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Showing 1 to 20 of 137 for “"solid phase peptide synthesis"”.
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Advancements in polymer resins for solid-phase peptide synthesis
The use of polymeric supports in solid-phase peptide synthesis has allowed for the facile, rapid synthesis of short peptides in a one bead/one sequence manner. This approach allows for screening peptide libraries for binding and catalytic activities. Once hits are found, the sequence of the active …
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Studies on Solid-Phase Peptide Synthesis: The Synthesis of Glucagon
<p>The feasibility of the stepwise-solid phase approach was studied in the synthesis of the peptide hormone glucagon. The choice of this molecule was based upon: (1) the unusual chemical structure; (2) biological significance. The main features of the strategy employed are: (1) the use of …
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Design and synthesis of handles for solid-phase peptide synthesis and convergent peptide synthesis
The recent popularity of methods for solid-phase peptide synthesis that use the 9-fluorenylmethyloxycarbonyl (Fmoc) group for N<sup>α</sup>-amino protection has created a need for compatible anchoring linkages and handles. In an effort to develop mild new methods for use in solid-phase peptide …
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Solid phase peptide synthesis and TLR-5 activity analysis of pertide fragments from the Salmonella muenchen flagellin protein
… this protein are suggested to activate TLR-5: Peptide-N1, LQRVRELAVQ; Peptide-N2, LAVQSANGTNSQSD; and Peptide-C1, QNRFNSAITNLGNT. Here, we report the synthesis of these peptides and their activity against TLR-5 expressing HEK-293 cells. Our goal was to resolve the minimal region of flagellin …
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Automated flow peptide synthesis
… by Merrifield nearly sixty years ago, batch solid phase peptide synthesis remains slow at minutes to hours per residue. Here we report a fully automated, flow based approach to solid phase polypeptide synthesis with amide bond formation in seven seconds and total synthesis times of forty …
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Active Insulin Analogs That Do Not Require Refrigeration
… process, and thus do not require refrigeration. Solid phase peptide synthesis techniques and the incorporation of backbone modified amino acids into peptide sequences will be used to design novel insulin analogs.
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Synthesis of aromatic peptide nucleic acids
The synthesis of two analogues of Aromatic Peptide Nucleic Acids (APNAs) monomers was successfully achieved. These Peptide Nucleic Acid analogues could be used as building blocks for the synthesis of oligomers with the DNA and RNA recognition properties of PNAs in addition to a more hydrophobic …
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A bioactive peptide from the skin of the Chinese Torrent Frog, <i>Amolops wuyiensis</i>
Peptides derived from frog skin secretion have great potential as drug leads as they exhibit many bioactive functions. In this study, the precursor peptide of Lividin-AW was identified from the skin secretion of the Chinese torrent frog (Amolops wuyiensis) by using ‘shotgun’ cloning and its encoded …
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Deciphering the mechanism by which humanin binding to IGFBP3 inhibits neuronal cell death in alzheimer’s disease
… in Alzheimer’s disease (AD). The neuroprotective peptide humanin (HN) counteracts this IGFBP3-induced cell death. Unfortunately, the natural synthesis of this peptide decreases with age coincident with the time that people are likely to develop AD. In addition, the precise mechanism of action of …
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Discovery and functional study of a bioactive peptide QUB-1892 from the defensive skin secretion of The Edible Frog (Pelophylax eculentus)
… kl esculentus and was named QUB-1892. The mature peptide was synthesised by solid-phase peptide synthesis (SPPS). RP-HPLC and MALDI-TOF MS were used to purify and analyse the mature peptide. Its minimal inhibitory concentration (MIC) was obtained by using different micro-organisms and its …
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Bioactive evaluations of novel peptide QUB-2552 from the skin secretion of the Chinese Large Odorous frog, Rana (Odorrana) livida
… skin secretions are rich resources of bioactive peptides, some of which could be developed as drug candidates. Among these natural peptides, antimicrobial peptides have attracted great attention given that they might help to deal with the problem of antibiotic resistance. In this study, the …
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Identification, isolation and bioactivity assessment of a novel peptide from the skin of Heilongjiang Brown Frog (Rana amurensis)
… substance form skin secretions, various peptides or proteins, was considered as treasures in medical treatment. The mechanism of antimicrobial peptides (AMPs) on antimicrobial activities was different from antibiotics and AMPs exhibit considerable value against antibiotic …
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A bioactive peptide QUB3009 from the defensive skin secretion of the frog, Phyllomedusa tomopterna
… which include biogenic amines, alkaloids, peptides and proteins. Antimicrobial peptides from frog skin secretions display vital broad-spectrum antimicrobial activity and have been promoted for the research of novel chemical drugs.<br/><br/>In this thesis, an antimicrobial peptide from the …
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Using peptides to examine the interaction interface between Aspartate transcarbamoylase and Dihydroorotase in pyrimidine biosynthesis in Aquifex aeolicus
… respectively, in <em>de novo</em> pyrimidine biosynthesis. Both enzymes form an active complex (DAC) in <em>Aquifex aeolicus</em>, where loop A of DHOase interacts with a domain of ATCase. The main objective of this work is to determine the function of specific residues of loop A in DAC …
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The discovery and functional study of a bioactive peptide QUB-1418 from the defensive skin secretion of the Wuyi torrent frog (Amolops wuyiensis)
… scientists have been studying antibacterial peptides (AMPs), which have great potential to be a substitute for antibiotics. <br/><br/>In this study, the cDNA sequence encoding a bioactive peptide named QUB-1418, and it has been identified in the skin secretions of Amolops wuyiensis. The …
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Identification, isolation and bioactivity assessment of a bioactive peptide QUB-1465 from the skin secretion of Hylarana guentheri
… skin secretions are a rich source of bioactive peptides, some of which can be developed as drug candidates. Among these natural peptides, AMPs have attracted much attention because of its potential to replace traditional antibiotics and solve the problem of microbial resistance as AMPs kill …
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Isolation and identification of an antimicrobial peptide with high anticancer activity and selectivity, QUB-2584 from the skin secretion of Litoria caerulea
Large quantities of antimicrobial peptides derived from the frog skin secretion have the potential to be therapeutic drugs due to their bioactivities. Their various and complex structural classification, physical-chemical properties, and mechanism contributing to their bioactivities have been …
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Analysis of a novel antimicrobial peptide QUB-1746 from the skin secretion of the frog, Kassina senegalensis
… such as biogenic amines, steroids, alkaloids, peptides and proteins. In this thesis, the full-length cDNA sequence of a novel antimicrobial peptide, named QUB-1746, was cloned from a Kassina senegalensis skin secretion derived cDNA library through ‘shotgun’ cloning. The mature peptide was …
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The functional evaluation of a bioactive peptide, QUB-1998, discovered from the skin secretion of the frog, Pelophylax nigromaculata
… alternatives. Through decades of investment, peptides have been proven to be one potential group of biologics that may contribute to the development of new antibacterial drugs, as they combine the advantages of both small molecule medicines and therapeutic proteins.<br/>Through molecular …
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A bioactive peptide QUB2177 from the defensive skin secretion of the frog, <i>Odorrana livida</i>
… to be unique sources of biologically-active peptides. It has been generally approved that the defensive peptides derived from amphibian skin secretions could be grouped into three broad types in accordance with their established biological effects, including antimicrobials, protease …
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