Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 676 for “"splicing"”.
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A Genome-Scale Analysis of Alternative Splicing Regulation by Splicing Factors in C. elegans
Alternative splicing (AS) is a key mechanism that contributes to the high level of complexity present within metazoan proteomes. This process is highly regulated and important for the development for any animal. With advances in high-throughput technologies, our knowledge of the many AS events that …
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Phosphorylation of The Smooth Muscle Master Splicing Regulator, RBPMS-A, Regulates its Splicing Activity
… RBPMS as a master regulator of alternative splicing in differentiated smooth muscle cells (SMCs), responsible for controlling numerous components critical to differentiated SMC function (Nakagaki-Silva et al., 2019). Therefore, the precise control of this splicing regulator is vital in …
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Regulation of Human Telomerase Alternative Splicing
… is partially regulated at the level of RNA splicing, then telomerase activity may be modulated to increase the amount of nonfunctional transcripts and decrease the amount of full-length hTERT and this would be a novel anti-cancer therapeutic approach. A hTERT minigene was created to …
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In Vivo Studies of Yeast Mitochondrial Intron Splicing : Ectopic Branching and a Screen for Nuclear Encoded Splicing Factors
The splicing mechanism of group II introns is analogous to that of nuclear introns and it is generally thought that both share a common ancestor. This work contains two studies of group II intron splicing in yeast mitochondria. Previous studies done in collaboration with Dr. Anna Pyle at Yale …
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RNA sequencing for the study of splicing
… set of RNA molecules present in eukaryote cells, splicing emerges as the most prominent mechanism for message diversification. In recent years, applications of high throughput sequencing to RNA, known as RNA sequencing, have opened new avenues for the study of transcriptome composition, and have …
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Engineering RNA logic with synthetic splicing ribozymes
… In this thesis, I show the utility of splicing ribozymes for the biological engineer. Ribozymes allow the engineer to manipulate existing biological systems and to program self-modifying RNA systems. In addition, splicing ribozymes are easy to engineer, malleable, modular, and scalable. …
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The evolution of mRNA splicing in mammals
… I describe investigations into the evolution of splicing in mammals. I first investigate a small class of alternative splicing events, tandem splice sites, and show how they are used to introduce and remove coding sequence in a species-specific manner. I then describe the generation and analysis …
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The evolution and specificity of RNA splicing
… excise all the introns in a process known as splicing. This excision is determined by the sequence of the gene, but in a complicated way that is not fully understood. By analyzing gene sequences we can learn about how cells decide which sequences to splice. We have developed two new …
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Sapphire optical fibers: splicing and sensing applications
… sensor head. This thesis investigates possible splicing techniques to fabricate such a sensor set-up. Comparative results from experiments performed on splices that have been obtained by each of these techniques, are presented. Furthermore, two different sensor configurations using a sapphire …
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Expression profiling of second step splicing factors and role of PRP18a, a second step splicing factor in arabidopsis thaliana
Pre-mRNA splicing represents one of the most fundamental processes that is performed by snRNAs, proteins and a variety of non-snRNP proteins including PRP16, PRP17, PRP18, PRP22 and SLU7. In contrast to yeast and mammals, which contain single gene for each of their second step splicing factors, …
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Aberrant and Alternative Splicing of von Willebrand Factor
… are thought to disrupt the process of VWF splicing leading to VWD; however, this primarily acknowledges canonical splice site mutations, where the nucleotides are most integral and highly conserved to accomplish proper splicing. We hypothesized that pathologic splicing of VWF is likely an …
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Characterization of splicing mechanisms by single-molecule fluorescence
<p>Group II introns rank amongst the largest self-splicing ribozymes found in bacteria and organellar genomes of various eukaryotes. Despite the diversity in primary sequences, group II introns posses highly conserved secondary structures consisting of six domains (D1-D6). To perform its function, …
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iPLA2β, ALTERNATIVE SPLICING AND APOPTOSIS OF PANCREATIC ISLETS
… are bioactive lipids that can promote splicing of apoptosis-related genes, including caspase 9 and BCL-x. A recent study demonstrated that expression of neutral sphingomyelinase (NSMase), an enzyme that hydrolyzes sphingomyelins to generate ceramide, is regulated by Group VIA …
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Structure and Function Studies on tRNA Splicing Endonuclease
… essential processing step is performed by tRNA splicing endonuclease (TSEN), a highly conserved heterotetramer with two catalytic and two structural subunits. In addition to this conserved function, archaeal TSEN also processes pre-rRNA and yeast TSEN cleaves some mRNAs that encode mitochondrial …
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Investigation and Modelling of the Yarn Splicing Process
The pneumatic splicing technique was developed in the 1960's to overcome the deficiencies of knotting of textile yarns, but there has been little research into splicing because it is a complicated process, very difficult to observe. A splice is formed very quickly, using highly turbulent air within …
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Nova Regulation of Alternative Splicing in the CNS
… that Nova-1 acts as a regulator of alternative splicing in vivo. In addition to the known target Nova-dependent splicing regulation, exon 3A of glycine receptor α2 subunit (GlyRα2), two new targets are identified, which are the alternative exon, E9 in the inhibitory neurotransmitter receptor …
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On co-transcriptional splicing and U6 snRNA biogenesis
… and a poly(A) tail is added to its 3’end. Splicing, the process of intron removal, is carried out in the spliceosome, a megacomplex comprehending up to 300 proteins. The core components of the spliceosome that directly interact with the pre-mRNA are the small nuclear ribonucleoprotein …
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Latency, Expression and Splicing During Infection With HIV
… proviral latency and variation of expression and splicing in both the host and virus during infection. To evaluate the link between host chromatin and proviral latency, we compared genomic and epigenetic features to HIV-1 integration site data for latent and active provirus from five cell culture …
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