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Showing 1 to 13 of 13 for “"spheroplasts"”.
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Optimizing a transformation system using spheroplasts of Escherichia coli 15T
Digitized by Kansas Correctional Industries
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Optimizing a transformation system using spheroplasts of Escherichia coli 15T
Digitized by Kansas Correctional Industries
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The Effect of Membrane Phase Transitions on The Osmotic Sensitivity of E. Coli Spheroplasts
Made available in DSpace on 2014-12-13T19:47:46Z (GMT). No. of bitstreams: 1 8009176.pdf: 3448486 bytes, checksum: a2f2b92589a58d1e5704aed35080250f (MD5) Previous issue date: 1979
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Development of a Transformation System for Caldariomyces Fumago and Expression of Heterologous Proteins
… Conditions were optimized for the generation of spheroplasts from C. fumago mycelium using the enzyme mixture Novozym 234. An osmotic medium was developed for maximum regeneration of the spheroplasts (40 to 50%) and sensitivity to antibiotics. Deoxycholic acid was shown to increase sensitivity …
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Spatial Control of CDC42 Activation Regulates Cell Width and Growth Zone Formation
… cell width. Cell wall removal generates round spheroplasts that can recover into normal rod-shaped cells, allowing the study of how a growth zone of a particular shape and dimensions is formed. Spheroplasts initially have a disorganized cytoskeleton and depolarized growth zone proteins. During …
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Chromatin Structure of Schizosaccharomyces Pombe: From the Lowest to the Highest Orders of Folding
… method for measuring the repeat length in viable spheroplasts. That result, together with other control experiments, rules out many possible artifacts. The measured value of 156 $\pm$ 2 bp is smaller than the length of DNA found in the chromatosome. Thus, the chromatosome cannot be the fundamental …
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Characterization of alternative enzymes in bacterial respiration
… gradient across the membranes were used with spheroplasts and membrane vesicles of F. johnsoniae. Our preliminary experiments with both the membrane vesicles and spheroplasts show no evidence of contribution to the proton motive force by the ACIII. An attempt was made to isolate the ACIII from …
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Homothallism in the Sordariaceae : Mating-type loci in selected species of Neurospora, Anixiella, and Gelasinospora
… when transformed into N.crassa sterile mutant spheroplasts.
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A Major Cell Envelope Protein Of Escherichia Coli K12: Purification And Properties
… shock treatments, or during the formation of spheroplasts. The protein was purified on columns of aspartate-Sepharose (A-S) an affinity matrix previously used for the isolation of dicarboxylate transport components. SEP was the major protein eluted when the A-S columns were equilibrated in low …
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Barriers to tuberculosis drug discovery: the mycobacterial cell wall
… of mycobacteria as the resulting organisms, spheroplasts, were capable of surviving in the absence of this seemingly essential protective layer. This presents a potentially novel form of intrinsic resistance whereby stripping of the cell wall could allow for tolerance to cell wall active …
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Exploring the Dynamics of Mechanosensitive Channels Utilizing a Post Translational Modification Approach
… In addition, patch clamp of giant bacterial spheroplasts has allowed for the study of channel activities in native membranes, and both channels have been functionally reconstituted into membranes and studied by electrophysiological single-channel analyses. However, even though well studied, …
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Characterization of swimming motility in a marine unicellular cyanobacterium
… TEM and gel electrophoretic analysis of spheroplasts, cell wall-enriched fractions from motile and nonmotile strains, and cell material collected following the application of a flagellar hook-basal body complex isolation technique to a motile Synechococcus strain provided no further …