Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"spindle formation"”.
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Regulation of Mitotic Spindle Assembly in Caenorhabditis elegans Embryos
The mitotic spindle is a bipolar microtubule-based structure that mediates proper cell division by segregating the genetic material and by positioning the cytokinesis cleavage plane. Spindle assembly is a complex process, involving the modulation of microtubule dynamics, microtubule focusing at …
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Regulation of Chromosome Segregation by the Chromosomal Passenger Complex
… In eukaryotes, chromosome segregation depends on spindle formation. Chromosome-induced spindle assembly requires the chromosomal recruitment and activation of Aurora B. How this chromosome-activated kinase spatially disseminates signals that lead to spindle formation remains unclear. In the second …
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Mouse Oocyte Maturation and Embryo Development After Exposure to Vemurafenib (PLX4032), an Anti-Melanoma B-RAF V600E Inhibitor
… Oocytes were evaluated by cell cycle morphology, spindle formation and chromosomal alignment by im munofluorescence (IF) staining as well as DNA integrity by Terminal Transferase dUTP Nick End Labeling. Embryo development was evaluated by morphological changes and the C -R af bioactivity via a p-M …
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Control of Chromosome Segregation by the Aurora B Complex
… segregation during cell division requires spindle assembly around M-phase chromatin. In cells lacking centrosomes, such as those found in female meiosis, chromosomes themselves nucleate and stabilize microtubules in order to promote accurate spindle formation. Here we present a description …
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Tumor Suppressor function of the deubiquitinating enzyme BAP1 and its substrate gamma-tubulin In regulation of cell cycle and genome stability
… during mitosis with the help of the mitotic spindle. Thus, mitotic spindle formation is vital for proper chromosome congression, alignment and segregation during cell division. Dysregulation of any of the components involved in this process may cause mis-segregation of the chromosomes and …
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Centrosomin Self-Assembly and Centrosomal Protein Recruitment
… in animal cells, are important for mitotic spindle formation. Normally, each cell has two centrosomes which migrate to opposite sides of the nuclear envelope prior to entry into mitosis. Centrosomin (Cnn) is a major centrosomal protein that is important for nucleation and organization of …
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Regulation of mitotic spindle biogenesis in budding yeast
Assembly of the mitotic spindle is critically dependent on centrosome duplication (spindle pole body or SPB in yeast), separation of sister centrosomes and microtubule dynamics. Activation of Cdc28 (Cdk1) by tyrosine-19 dephosphorylation is essential for centrosome separation, the first step in …
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Cell Cycle Control by Cyclin-CDKS in Chlamydomonas Reinhardtii
… in cell division timing, DNA replication, spindle formation, and cytokinesis and explore the nature of their regulatory interactions and their control by the APC. We also describe a genetic screen to identify parallel pathways that promote cell cycle initiation alongside CDKA1 and speculate …
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Identification of Small Molecule Inhibitors Targeting Truncated Adenomatous Polyposis Coli (APC) as a Novel Therapeutic Strategy in Colorectal Cancer
… organization of actin and microtubule networks, spindle formation and chromosome segregation. Mutations in APC gene generate truncated gene products, leading to deregulation of these processes. Accumulating evidence suggest that these C terminally truncated APC may have gain of function …
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Identification of Small Molecule Inhibitors Targeting Truncated Adenomatous Polyposis Coli (APC) as a Novel Therapeutic Strategy in Colorectal Cancer
… organization of actin and microtubule networks, spindle formation and chromosome segregation. Mutations in APC gene generate truncated gene products, leading to deregulation of these processes. Accumulating evidence suggest that these C terminally truncated APC may have gain of function …
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SMARCA4/BRG1-Inactivating Mutations as Potential Predictive Markers for Aurora Kinase A-Targeted Therapy in NSCLCs
… AURKA-centered, centrosome-independent, mitotic spindle assembly machinery becomes solely responsible for mitotic spindle formation and proper chromosome segregation during mitosis. DLG7, the only known protein specific to this centrosome-independent mitotic spindle assembly, is required for the …
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Using Microscopy to Examine Mechanisms of Cell Division
… the assembly of a microtubule-based mitotic spindle. In the research presented here, I examined two aspects of mitotic spindle morphogenesis. First, I looked at how chromosomes attach to microtubules in mitosis. The capture of microtubule plus-ends by kinetochores (the site of …
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Transient CaMK-II Activation is Necessary for Left-Right Asymmetry in the Zebrafish Embryo
… (mediated through synapsin I) and to bipolar spindle formation in mammalian cells, among other cellular functions. In mammals, CaMK-II is encoded by four genes: α, β, δ, and γ. In zebrafish, like other teleost fish, gene duplication has yielded eight genes (α, αKAP, β1, β2, δ1, δ2, γ1, and …
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Embryo and Gamete Development Upon Exposure to CCA Components: CrO3, CuO, and AS2O5
… were analyzed for cell cycle progression. Spindle formation and chromosomal patterns in MI and MII oocytes were analyzed by immunofluorescent staining. A sperm motility index was used to evaluate sperm function in the presence of CCA components. Sperm viability was analyzed using …
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Regulation of MINK1 by APC, PKA and MARK3/4 and consequences for epithelial cell biology
… ERM proteins play an essential role in mitotic spindle formation, orientation and function and in cell migration. APC, too, has been linked to spindle orientation and MINK1 is phosphorylated during mitosis. Investigating the functional link suggested by these observations, I found that MINK1 …
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Regulation of γ-Tubulin Ring Complex Recruitment in Drosophila
… to the precise spatiotemporal regulation of spindle formation.
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Regulation of γ-Tubulin Ring Complex Recruitment in Drosophila
… to the precise spatiotemporal regulation of spindle formation.
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Analysis of the functions of phosphoprotein phosphatase 1 catalytic subunits during cell division
… be affecting processes required for the central spindle, whereas it contributes less to progressing cells through cell division in an earlier stage. Time-lapse imaging also revealed a few cells with defects in DNA segregation or central spindle formation when PP1β was degraded. Degradation of …
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The Role of LIM Kinase 1 and its Substrates in Cell Cycle Progression
… separation and proper assembly of bipolar spindles. Phosphorylated LIMK1 is recruited to the centrosomes during early prophase, where it colocalizes with ?-tubulin. Here, we report a novel functional cooperativity between Aur-A and LIMK1 through mutual phosphorylation. LIMK1 is recruited to …
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Characterization of a Novel Chromatin-Induced Mechanism that Couples Microtubule Disassembly and Nuclear Re-Formation
<p>Upon completion of mitosis, the disassembly of spindle components and reassembly of nuclear structures occur simultaneously around chromatin. Previous studies have suggested that an important step in this process is the inactivation of the Aurora B kinase by the Triple A-ATPase Cdc48/p97, which …
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