Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"somatic cell nuclear transfer"”.
-
Somatic cell nuclear transfer in zebrafish
Thesis (Ph. D.)--Michigan State University. Comparative Medicine and Integrative Biology, 2010
-
Effects of somatic cell nuclear transfer on transcripts related to IGF signaling, EGF signaling and cell adhesion during the peri-implantation period in cattle
Up to 70% of somatic cell nuclear transfer (SCNT)–derived bovine pregnancies are lost during the peri-implantation period of gestation. Given the physiology of blastocyst implantation and clinico-pathological features that characterize SCNT pregnancies, we hypothesized that insulin-like growth …
-
DNA methylation in the early porcine embryo
… sperm injection, parthenogenetic activation, and somatic cell nuclear transfer are powerful procedures in the production of animals for agriculture, basic research, and biomedical research. Research using these techniques has produced important insights into the basic mechanisms of gametogenesis, …
-
Involvement of mitochondrial transcription factor A (TFAM) in porcine gametogenesis and preimplantation embryo development
… activated oocytes and zygotes reconstructed by somatic cell nuclear transfer (SCNT). Boar sperm TFAM was found to be posttranslationally modified by ubiquitination and relegated to sperm tail principal piece in fully differentiated spermatozoa. These data suggest that mitochondrial transcription …
-
DNA methylation in early mammalian development
All the cells in the body contain the same genome yet showcase drastically different phenotypes. This is the result of different transcriptional programs, which are partly controlled by epigenetic modifications, including DNA methylation. In this thesis, I analyze genome-scale DNA methylation …
-
Quiescence induces epigenetic changes in bovine fibroblasts and improves their reprogramming into cloned embryos
Cloning by somatic cell nuclear transfer (SCNT) forces cells to lose their lineage-specific epigenetic marks and become totipotent again. This reprogramming process often results in epigenetic and transcriptional aberrations that compromise development. Development rates after SCNT can thus serve …
-
Induced pluripotency and reprogramming by defined factors
… genome is the foundation for epigenetics and cellular differentiation. Somatic cell nuclear transfer experiments (SCNT) provided unequivocal proof of nuclear equivalency between adult somatic cells. However the molecular mechanisms of somatic cell reprogramming have remained largely a mystery. …
-
CRISPR/CAS9 mediated gene editing for the improvement of beef and dairy cattle
… genes to create a break in the DNA of fibroblast cells from Nelore and Angus cattle. Cells were also supplied with a 90 nucleotide single stranded-oligonucleotide repair template, which allows for homology directed repair to introduce the desired substitution at those loci. Cells from these cattle …
-
Effect of simple preservation treatments on porcine chondrocyte isolation
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-20 without embargo terms
-
The Placenta Methylome: Novel Insights into DNA Modifications in the Human Placenta
… of the placenta is unique as compared to other somatic tissues and is marked by global hypomethylation. The placental methylome has been shown to modulate developmental processes, intrauterine exposures, and placental function. However, the precise mechanisms underlying DNA methylation in …
-
Enhanced transcriptome profiling and biomarker discovery using meta-analytical techniques
… technologies, artificial insemination or somatic cell nuclear transfer. The availability of conditions not present in all studies allowed testing of the novel meta-analysis model. The availability of the two reproductive technologies across all studies allowed the comparison of standard …