Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"spindle pole body"”.
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Investigation of coiled-coil interactions between proteins of the spindle pole body
The spindle pole body (SPB) is a large multi-protein complex that organizes microtubules in yeast. Through its function of nucleating and anchoring microtubules, the SPB is essential for cell viability. High-resolution structures of the SPB have not been achieved using x-ray crystallography, due to …
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Cell cycle control governing built-in asymmetry underlying the spindle pole body duplication cycle in Saccharomyces cerevisiae
… couple the polarised orientation of the mitotic spindle with an invariant age-dependent pattern of centrosome inheritance. Self- renewal supports tissue homeostasis by providing, in a balanced manner, one daughter cell retaining stemness and another daughter cell set to differentiate. Such …
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Using yeast to study neurodegenerative diseases : amyloid formation as a protective mechanism and a new Alzheimer's disease model
… resulted in the mislocalization of the core spindle pole body component Spc42 to the IPOD and an unduplicated spindle pole body. In mammalian cells aggresomes localize to centrosomes, the mammalian equivalent of the yeast spindle pole body. The finding that a yeast prion can interact with a …
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Regulation of the mitotic exit network components Tem1 and Cde 15 in Saccharomyces cerevisiae
… and sufficient for localization of CdclS5 to the spindle pole body, association with other Cdc 15 molecules, and a putative inhibitory domain are investigated. This work investigates the regulatory mechanisms controlling two essential components of the mitotic exit network.
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Regulation of mitotic spindle biogenesis in budding yeast
Assembly of the mitotic spindle is critically dependent on centrosome duplication (spindle pole body or SPB in yeast), separation of sister centrosomes and microtubule dynamics. Activation of Cdc28 (Cdk1) by tyrosine-19 dephosphorylation is essential for centrosome separation, the first step in …
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Myosin-Like Proteins in S. cerevisiae: Multifunctional, Structural Components of the Nuclear Envelope
… mRNA transport and processing machinery, and the spindle pole body (SPB; the yeast microtubule organizing center). Structural and microscopic analyses show that the Mlp proteins may form the nuclear basket in yeast, as well as interconnect NPCs into a network. Finally, a detailed functional study …
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Regulation of Anaphase Promoting Complex Coactivators
… replicated DNA; ~30% of these cells have bipolar spindles. Construction of bipolar spindles in these cells is strikingly sensitive to gene dosage of the stoichiometric Cdh1 inhibitor ACM1. CDH1-m11 cells with bipolar spindles fail to progress to anaphase, suggesting that Cdh1 inhibits multiple …
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Molecular Mechanisms Involved in Chromosome Segregation
… it to the microtubules (MTs) of the mitotic spindle. In human cells, the details of CCAN-CENP-ANuc assembly are unknown. This thesis investigates the structure and assembly of the human CCAN-CENP- ANuc complex, showing that CCAN tightly grips the linker DNA of the CENPANuc. CENP-C emerges as …