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Showing 1 to 20 of 20 for “"somatic embryos"”.
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Transformation of Somatic Embryos of Soybean With Chitinase and Beta-1,3-Glucanase Genes via Particle Bombardment
… $\beta$-1,3-glucanase gene were transformed into somatic embryos of soybean using the conditions described above. Enzyme activity assays indicated that the transformed progeny had higher activity than that of the wild type and homozygous progeny had higher activity than heterozygous progeny. …
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The identification and characterisation of key genes involved in somatic embryogenesis in wheat (Triticum, aestivum L.)
… embryonic development, via a process known as `somatic embryogenesis' (SE). On removal of auxin these totipotent cells are then capable of re-differentiating and regenerating into full adult plants. Although well characterised physiologically, the molecular mechanisms that regulate this …
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Somatic embryogenesis in Pinus radiata
Somatic embryogenesis of Pinus radiata has been seen as an attractive alternative to traditional propagation methods. This is due to its potential for high multiplication rates and rejuvenation, as well as its compatibitity with molecular techniques. To date no commercial production of somatic …
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Callus Development and Organogenesis in Cultured Explants of Cowpea (Vigna unguiculata (L.) Walp
… embryogenic suspension cultures were evaluated. Somatic embryos were formed in suspension cultures. Attempts to convert and germinate the somatic embryos resulted in the formation of callus or formation of appendages on the somatic embryos or in the death of the embryos. The appendages formed …
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PROPAGATION OF PLANTS BY SOMATIC EMBRYOGENESIS
… would be reduced by utilising the phenomenon of somatic embryogenesis for plant regeneration (Jones, 1984, Biochem. Soc. Symp. 98: 222-232). Although many publications describe how mbryogenesis may be induced in particular cases (reviewed by Williams and Maheswaran, 1986, Ann. Bot. 57: 449-462), …
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The cryopreservation of <i>Picea sitchensis </i>germplasm
… embryogenic suspensor masses, to matured somatic embryos and whole tissue shoot-tip apices. Before cryopreservation was initiated in vitro cultures were stabilised and characterised at UAD. Shoot cultures showed different growth responses between genotypes and between culture locations, …
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Development of in vitro regeneration and gene transfer systems for conifer species
… of S. sempervirens, shoot organogenesis and somatic embryogenesis were induced. The influence of genotype and various cytokinins (BA, BPA, and TDZ) in combination with 2,4-D were investigated. Among the cytokinins tested, 20 $\mu$M BPA with 5 $\mu$M 2,4-D produced the highest frequency of …
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Propagation of asparagus (Asparagus officinalis L.) via somatic embryogenesis
This thesis reports the development of somatic embryogenesis system for asparagus (Asparagus officinalis L.). Somatic embryogenesis is important as an efficient system for clonal propagation. To achieve this technique, the first step is the initiation of callus followed by the induction of the …
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Somatic embryogenesis in southern and tropical pine species: Loblolly pine (Pinus taeda), Longleaf pine (P. palustris) and Oocarpa pine (P. oocarpa)
… establish an improved and reliable protocol for somatic embryogenesis in 1) Pinus taeda and Pinus palustris; pine species of high value for commercial applications and germplasm conservation supported through breeding programs at The Virginia Department of Forestry (Chapter III); and 2) Pinus …
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Modification of Vegetable Soybean Sugar Content via Gene Transformation
To modify soybean sugar content, somatic embryos of soybean cvs. Peterson Jade, Daelip Hakja (PI 157.419), Yakumo Meaka (PI 417.468), Kinshu (PI 549.065) Kaohsiung No.1 (PI 536.546) and Jack were transformed with yeast invertase (SUC 2) and $\alpha$-galactosidase (agl A) from Aspergillus niger …
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Identification, isolation, expression analysis and molecular characterization of nine genes key to late embryogenesis in Loblolly pine
… how and why only a very small percentage of somatic embryos develop past the late embryogeny phase of embryogenesis. In this work, we have identified genes that have been demonstrated to be required for late embryonic development in the model plant system Arabidopsis thaliana. These genes …
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Applied Biotechnology for in Vitro Production of Carica Papaya L
… in the medium. A higher frequency of somatic embryogenesis was achieved in a shorter time in a liquid system than reported previously for other systems. The presence of abscisic acid (ABA) in the maturation medium enhanced embryo size and increased the percentage developing into …
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CELL DIVISION AND EMBRYOGENIC COMPETENCE IN ALFALFA {Medicago falcata L.)
… exploited a procedure for the production of somatic embryos directly from alfalfa leaf explants. Chopped leaf fragments were incubated in a liquid medium containing 2,4-D. The organisation of the swollen leaf tissue was disrupted and after 15 days the globular clusters of small dividing cells …
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Somatic Embryogenesis of Pathogen-Free Horseradish (Armoracia Rusticana) Plants in Vitro
… to produce whole plants from single cells via somatic embryogenesis. To initiate somatic embryogenesis, the Illinois Horseradish 'Doll' cultivar ('Doll') was tested with 2,4-D (0.25 to 1.0 mg/l) and BA in vitro. Somatic embryogenic callus was successfully induced from leaf explants on MS medium …
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A microculture approach towards enhanced productivity and genetic improvement of pecan (Carya illinoensis)
… basis. Axenic cultures from mature pecan embryos were established in vitro by reducing the water availability in the initial explanting media. This enabled study of the regenerative capacity of the mature pecan embryo explants, and the factors affecting shoot multiplication and rooting. A …
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Somatic embryogenesis of pathogen- free horseradish (Armoracia Rusticana) plants in vitro
… to produce whole plants from single cells via somatic embryogenesis. To initiate somatic embryogenesis, the Illinois Horseradish ‘Doll’ cultivar (‘Doll’) was tested with 2,4 -D (0.25 to 1.0 mg/l) and BA in vitro. Somatic embryogenic callus was successfully induced from leaf explants on MS …
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WHO: A Novel Wuschel-Like Homeodomain Transcription Factor in Arabidopsis
… as aberrant floral organs. Moreover, callus and somatic embryos formed regularly from the mutant petioles and hypocotyls on normal MS medium without any external phytohormone treatment. Morphological studies showed that mutant roots had a radial organization defect, producing 7 cortical and 7 …
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MOLECULAR ASPECTS OF DIRECT SOMATIC EMBRYOGENESIS IN ALFALFA (MEDICAGO)
Somatic embryogenesis follows the developmental pattern of the zygote in vitro so it provides a model for early plant development which is more accessible for experimental manipulations. Most of the fundamental research in this area has exploited indirect embryogenic systems which require cells to …
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Palmitoyl-acyl Carrier Protein Thioesterase in Cotton (Gossypium hirsutum L.): Biochemical and Molecular Characterization of a Major Mechanism for the Regulation of Palmitic Acid Content
… elevated levels of palmitic acid in transgenic somatic embryos compared to control embryos. Expression of the anti-sense FatB cDNA in transgenic cotton plants produced some plants with a dwarf phenotype. These plants had significantly smaller mature leaves, all with smaller cells, suggesting …
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Soybean Mosaic Virus Hc-Pro Enhances Viral Symptoms, Bean Pod Mottle Virus and Transgene RNA Stability, siRNA Accumulation, and Reduces Soybean Seed Production
… enhanced the recovery of hygromycin-resistant somatic embryos from immature soybean cotyledonary explants co-cultured with Agrobacterium . Expression of SMV HC-Pro in transgenic plants partially stabilized the expression of a beta-glucuronidase (GUS) transgene that was susceptible to PTGS. SMV …