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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"spectrin"”.
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The role of spectrin in Drosophila photoreceptor development.
Spectrin is a cytoskeletal protein that interacts with the plasma membrane, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In Drosophila, spectrin is composed of 3 subunits: α-spectrin, β-spectrin, and βH-spectrin. The …
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Function of Spectrin cytoskeleton in regulating epithelial architecture.
… the major component of both apical and lateral Spectrin cytoskeletons, the study was mainly focused on α-Spec. The study showed that α-Spec and β-Spec are essential in maintaining a mono-layered FE, but, contrary to previous reports, Spectrins are not required to control proliferation. …
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Calcium, actin and the spectrin skeleton: Responses to swelling and shrinking perturbations.
It was postulated that cells regulate their surface membrane in response to membrane tension gradients. Increases in membrane tension, brought about by swelling, should therefore elicit addition of membrane to the cell surface, whereas decreases in membrane tension, brought about by shrinking, …
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Evolutionary Dynamics of the Alpha-actinin Gene Family
The alpha-actinin gene family is a member of the spectrin superfamily of proteins. Other members of this superfamily include the a- and ~-spectrins, ~heavy spectrin and dystrophin. Each member of this superfamily has a unique actin cross-linking distance consequent to the specific number of …
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Molecular and genetic characterization of spinocerebellar ataxia type 5 (SCA5)
… conservation and sequencing, we discovered β-III spectrin (SPTBN2) mutations cause SCA5 in the American family and two additional ataxia families from France and Germany. In-frame microdeletions were identified in the American (39 bp) and French (15 bp) families within the third of 17 homologous, …
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Constitutive modeling of the finite deformation behavior of membranes possessing a triangulated networked microstructure
… features: the lipid bilayer and the underlying spectrin network. The lipid bilayer is analogous to a 2D fluid in that it resists changes to its planar area, yet poses little resistance to planar shear. A skeletal network of spectrin molecules is crosslinked to the lipid bilayer and provides the …
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Elucidating the reversibility of ataxia
… mutations in the SPTBN2 gene, encoding b-III spectrin, are implicated in spinocerebellar ataxia type 5 (SCA5) and spectrin-associated autosomal recessive cerebellar ataxia type 1 (SPARCA1), respectively. Our mouse model, lacking b-III spectrin (KO), mimics the progressive human phenotype …
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Cellular and Developmental Functions of The Xenopus Arvcf-Catenin:Kazrin Complex
… Instead, screening revealed that xKazrinA binds spectrin. This suggested a potential means by which xKazrinA localizes to cell-cell junctions, and indeed, biochemical assays confirmed a ternary xARVCF:xKazrinA:xβ2-spectrin complex. Functionally, I demonstrated that xKazrin stabilizes cadherins by …
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Characterization of erythrocyte membrane domains using fluorescence digital imaging microscopy
… ghosts immunofluorescently stained for spectrin revealed that the areas of Band 3 enrichment coincide with areas of spectrin enrichment. Also, spectrin aggregation by antibody crosslinking increased the Band 3 enrichment in the domains. However, trypsin or chymotrypsin treatment of …
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THE NON-ERYTHROID MEMBRANE SKELETON AND ITS ROLE IN MECHANOTRANSDUCTION
… A particular focus is thereby on the role of the spectrin membrane skeleton in transducing forces to and from non-erythroid mammalian cells. This spectrin based membrane skeleton has been the focus of much study in the context of red blood cells, as it determines their mechanical properties due to …
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Ca²⁺ and phosphoinositides regulations in α-actinin -4 F-actin binding.
… isoform of α-actinin that belongs to the spectrin superfamily. It comprises three functional regions: an N-terminal actin-binding region that consists of two calponin homology (CH) domains, a central region that consists of four copies of the spectrin-like repeat domain and a C-terminal …
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Tumor Antigens Revealed by Exome Seqeuncing Drive Editing of Tumor Immunogenicity
… sarcoma, d42m1, and identified mutant spectrin-β2 as the major rejection antigen. Moreover, we demonstrate that editing of d42m1 tumor cells occurs via a T cell-dependent immunoselection process that promotes outgrowth of variants lacking mutant spectrin-β2. Thus, the strongly …
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Proteins on the edge : transitions of structure ensembles in protein unfolding and protein-protein binding
… can, it seems, turn into major forces. Chains of spectrin repeats apparently rely on structure flexibility to achieve a smooth response to external force. Single molecule atomic force microscopy experiments on this domain, in accord with simulations, showed clear traces of structure fluctuation. …
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Regulation of Excitatory Neurotransmission, Synaptic Plasticity, and Learning by Cyclin-Dependent Kinase 5
… facilitated the calpain-mediated degradation of spectrin in vitro. Spectrin degradation and depolymerized actin levels were decreased in conditional Cyclin-dependent kinase 5 knockout hippocampus. These results implicate Cyclin-dependent kinase 5 dendritic in spine dynamics which is critical for …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
Nuclear envelope spectrin repeat proteins, or Nesprins, are a novel family of nuclear and cytoskeletal proteins with rapidly expanding roles as intracellular scaffolds and linkers. Nesprins are characterized by a central spectrin repeat (SR) rod domain and a C-terminal KASH domain, which acts as a …
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Applying a microfluidic 'deformability cytometry' to measure stiffness of malaria-infected red blood cells at body and febrile temperatures
… malaria, parasite proteins interact with the spectrin network of host RBCs, moderately stiffening the ring stage infected cells (rings). The subtle modification in the deformability of rings is however believed to be significant enough to trigger their retention by human spleen. In addition, …
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ADDUCINS AFFECT CORTICAL NEUROGENESIS BY REGULATING PROGENITORS¿ MORPHOLOGY AND FATE
… interest. ADD1, ADD2 and ADD3 anchor the spectrin-actin cytoskeleton to the cell membrane through their interaction with other transmembrane proteins. They have recently been associated with various neurodevelopmental disorders, such as ventriculomegaly and corpus callosum dysgenesis, …
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Obscurin Mediates Ankyrin Complex Formation In The Heart
… sites in its C-terminus. We also show that β1-spectrin, a protein that does not directly interact with obscurin or sAnk1.5, can be recruited to a macromolecular complex via an interaction with AnkG107/130. Lastly, both ankyrin isoforms localize to the M-line of cardiomyocytes suggesting that …
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