Global ETD Search
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Showing 1 to 4 of 4 for “"solvent isotope effects"”.
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Mechanistic investigation of human cytochromes P450 involved in hormone biosynthesis
… of the two. To this end, I investigated kinetic solvent isotope effects on steady state turnover for lyase reaction on OH-PROG. Further, cryo-trapped peroxo-anion intermediate was spectroscopically followed as the samples were thermally annealed. Resonance Raman spectroscopy (rR) on the trapped …
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Flavin Amine Oxidases from the Monoamine Oxidase Structural Family Utilize a Hydride Transfer Mechanism
… results in a 30- fold decrease in kcat/KO2. Solvent isotope effects and proton inventories are consistent with Lys315 accepting a proton from a water molecule hydrogen-bonded to the flavin N5 during flavin oxidation. Steady-state and transient kinetic studies of para-substituted …
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The role of cysteine 230 and lysine 238 of biotin carboxylase in the deprotonation of biotin and synthesis of bisubstrate analogy inhibitor of carboxyltransferase
Acetyl-CoA carboxylase catalyzes the first step in the synthesis of fatty acids. The Escherichia coli form of the enzyme consists of a biotin carboxylase protein, a biotin carboxyl carrier protein, and a carboxyltransferase protein. This enzyme uses the cofactor biotin as a carboxyl carrier. In …
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Exploring the reactivity of bacterial multicomponent monooxygenases
… transfer. The processes exhibit normal kinetic solvent isotope effects (KSIEs) of 2.0 and 1.8, respectively, when the reactions are performed in D20. Mechanisms are proposed to account for the observations of these novel intermediates and the proton dependencies of P* to Hroxo and Hproxo to Q …