Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 71 for “"spindle assembly"”.
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… cells employ a surveillance mechanism termed Spindle assembly checkpoint that senses the lack of tension/attachment on the kinetochores and consequently blocks anaphase onset by inhibiting an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… cells employ a surveillance mechanism termed Spindle assembly checkpoint that senses the lack of tension/attachment on the kinetochores and consequently blocks anaphase onset by inhibiting an E3 ubiquitin ligase called anaphase-promoting complex. The roles of two kinases- BubR1 and Mps1, …
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Regulation of Mitotic Spindle Assembly in Caenorhabditis elegans Embryos
The mitotic spindle is a bipolar microtubule-based structure that mediates proper cell division by segregating the genetic material and by positioning the cytokinesis cleavage plane. Spindle assembly is a complex process, involving the modulation of microtubule dynamics, microtubule focusing at …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… BubR1 auto-phosphorylation in response to spindle microtubule capture by CENP-E is important for kinetochore functions in achieving accurate chromosome segregation. In the second part (Chapter 3), my colleague and I demonstrate a novel mechanism of mitotic spindle assembly in Xenopus egg …
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Genetic and biochemical analysis of the mammalian spindle assembly checkpoint
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 2001.
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Examining the Regulation of Cell Division by the Spindle Assembly Checkpoint
… The molecular surveillance mechanism called the spindle assembly checkpoint (SAC) inhibits chromosome segregation before all chromosomes are correctly attached (bi-oriented) on the microtubule spindle to prevent segregation errors that may lead to chromosomal instability and cell death. In this …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… with its essential co-activator Cdc20. The spindle assembly checkpoint (SAC) is a surveillance mechanism that monitors the chromosomes' interactions with the microtubules of the mitotic spindle apparatus. In response to even one erroneous attachment the affected kinetochore emits a "wait …
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Genetic and Cytological Analysis of the Spindle Assembly Checkpoint Component BUB-3 in DNA Damage Response
The spindle assembly checkpoint and the DNA damage response have been studied for decades as independent pathways exerting separate functions. However, crosstalk between these cellular processes has emerged. The extent of overlap between these pathways is still unknown, especially in multicellular …
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Histone H3 K4 Methylation Regulates The Spindle Assembly Checkpoint Through Direct Binding of Multiple Checkpoint Components and Cdc20
… unknown role in regulating mitosis through the Spindle Assembly Checkpoint. The Spindle Assembly Checkpoint ensures duplicated chromosomes are segregated correctly and each daughter cell receives one full copy of the genome. Our data show <em>SET1 </em>mutants and histone H3K4 mutants display a …
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B-cyclin/CDK Regulation of Mitotic Spindle Assembly through Phosphorylation of Kinesin-5 Motors in the Budding Yeast, <italic>Saccharomyces cerevisiae</italic>
… years that B-cyclin/CDK complexes regulate the assembly of the mitotic spindle and entry into mitosis, the full complement of relevant CDK targets has not been identified. It has previously been shown in a variety of model systems that B-type cyclin/CDK complexes, kinesin-5 motors, and the …
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Characterisation of ALADIN’s function during cell division
… It is now known that nucleoporins participate in spindle assembly, kinetochore organisation, spindle assembly checkpoint, and all processes important for genome integrity maintenance. This work demonstrates that the nucleoporin ALADIN participates in mitosis, meiosis and in cilia. In both mitosis …
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Spindle organization in three dimensions
… takes place on bipolar, microtubulebased spindles. Here, C. elegans is used to analyze spindle organization under both mitotic and meiotic conditions. First, the role of SAS-4 in organizing centrosome structure was analyzed. Partial depletion of SAS-4 in early embryos results in …
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Ncd Motor Tail Domain Interactions With Microtubules
… C-terminal motor protein that is involved in spindle assembly in oocytes during meiosis and in spindle maintenance in early embryos during mitosis. Ncd interacts with both "highway" and "cargo" microtubules (MTs) in meiotic and mitotic spindles through the action of ATP-dependent and …
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Regulation of mitotic spindle biogenesis in budding yeast
Assembly of the mitotic spindle is critically dependent on centrosome duplication (spindle pole body or SPB in yeast), separation of sister centrosomes and microtubule dynamics. Activation of Cdc28 (Cdk1) by tyrosine-19 dephosphorylation is essential for centrosome separation, the first step in …
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Control of Chromosome Segregation by the Aurora B Complex
… segregation during cell division requires spindle assembly around M-phase chromatin. In cells lacking centrosomes, such as those found in female meiosis, chromosomes themselves nucleate and stabilize microtubules in order to promote accurate spindle formation. Here we present a description …
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Investigation of the role of chromosome missegregation in embryo development and in tumorigenesis
… receive the correct number of chromosomes. The spindle assembly checkpoint prevents chromosome missegregation by arresting the cell before sister chromatid separation if any one chromatid is not properly attached to the spindle microtubules. Defects in chromosome segregation lead to the creation …
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Regulation of Chromosome Segregation by the Chromosomal Passenger Complex
… In eukaryotes, chromosome segregation depends on spindle formation. Chromosome-induced spindle assembly requires the chromosomal recruitment and activation of Aurora B. How this chromosome-activated kinase spatially disseminates signals that lead to spindle formation remains unclear. In the second …
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SMARCA4/BRG1-Inactivating Mutations as Potential Predictive Markers for Aurora Kinase A-Targeted Therapy in NSCLCs
… AURKA-centered, centrosome-independent, mitotic spindle assembly machinery becomes solely responsible for mitotic spindle formation and proper chromosome segregation during mitosis. DLG7, the only known protein specific to this centrosome-independent mitotic spindle assembly, is required for the …
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