Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"spinning disk"”.
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Self-excited vibrations of a spinning disk
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 1995.
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Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy & Single Molecule Nano-Metronome & Single Molecule Study of Tetramethyrhodamine -Dna Interaction
The final part presents the single molecule study on a behavior of a popular fluorescent dye called Tetramethylrhodamine (TMR) attached to the end of DNA oligomer. The study reveals that in the absence of any biological activities of DNA, the fluorescence emission of TMR undergoes multiple slow …
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Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy &Amp; Single Molecule Nano-Metronome &Amp; Single Molecule Study of Tetramethyrhodamine-Dna Interaction
… lifetime data. The setup incorporates a spinning disk confocal unit to the conventional wide-field fluorescence lifetime imaging; therefore, it is a marriage between confocality and data acquisition speed. The detailed discussion about the principle of the setup in conjunction to the …
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The development of design criteria for a rotating disk containing noncentral circular hole patterns
… about circular, noncentral holes in a spinning disk is discussed. A theoretical approximation is first developed using the methods of elasticity and combining solutions to similar problems from Ling (reference 6) and Timoshenko and Goodier (reference 2). Results are then found in the …
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Generation of microbubble foam using a packed column
… the packed bed technique is superior to the spinning disk technique, the current microbubble foam generation method. This fact is partially due to the absence of an adequate large scale spinning disk device. In application, surfactant costs hamper the feasible use of a packed bed generation …
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In Vivo Imaging of Neutrophil Responses to Localized S. aureus Infection
… recruitment to a localized MRSA infection. Using spinning disk confocal microscopy, we developed a mouse model to visualize the behaviour of neutrophils in the skin following the introduction of an agarose bead embedded with GFP-expressing MRSA. We observed significant neutrophil recruitment not …
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Lab-bc: A Serverless Computing Platform for MIT Educators
… servers in our lab space, where despite frequent spinning disk failures and unreliable power delivery, the system provides high-performance, strongly-sandboxed Vivado instances to MIT students nationwide.
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Shedding light on the molecular interactomes of fast, complex biological processes using multispectral imaging with uncompromised spatiotemporal resolution
… integrated with multiple microscopes, such as a spinning disk confocal microscope and an oblique plane light sheet microscope. These instruments afforded continuous eight-channel timelapses in 2D using the multispectral spinning disk and eight-channel whole cell volumetric acquisition at four …
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Development of a centrifugal microfluidic device for separation and sorting in biological fluids
… tasks require precise control of the RPM and spinning time. Centrifugal micro-fluidic platforms have been successfully implemented for detection of hepatitis A, tetanus, as well as for measurement of haemoglobin and hematocrit, for DNA analysis, and for assessment of cardiac disease etc. by …
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Investigation of physical principles of protein phase transitions
… internal reflection fluorescence microscopy and spinning disk super resolution by optical pixel reassignment microscope. This reveals that the time-dependent changes in the physical and biochemical properties of biomolecular condensates are characterized by the coexistence of a growing solid …
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The Microbubble Assisted Bioremediation of Chlorinated Ethenes
… dioxide were effectively produced in a closed spinning disk generator, and were acceptable for delivering the gases to the columns. After the biodegradation study was completed, the microbubbles were found to have a pH of 4.4, due to the carbon dioxide. Microbubbles amended with NaOH to 0.01 …
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Practical Challenges in the Method of Controlled Lagrangians
… Lagrangian formulation. The first example is a spinning disk, a simplified, planar version of a spacecraft spin stabilization problem. The second example is a planar, streamlined underwater vehicle.
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Traps, Tensions and Transmissions: The Unionisation of Resistance Movements against External Destabilising Forces; Design, Development and Deployment of Holographic System for Active Surveillance and Targeted Manipulation
… imaging. This hybrid system is comprised of a spinning disk confocal unit, a spatial light modulator and a digital micromirror device, and is able to elucidate the dynamics of molecules, measure local forces, and re-localise or switch molecular behaviour. Here, we present the first application …
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Rotating Thin Films: the Vortex Fluidic Device
… were conducted using flat and hemispherical disks. In every spinning disk experiment the radial evolution of single droplets and the onset of instabilities, at the contact line and along the free surface of the film, were explored for a range of parameters. The parameters under investigation …
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Mitochondria-Endoplasmic Reticulum Contact Sites: Regulation and Roles in Coordinating Cell Fate Decisions
… receptor-3 (IP3R3). Using live cell spinning disk confocal microscopy, the loss of IP3R3 was demonstrated to impair ER to mitochondria Ca2+ transfer in these cancers, which subsequently inhibits apoptosis. This is proposed to occur through defective cristae remodelling and inefficient …
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DISSECTING THE ROLE OF REACTIVE OXYGEN SPECIES IN EARLY MAMMALIAN EMBRYOGENESIS
… a Nikon-Eclipse Ti2-E with Yokogawa W1- SoRa spinning disk confocal microscope equipped with a temperature-controlled CO2 chamber and appropriate lasers and filters at 30-second intervals. Our image analysis confirmed that HyPer7 successfully detects H2O2 in both oocytes and zygotes. However, …
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A metallomic, proteomic and lipidomic investigation of the malaria parasites digestive vacuole and insights into the mediators of haemozoin formation
… produced by the parasite. Live-cell imaging by spinning disk confocal microscopy revealed that neutral lipid bodies localised externally from the DV or in close contact with the DV membrane but were not found in the immediate vicinity of haemozoin. β-Haematin was found to occlude labelled …
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A spin on compressive sensing imaging : reticle-based single-pixel imaging system
… imaging (SPI) system are analyzed using a spinning reticle often referred to as a spinning coded aperture. The cost of imaging outside of the visual spectrum using FPA detectors becomes very expensive and an affordable alternative can have major implications in the commercial and military …
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Synthesis and characterization of tethered poly(n-isopropylacrylamide) for quantitative cell adhesion studies
… surfaces, both above and below the LCST using a spinning disc apparatus is also reported. This device applies a linear range of reproducible shear forces to cells attached at the interface of a single sample. In this work, a model marine bacterium, Cobetia marina, which adheres more readily to …
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Investigating the function of the hereditary spastic paraplegia protein spastin in the endomembrane system
Hereditary spastic paraplegias (HSPs) are genetically inherited neurological diseases characterised by the distal axonal degeneration of corticospinal neurons. Of the 80 genes currently associated with HSP, mutations in SPAST, encoding the protein spastin, are by far the most common cause of …