Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"southern-hybridisation"”.
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Phosphoenolpyruvat-Carboxylasen in Solanum tuberosum : Charakterisierung und Expression
… a S.tuberosum genomic phage bank by plaque and Southern hybridisation. The inserts of the phage clones were subcloned and the complete genomic sequence of the stppc2 gene (8438 bp; EMBL Accession Number AJ011844) and the promoter sequence of the stppc1 gene (873 bp; EMBL Accession Number …
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Elucidation of the osmoregulatory locus, ompRZ, in Erwinia chrysanthemi
… primers to generate an E. coli envZ clone. Southern hybridisation analyses, using an ompR probe, confirmed the presence of an ompR homologue in E. chrysanthemi. An E. chrysanthemi genomic library was thus constructed and screened and a clone homologous to the ompR probe was isolated. The …
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Retroviruses in the common brush-tailed possum (Trichosurus vulpecula)
… element in the possum genome, as determined by Southern hybridisation. However, integration sites appeared to be variable between possums, suggesting recent (or ongoing) retrotranspositional activity. Both PCR and Southern hybridisation analyses suggest that TvERV-K elements shorter than those …
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Characterisation of badnavirus sequences in West African yams (Dioscorea spp.)
… K09 and U12) that contain integrated sequences. Southern hybridisation results using individual DGGE band partial RT-RNaseH sequences (NGb4_Dr, NGb5_Dr and NGb6_Dr), supported integration of badnavirus sequences in genomes of D. rotundata breeding lines. Fluorescent in situ hybridisation (FISH) …
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STUDIES ON THE EFFECTS OF SOME FLAVONOIDS ON DNA TRANSCRIPTION AND CLONING OF LIPOAMIDE DEHYDROGENASE GENE
… the human cDNA library were obtained following Southern hybridisation of the library with a 32-P labelled 27 mer synthetic probe. Enzymological studies indicated the presence of a correctly inserted gene which is expressed following IPTG induction. Double digestion with restriction enzymes KpnI …
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Nachweis und genetische Analyse der cag Pathogenitätsinsel von Helicobacter pylori mit neuen, molekularbiologischen Methoden
… PCR-Analyse und der DNA-Makroarray auf Basis der Southern-Hybridisierung geeignete diagnostische Werkzeuge zur Analyse der cag-PAI von H. pylori darstellen. Mit Hilfe dieser Methoden konnte die DNA von insgesamt 23 natürlichen H. pylori-Isolaten nicht nur auf die An- oder Abwesenheit der gesamten …
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Copper tolerance of Listeria monocytogenes strain DRDC8.
… ion transport in L. monocytogenes (Bell, 2002). Southern hybridisation analysis was used to confirm that the ctpA gene is carried on plasmid pCT100 in strain DRDC8. In addition, evidence to suggest that ctpA was encoded by bacteriophage DNA was not obtained. Furthermore, sequence analysis of DNA …
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Studies on the regulation of nitrogen metabolism in clostridium acetobutylicum NCP 262 and clostridium beijerinckii NCIMB 8052
… of high concentration of cas amino acids. Southern hybridisation experiments identified homologues of ginA, glnR and gltA in C. beijerinckii NCIMB 8052, and a clone was isolated from a partial gene library of this organism which complemented glutamine auxotrophy in E. coli YMCll. The clone …