Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"solid-phase peptide synthesis (SPPS)"”.
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Design and synthesis of handles for solid-phase peptide synthesis and convergent peptide synthesis
The recent popularity of methods for solid-phase peptide synthesis that use the 9-fluorenylmethyloxycarbonyl (Fmoc) group for N<sup>α</sup>-amino protection has created a need for compatible anchoring linkages and handles. In an effort to develop mild new methods for use in solid-phase peptide …
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Discovery and functional study of a bioactive peptide QUB-1892 from the defensive skin secretion of The Edible Frog (Pelophylax eculentus)
… kl esculentus and was named QUB-1892. The mature peptide was synthesised by solid-phase peptide synthesis (SPPS). RP-HPLC and MALDI-TOF MS were used to purify and analyse the mature peptide. Its minimal inhibitory concentration (MIC) was obtained by using different micro-organisms and its …
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Using peptides to examine the interaction interface between Aspartate transcarbamoylase and Dihydroorotase in pyrimidine biosynthesis in Aquifex aeolicus
… respectively, in <em>de novo</em> pyrimidine biosynthesis. Both enzymes form an active complex (DAC) in <em>Aquifex aeolicus</em>, where loop A of DHOase interacts with a domain of ATCase. The main objective of this work is to determine the function of specific residues of loop A in DAC …
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Analysis of a novel antimicrobial peptide QUB-1746 from the skin secretion of the frog, Kassina senegalensis
… such as biogenic amines, steroids, alkaloids, peptides and proteins. In this thesis, the full-length cDNA sequence of a novel antimicrobial peptide, named QUB-1746, was cloned from a Kassina senegalensis skin secretion derived cDNA library through ‘shotgun’ cloning. The mature peptide was …
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The discovery and evaluation of a novel bioactive peptide, QUB-3112, from the defensive skin secretion of Hylarana guentheri
… compounds, including bufogenins, steroids, peptides, etc. To date, many peptides with various bioactivities have been found in frog skin secretions. Among these, the majority are antimicrobial peptides and a novel one of these was studied in this thesis in order to contribute to the study of …
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Identification and functional study of a novel bioactive peptide QUB-1761 from the venom of the Brazilian yellow scorpion, Tityus serrulatus
Scorpion venom contains a variety of peptides exerting broad bioactivities. In this thesis, QUB-1761, a non-disulphide bridged peptide (NDBP), was discovered in the venom of the Brazilian yellow scorpion, Tityus serrulatus (T. serrulatus). The peptide was identified through molecular cloning by …
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Discovery and bioactivity assessment of a peptide QUB-1331 from the skin secretion of the Senegal running frog (Kassina senegalensis)
Since more and more antimicrobial peptides exhibited enormous potency in inhibiting the growth of microorganisms, especially bacteria and fungi, they have been regarded as alternative therapeutic reagents for various treatments in clinic. <br/><br/>In this research, a novel peptide QUB-1331 …
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QUB-1951: a bioactive peptide from the skin secretion of the Chinese piebald Odorous frog, Odorrana schmackeri
Peptides derived from the secretion of various frogs and toads show a great diversity and potential for discovery and development of novel therapeutics. In recent years, peptide-based drugs have been in the spotlight due to their high therapeutic potential combining high efficacy with low toxicity. …
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The discovery of a novel peptide, QUB-2023, from Rana amurensis
The abundant antimicrobial peptides (AMPs) discovered in amphibian skin secretions have raised the interest of many researchers not only because of their broad spectrum of actions against many clinically-relevant microorganisms but also their superiority over conventional antibiotics.<br/><br/>This …
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Isolation, characterisation and molecular cloning of a bioactive peptide, QUB 1675, from the defensive skin secretion of Odorrana livida
… of the genus Odorrana harbour a large number of peptides in their skin secretions. In this thesis, a novel peptide containing 14 amino acids (TSRCYVYRLKVVCS) was discovered in the skin secretion of Odorrana livida. It was named systematically as QUB 1675. The sequence of the cDNA encoding the …
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The discovery and bioactivity evaluation of a novel peptide, QUB-2652, from the skin secretion of the South American frog, Phyllomedusa palliata
… development of new antibiotics. Antimicrobial peptides (AMPs) from amphibian skin secretions possess broad-spectrum antimicrobial activities through the mechanism that is unlikely to induce antibiotic resistance. Thus, AMPs are great candidates for new antibiotics. <br/>In this study, the …
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A bioactive peptide QUB2870 from the defensive skin secretion of the Heilongjiang brown frog (Rana amurensis)
… secretions of amphibians consist of a variety of peptides with different bioactivities which have important roles in supporting amphibians to survive in complex environments and against predators. Many of these peptides possess anticancer, antiviral, trypsin inhibitory and antimicrobial …
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QUB-2614: a bioactive peptide from the defensive skin secretion of Odorrana schmackeri
… compounds, such as anti-microbial peptides (AMPs) and anticancer peptides (ACPs), which are used by biomedical researchers as a source of potential novel drug leads or pharmacological tools.<br/><br/>In this thesis, an AMP was identified in the skin secretion of Odorrana schmackeri …
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QUB-1331: a bioactive peptide from the skin secretion of the Chinese piebald odorous frog (Odorrana schmackeri)
Peptides from amphibian skin secretions are widely studied because of their unique physiological and neurotransmitter roles, and are commonly acknowledged as one of the most indispensable elements during the survival and evolution of amphibians. Among these peptides, bradykinin-related peptides …
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The discovery of a bioactive peptide, QUB-1957, from the defensive skin secretion of the Chinese piebald odorous frog, Odorrana schmackeri
… source where increasing numbers of antimicrobial peptides (AMPs) have been discovered.<br/><br/>In this study, we discovered a novel peptide QUB-1957 from the skin secretion of the Chinese Piebald odorous frog, Odorrana schmackeri, by the method of “shotgun cloning”. After the Solid-Phase Peptide …
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The discovery of a novel peptide, QUB-2274, from the defensive skin secretion of Rana amurensis
… especially the broad-spectrum antimicrobial peptides, which have attracted increasing attention as potential therapeutic agents.<br/><br/>In this thesis, a novel bioactive peptide was discovered in the skin secretion of Rana amurensis. This skin secretion was collected by mild electrical …
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Identification and activity assessment of a bioactive peptide, QUB-1938, from the skin secretion of the Broad-folded frog (Hylarana latouchii)
… the main topic was to extract a novel bioactive peptide from the skin secretion of the frog, Hylarana latouchii. The detailed steps of which are as follows:1) Molecular cloning begins with the extraction of mRNA from skin secretions of target frog and the construction of its cDNA library. The …
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The discovery and bioactivity evaluation of a novel peptide, QUB-1925, and its modified analogue, QUB-2067, from the defensive skin secretion of the frog, Phyllomedusa coelestis
… a great number of bioactive frog skin peptides have been isolated and structurally characterised. These bioactive frog skin peptides, which play a crucial role in protecting frogs from pathogenic microbes’ invasion and natural predators’ attack, are thought to be highly potential of …
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Assessing bioactivity of a bioactive peptide from the defensive skin secretion of Odorrana schmackeri
… to the range of pharmacologically active peptides present, skin secretions contain a broad spectrum of antimicrobial peptides (AMPs). So far, these peptides have been studied in only a few species and study of additional species is expected to find further new AMPs. Natural AMPs isolated …
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Molecular cloning and bioactivity assessment of a bioactive peptide QUB-1509 from the skin secretion of the Asian frog Hylarana guentheri
… significant. Temporins are among the smallest peptides that have been discovered to disrupt the bacterial membrane directly which makes it difficult for bacteria to develop resistance.<br/><br/>In this study, the antimicrobial peptide QUB-1509, which was isolated from the skin secretion of the …
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