Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"somatic hypermutation"”.
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Mechanism of Mismatch Repair Induced Mutagenesis in Somatic Hypermutation
… phase of a humoral immune response. However, somatic hypermutation of the rearranged V region in the immunoglobulin locus generates new antibody affinities, accompanied by the selection of B cells that produce superior antibody affinities. Somatic hypermutation is initiated by the conversion …
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A novel genomics and bioinformatics approach to assess immunoglobulin and T cell receptor rearrangements and somatic hypermutation in lymphoproliferative disorders
… and affinity maturation for diversification. Somatic hypermutation (SHM) is a B cell specific affinity maturation process whereby point mutations are intentionally inserted into the genomic sequence to marginally alter the confirmation of immunoglobulin (Ig) receptors. SHM is part of normal B …
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The evolution of the autoantibody response in Graves’ disease
… precursor B cell clone which diversified through somatic hypermutation. The shared germline genes were expressed as recombinant (r) Fab germline and did not display measurable binding to TSHR in three assay systems: radioreceptor assay, flow cytometry and cAMP stimulation bioassay. Hence the …
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Isolation and engineering of a high affinity antibody against P-selectin glycoprotein ligand-1 (PSGL-1)
… strategies exploited by B cells such as somatic hypermutation, receptor revision (... CDR replacement), receptor editing (chain shuffling), and amino acid insertions and deletions. Together with yeast surface display and flow cytometric screening (FACS), this approach has been used to …
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STEPWISE DIFFERENTIATION OF IL21-PRODUCING FOLLICULAR HELPER T CELLS REVEALS DISTINCT DEVELOPMENTAL AND FUNCTIONAL STATES
… germinal center formation, maintenance and somatic hypermutation at distinct stages of the germinal center response to a SARS-CoV-2 protein vaccine. Together, these studies demonstrate the transitionary stages of Tfh development and how control of progression through these stages regulates …
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Regulation of Immunoglobulin Gene Diversification By Noncoding RNA's
… of gene expression that requires significant somatic alteration of the genetic code. B lymphocytes perform somatic hypermutation (SHM) and class switch recombination (CSR) of the immunoglobulin locus to generate an antibody repertoire diverse in both affinity and function. These somatic …
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Role of AID and microRNA-155 in c-myc-IgH Translocations
… in mature B cells undergoing AID dependent somatic hypermutation (SHM), duplications or deletions that would necessitate a double strand break make up 6% of all the Ig V-JH region associated somatic mutations. Furthermore, DNA breaks can be detected at this locus in B cells undergoing SHM. …
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Immune Transcriptome and B cell receptor repertoire in COVID-19
… BCRs bearing IgG1/3 and IgA1 isotypes increased, somatic hypermutation (SHM) was markedly decreased and, in patients with severe disease, expansion of IgM and IgA clones were observed. In contrast, after vaccination the proportion of BCRs bearing IgD/M isotypes increased, SHM was unchanged and …
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In Vitro Evolution of Antibody Affinity using Libraries with Insertions and Deletions
… diversification through the process called somatic hypermutation (SHM) leads to antibodies with improved affinity and specificity. Sequence diversification by SHM is mainly achieved by introduction of point substitutions and a small percentage of insertions/deletions (indels). Although the …
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The Role of Activation-Induced Cytidine Deaminase in Antibody Diversification and B Cell Malignancies
… cytidine deaminase (AID) is indispensable for somatic hypermutation (SHM) and class switch recombination (CSR) in B cells. SHM alters the affinity of an antibody for its corresponding antigen by introducing point mutations within the variable region of the immunoglobulin (Ig) gene heavy chain …
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Translational regulation in aggressive B-cell lymphomas
… process where B-cells undergo recombination and somatic hypermutation of immunoglobulin genes in response to antigen stimulation. This essential component of the adaptive immune system is associated with cycles of intensive proliferation and selection, which carries a risk of malignant …
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Mechanisms Underpinning Functional Resident Humoral Lung Immunity
… lymphoid organs fail to support efficient somatic hypermutation, thereby producing more broadly reactive antibodies. CD69 is an early-activation marker which is understood to be upregulated rapidly upon lymphocyte activation and can promote tissue-retention of T cells. I hypothesise that …
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Characterization of Variable Region Structures and Antigen-Binding Properties of OxLDL-Specific Human Monoclonal Antibody Isolated from PBMC and Atherotic Plaques of Atherosclerosis Patients
… was 84~98%, those of VL was 86~98%. Evidence of somatic hypermutation and class switch recombination was also obtained by analyzing the local expression of mRNA transcripts encoding AID in the atherosclerotic plaque. CONCLUSION: The oxLDL-specific human monoclonal antibody Fabs which have 10~1000 …
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Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution
… of antibodies through initiation of somatic hypermutation (SHM), and class switch recombination (CSR), by the C deaminations in immunoglobulin genes. Activity of these enzymes requires scanning of single-stranded (ss) DNA substrates to locate deamination motifs and stochastically …
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B cell memory in STAT3 Hyper-IgE Syndrome: Cytokine integration, IgE regulation, and the architecture of human memory B cell subsets
… that the transcriptional signature and somatic hypermutation profile of IgE+ B cells resembled that of a recently described allergy-associated subset of human IgG1+ B cells (termed type 2 memory B cells; MBC2s). Furthermore, our analyses revealed that BCR signalling is differentially …
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Targeting the CD4 Binding Site of HIV
… group of light chains bearing specific somatic mutations that enhance neutralizing activity. The data suggest that vaccination to elicit broad anti-HIV-1 antibodies will require immunization with a succession of related immunogens. Although CD4bs bNAbs are attractive candidates for …
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Genome-Wide Characterization of the Effects of Nucleic Acid Modifying Enzymes: Cytidine Deaminases and DNA Methylation
… diversification in germinal center B cells: somatic hypermutation (SHM), in which mutations are introduced into immunoglobulin (Ig) genes, and class-switch recombination (CSR), in which genomic constant regions are recombined to encode antibodies of different isotypes. Both of these processes …
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Épissage alternatif et mutations ponctuelles de Pax5 chez la leucémie lymphoîde chronique et les lymphomes de cellules B
… resulting in the over-expression or aberrant somatic hypermutation of the Pax5 gene, but recent studies have suggested the possibility that there is an altered modulation of human BSAP isoforms in certain B-cell cancers. This hypothesis is still very new, however, and has not been thoroughly …
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Mapping the Genomic Context of Mutagenesis
… TensorSignatures reproduces a signature of somatic hypermutation generating highly clustered variants around the transcription start sites of active genes in lymphoid leukaemia, distinct from a more general and less clustered signature of Polη-driven translesion synthesis found in a broad …
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