Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"spacer acquisition"”.
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A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
… at the level of local clusters. I then performed spacer mapping using spacers derived from the type VI systems CRISPR arrays. This revealed that local clusters of Type VI systems target a mixture of plasmids and phages, even in cases where the type VI CRISPR-array was found to be prophage encoded. …
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A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
… at the level of local clusters. I then performed spacer mapping using spacers derived from the type VI systems CRISPR arrays. This revealed that local clusters of Type VI systems target a mixture of plasmids and phages, even in cases where the type VI CRISPR-array was found to be prophage encoded. …
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Generation of Memory of Infection During the CRISPR-Cas9 Immune Response
… Upon infection, short phage sequences known as spacers integrate between CRISPR repeats and are transcribed into small RNA molecules that guide the Cas9 nuclease to the viral targets (protospacers). Streptococcus pyogenes Cas9 cleavage of the viral genome requires the presence of a 5′-NGG-3′ …
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Cas9-Primed Adaptive Immunity During the CRISPR-Cas Response
… intercalated with variable sequences known as spacers. Following viral infection, these sequences are acquired from the viral genome and integrated in the CRISPR array as new spacers. Spacers are then transcribed into CRISPR RNAs (crRNAs) that direct the Cas nucleases to destroy the invader …
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Restriction-Modification and CRISPR-Cas Systems: Cooperation Between Innate and Adaptive Immunity in Prokaryotes
… separated by unique DNA sequences known as spacers. During phage infection, short DNA fragments are taken from the viral DNA and integrated into the CRISPR locus to form new spacers. These sequences are then transcribed into CRISPR RNAs (crRNAs). In type II-A CRISPRCas systems, the crRNAs …
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Functional Organization of Molecular Memories in the CRISPR-Cas Immune System
… of direct repeats intercalated by variable spacer sequences (~30bp) of foreign origin. Upon infection, spacers are excised from the foreign genome and integrated into the array. The array is then transcribed and parsed into individual CRISPR RNAs, each containing a single spacer sequence, …
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An expanded search for RNA-programmable genomic engineering effectors
… relied on protein proximity to CRISPR-associated spacer acquisition proteins Cas1 and Cas2, this approach was seeded on CRISPR arrays alone. What resulted was the discovery of a potential new Class 2 CRISPR system, with two subtypes as characterized by distinct putative accessory proteins. …
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Rarely Acquired Type II-A CRISPR-Cas Spacers Mediate Anti-Viral Immunity Through the Targeting of a Non-Canonical PAM Sequence
… interspersed with short fragments of phage DNA (spacers). The cas genes code for proteins that regulate 1) the acquisition of new spacers during phage infection ("immunization") and 2) the use of spacer transcripts to identify and degrade complementary foreign DNA sequences (protospacers) to …
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Modulation of the CRISPR-Cas9 Immune Response
… systems provide adaptive immunity through the acquisition of short DNA sequences from invading phages, known as spacers. Spacers are inserted into the CRISPR locus and serve as templates for the transcription of guides used by RNA-guided nucleases to recognize complementary sequences of the …
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Recovering from Dormancy: Fixation of CSM6-Inducing Spacers in the Bacterial Population
… repeat sequences separated by short "spacer" sequences which are derived from prior invading genetic material and form the basis of these acquired memories. During an immune response, spacer sequences are transcribed into guide RNAs and will recognize complementary sequences on …