Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"spastin"”.
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The interconnected roles of spastin and protrudin in endosomal sorting
… spastic paraplegia (HSP)- associated proteins, spastin (SPG4) and atlastin-1 (SPG3A), as essential for endosomal tubule fission. I investigated the functional interaction of spastin and protrudin and found that protrudin, an ER-associated adaptor molecule involved in anterograde membrane …
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Investigating the function of the hereditary spastic paraplegia protein spastin in the endomembrane system
… HSP, mutations in SPAST, encoding the protein spastin, are by far the most common cause of pathology. Spastin functions as a microtubule remodelling enzyme by using energy derived from ATP hydrolysis by its ATPase domain. The location of this activity is governed by spastin’s localisation …
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Using Chemical Probes to Examine Cellular Activities
… thesis in which I (i) explore the role of spastin during late anaphase and nuclear envelope reformation using our recently developed spastin inhibitor and (ii) use inhibitor resistance to identify morphological markers of drug resistance via high-content microscopy. In the first part of my …
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Molekulare Charakterisierung des COPS5-Gens und seines Genproduktes als Kandidat für die Spastische Spinalparalyse
… kommt dem SPG4-Gen mit seinem Genprodukt Spastin zu welches für ca. 40 % der autosomal-dominanten HSPs verantwortlich ist. In einem Yeast Two Hybrid (Y2H) konnte die Interaktion seines Genprodukts Spastin mit COPS5 gezeigt werden. Ziel dieser Arbeit war deshalb eine Charakterisierung des …
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Analyzing Resistance to Design Chemical Inhibitors of AAA Proteins
… first potent and selective chemical probe for spastin, a microtubule-severing AAA protein needed for cell division and intracellular vesicle transport. I confirmed the predicted binding mode of spastazoline analogs by X-ray crystallography and used these high-resolution structural models, along …
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Molekulare Charakterisierung von ZFYVE27 und Identifizierung von Deletionen in SPG4
… und an Endosomen gebunden vor und ist mit Spastin co-lokalisiert.Die für das Deletionsscreening verwendete Real-Time-PCR erwies sich unter den angewandte Bedingungen als nicht verlässlich genug, und obwohl Ergebnisse anderer Arbeiten eben diese in AD-HSP-Patienten nachweisen konnten, gelang …
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An analysis of the role of microtubules during tubulogenesis in Drosophila
… gland placode using the MT-severing protein Spastin results in defects in apical constriction, and at later stages tube lumen defects. Wild type cells generate an apical medial actomyosin meshwork that likely drives apical constriction. MT ends present at the apical surface colocalise with …
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Testing roles of Hereditary Spastic Paraplegia genes in axonal endoplasmic reticulum modelling in Drosophila
… common mutations in HSP affect proteins of the spastin, atlastin, REEP and reticulon (RTN) families, which possess hydrophobic hairpin domains in the ER membrane to help confer curvature on tubular ER. Drosophila mutants lacking members of RTN and REEP families exhibit partial ER fragmentation …
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Testing roles of Hereditary Spastic Paraplegia (HSP) proteins in organization of axonal endoplasmic reticulum (ER) and ER-mitochondria contacts
… in HSPs. These genes encode ER-shaping proteins: spastin (SPG4), atlastin (SPG3A), Receptor Expression Enhancing Protein 1 (REEP1/SPG31) and reticulon (SPG12). These proteins share a common feature of one or two intramembrane hairpin domains that can recognise or drive curvature of the ER …