define …
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Mechanisms governing transposable element expression in the Drosophila germ line
… work has shown key differences between the regulation of the P-element and other transposons by the piRNA pathway. Notably, the dominant mechanism suppressing Pelement activity in germ cells is the regulation of its splicing through a currently unknown mechanism. This splicing regulation …
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Nova Regulation of Alternative Splicing in the CNS
… that Nova-1 acts as a regulator of alternative splicing in vivo. In addition to the known target Nova-dependent splicing regulation, exon 3A of glycine receptor α2 subunit (GlyRα2), two new targets are identified, which are the alternative exon, E9 in the inhibitory neurotransmitter receptor …
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Phosphorylation of The Smooth Muscle Master Splicing Regulator, RBPMS-A, Regulates its Splicing Activity
… RBPMS as a master regulator of alternative splicing in differentiated smooth muscle cells (SMCs), responsible for controlling numerous components critical to differentiated SMC function (Nakagaki-Silva et al., 2019). Therefore, the precise control of this splicing regulator is vital in …
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Exploring the functional conservation of muscleblind (Mbl) proteins
… aspects of RNA metabolism, including alternative splicing. Disruption of Muscleblind in several animals lends to a variety of defects and disease, including the multi-systemic disorder Myotonic Dystrophy (DM). Though much is known about the involvement of Muscleblind in DM, there is much basic …
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Isoform-Specific Expression During Embryo Development in Arabidopsis and Soybean
… (pre-mRNA) in a eukaryotic organism undergoes splicing, in some cases resulting in the formation of more than one splice variant, a process called alternative splicing. RNA-Seq provides a major opportunity to capture the state of the transcriptome, which includes the detection of alternative …
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Elucidating the structure-function relationship of the cell-type specific splicing regulator, RBPMS
… various aspects of RNA metabolism, including splicing. The RNA binding protein, RNA binding protein with multiple splicing (RBPMS), regulates the alternative splicing programme in differentiated vascular smooth muscle cells (VSMCs). RBPMS has an N-terminal RNA recognition motif (RRM) that …
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Interpretable Computational Modeling of pre-mRNA Splicing for Multiple Eukaryotic Species
… steps in eukaryotic gene expression is pre-mRNA splicing, whereby intronic sequences are excised from immature pre-mRNA transcripts and the remaining exonic sequences are joined together. This process is catalyzed by the spliceosome, a large complex of proteins and RNAs. A variety of RNA sequence …
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Quantitative modeling of 5' splice site subclass regulation and evolution
Pre-mRNA splicing is an essential molecular process required for eukaryotic gene expression. In this thesis, I present a previously unknown mechanism of splicing regulation where a family of splicing factors, the LUC7 family, compete to differentially impact 5→ splice site (5→ SS) selection in a …
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Alternative isoform regulation in myotonic dystrophy
… portions of RNA, which sequester or activate RNA splicing factor proteins, leading to widespread deleterious changes in transcriptome isoform usage. We developed a method for studying transcriptomes, RNAseq, which provides a high resolution, digital inventory of gene and isoform expression. By …
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Context-Dependent Function of the Splicing Factor hnRNP L
… impacted (~95% of humans genes), alternative splicing is one of the most extensively used mechanisms for generating proteomic diversity and cellular complexity. Splicing of pre-mRNAs is carried out by a highly specialized, RNA-based macromolecular enzyme known as the spliceosome. The …
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Formation of an Exon-Defined A Complex Spliceosome Intermediate Results in CD45 Exon Repression
Alternative splicing is a common means for genetic regulation in higher eukaryotes, and variability in splicing patterns results in a significant increase in protein diversity. Of particular interest is how the splicing machinery is regulated to allow for alternative splicing while maintaining …
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Genetic Interactions of Factors That Regulate Alternative Rna Splicing In The Male Germ Line of Drosophila
<p>Alternative RNA splicing is a critical process that contributes variety to protein functions, and further controls cell differentiation and normal development. Although it is known that most eukaryotic genes produce multiple transcripts in which splice site selection is regulated, how RNA …
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Assembling The Building Blocks For A Unified Splicing Code
… product through a process called alternative splicing. Alternative splicing is a widespread mechanism of tissue-specific regulation, and its misregulation has been implicated in diseases such as cancer. This motivates experimental and computational streams of research to understand splicing. …
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Regulation of Human Papillomavirus Type 16 Late L1 mRNA Splicing
… to cervical cancer. HPV-16 uses alternative splicing to regulate expression of early and late genes. HPV RNA elements and cellular factors control the expression level of viral proteins by regulating alternative splicing. This project was carried out to enhance our understanding of the …
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Splice Factor SFRS6: Regulation by p53 and Effect on IL-24 Splicing
<p>Alternative splicing of RNA transcripts is emerging as a major mechanism for expanding the proteome. Splice pattern changes are found in numerous diseases and cancers, indicating the importance of tight control over this process. While the mechanism of splicing has been described in detail, it …
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