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Showing 1 to 20 of 29 for “"snRNPs"”.

  1. Purification and crystallization of spliceosomal snRNPs

    … Spleißosoms sind die Ribonukleoproteinpartikel (snRNPs) U1, U2, U4, U5 und U6. Neben der U1 small nuclear RNA (snRNA) enthält das U1 snRNP drei partikel-spezifische Proteine, U1-A, U1-70k und U1-C. Des weiteren findet man einen Satz von sieben Proteinen, den Sm-Proteinen, die auch in anderen …

    goettingen Repository record for Purification and crystallization of spliceosomal snRNPs (opens in a new tab)

  2. Functional and structural investigation of spliceosomal snRNPs

    … kleinen Ribonukleoproteinpartikeln (engl. snRNPs) und nicht-snRNP-Proteinen gebildet. In Hefen und Menschen ist das U2 snRNP eine essentielle Komponente beim Spleißen von pre-mRNA-Molekülen, wobei es während der Bildung des Spleißosoms an der Erkennung bzw. Auswahl der Verzweigungsstellen …

    goettingen Repository record for Functional and structural investigation of spliceosomal snRNPs (opens in a new tab)

  3. Strukturelle und funktionelle Untersuchungen zum m3G-Cap-vermittelten Kernimport spleißosomaler U snRNPs durch Snurportin1

    … der Biogenese spleißosomaler Untereinheiten (U snRNPs) wird die U snRNA nach der Transkription ins Zytoplasma exportiert, wo die Zusammenlagerung mit sieben Sm-Proteinen erfolgt und nachfolgend das 7-Methyl-Guanosin (m7G)-Cap zu einem 2,2,7-Trimethyl-Guanosin (m3G)-Cap umgewandelt wird. Das …

    goettingen Repository record for Strukturelle und funktionelle Untersuchungen zum m3G-Cap-vermittelten Kernimport spleißosomaler U snRNPs durch Snurportin1 (opens in a new tab)

  4. Structural and functional characterization of proteins involved in the biogenesis of spliceosomal U snRNPs

    … small nuclear ribonucleoprotein particles, UsnRNPs). Die Biogenese der UsnRNPs schließt in höheren Eukaryoten einen nukleo-zytoplasmatischen Transportzyklus ein. Das Ziel der vorliegenden Arbeit bestand in der strukturellen sowie funktionellen Charakterisierung zweier verschiedener Proteine …

    goettingen Repository record for Structural and functional characterization of proteins involved in the biogenesis of spliceosomal U snRNPs (opens in a new tab)

  5. Electron microscopic localization of tagged proteins in the yeast S. cerevisiae spliceosomal U4/U6.U5 trisnRNP

    … den sogenannten U1, U2, U5 und U4/U6 snRNPs sowie zahlreichen nicht-snRNP Spleißfaktoren assembliert. Die snRNPs bestehen aus einem RNA-Molekül (bzw. zwei weitgehend basengepaarten RNAs im Fall von U4/U6) und mehreren Proteinkomponenten. Die Assemblierung des Spleißosoms beginnt mit …

    goettingen Repository record for Electron microscopic localization of tagged proteins in the yeast S. cerevisiae spliceosomal U4/U6.U5 trisnRNP (opens in a new tab)

  6. Spliceosomal small nuclear ribonucleoprotein particle trafficking and maturation in the nuclear compartment

    … snRNAs, in the context of functional snRNPs, are targeted to elongating pre-mRNAs. This finding presents us with a novel assay with which to dissect the mechanism by which snRNPs are targeted to nascent pre-mRNA transcripts. Two critical advantages offered by this system are …

    uiuc Repository record for Spliceosomal small nuclear ribonucleoprotein particle trafficking and maturation in the nuclear compartment (opens in a new tab)

  7. Investigation of Protein-protein Interactions within the Human Spliceosomal U4/U6.U5 tri-snRNP Particle

    … Proteinen des U5, wie auch des U4/U6 snRNPs. Es kann daher als potentiell brückenbildendes oder vernetzendes Protein bei der Formation oder der strukturellen Stabilisierung des tri-snRNPs gesehen werden. Die stabile Integration des 102K Proteins in das U5 snRNP wird über Interaktionen …

    goettingen Repository record for Investigation of Protein-protein Interactions within the Human Spliceosomal U4/U6.U5 tri-snRNP Particle (opens in a new tab)

  8. Biogenese der spleißosomalen UsnRNPs

    … von RNP-Untereinheiten des Spleißosoms - den U snRNPs und nährten den Verdacht, dass diese Proteine Hilfsfaktoren der U snRNP-Biogenese sein könnten. Das Ziel der vorliegenden Arbeit war daher, mechanistische Details über die Zusammenlagerung von U snRNPs in vivo zu ermitteln und die Rolle von …

    lmu-germany Repository record for Biogenese der spleißosomalen UsnRNPs (opens in a new tab)

  9. Molecular analysis of wheat snRNAs

    … mediated by small ribonucleoprotein particles (snRNPs), formed from small nuclear RNAs (snRNAs) and their associated nuclear proteins.

    uiuc Repository record for Molecular analysis of wheat snRNAs (opens in a new tab)

  10. Identificação de parceiros de interação para a cinease reguladora de splicing SRPK2

    … uma cinase reguladora de fatores de splicing não-snRNPs (proteínas SR), e de fatores snRNPs (U4⁄U6-U5 tri-snRNP). SRPK2 regula as proteínas SR fosforilando-as em seus resíduos de serina e recrutando- as para a formação do spliceossomo, enquanto atua na formação do complexo U4⁄U6- U5 tri-snRNP, …

    brazil-ufv Repository record for Identificação de parceiros de interação para a cinease reguladora de splicing SRPK2 (opens in a new tab)

  11. Investigation of the higher order structure of the spliceosomal RNA network

    … räumliche Anordnung der beteiligten U1 und U2 snRNPs in frühen spleißosomalen Komplexen ist wenig bekannt. Beide snRNPs, zusammen mit weiteren nicht-snRNP Proteinen, sind in der korrekten Positionierung der für das Spleißen wichtigen funktionellen Bereiche der prä-mRNA involviert. Um die …

    goettingen Repository record for Investigation of the higher order structure of the spliceosomal RNA network (opens in a new tab)

  12. Localization of Small-Nuclear-Rna Genes of Drosophila Melanogaster on Polytene Chromosomes

    … with hnRNA and small nuclear ribonucleoproteins (snRNPs) act as antigens that are precipitated by antibodies from patients with lupus erythematosus, an auto-immune rheumatic disease, have sparked new interest in these molecules. snRNAs have been found to be highly conserved in evolution and …

    uiuc Repository record for Localization of Small-Nuclear-Rna Genes of Drosophila Melanogaster on Polytene Chromosomes (opens in a new tab)

  13. Crystal structure of a human U5 snRNP specific binary complex and crystal structure of a histone deacetylase-like bacterial amidohydrolase

    … Hefe als auch beim Menschen Bestandteil der U5 snRNPs, welche am Spleißen von mRNAs beteiligt sind. Weiterhin spielt U5-52K als CD2 Rezeptor-Bindeprotein 2 (CD2BP2) eine Rolle bei der Immunantwort, da es mit seiner GYF-Domäne spezifisch an ein prolinreiches Sequenzmotiv der zytoplasmatischen …

    goettingen Repository record for Crystal structure of a human U5 snRNP specific binary complex and crystal structure of a histone deacetylase-like bacterial amidohydrolase (opens in a new tab)

  14. Elektronenmikroskopische 3D Strukturbestimmung des Spleißosoms

    … der prä-mRNA jeweils neu aus den fünf snRNPs (`small nuclear ribonulceoprotein particles´) und weiteren Faktoren aufgebaut. Der spleißosomale Komplex A, bestehend aus U1 und U2 snRNP, verbindet sich mit dem U4/U6.U5 tri-snRNP, einem Komplex der U4, U5, U6 snRNPs, um den …

    goettingen Repository record for Elektronenmikroskopische 3D Strukturbestimmung des Spleißosoms (opens in a new tab)

  15. Structural studies of spliceosome assembly and catalysis

    … its component small nuclear ribonucleoproteins (snRNPs) and protein factors. A series of conformational and compositional changes produces the catalytically active spliceosome, in which the snRNA components of the snRNPs mediate intron recognition and catalysis of splicing. Despite the importance …

    cambridge Repository record for Structural studies of spliceosome assembly and catalysis (opens in a new tab)

  16. Examine the role of minor splicing pathway in spinal muscular atrophy

    … in the levels of minor splicing (U12 intron) snRNPs in SMA mice and a restoration of a U12 intron-containing gene partially rescued disease phenotypes in SMA animal models. Here we utilized viral delivery system to investigate the potential effect of increasing minor splicing snRNAs on SMN …

    missouri Repository record for Examine the role of minor splicing pathway in spinal muscular atrophy (opens in a new tab)

  17. Molecular analysis of peasnRNA variants

    … and may result in the formation of unique snRNPs during development.

    uiuc Repository record for Molecular analysis of peasnRNA variants (opens in a new tab)

  18. Identification of Critical Residues in Spliceosomal Protein Dib1

    … spliceosome. The spliceosome is composed of five snRNPs (U1, U2, U4, U5, and U6) and many associated proteins which help orient the pre-mRNA in such a way that catalyzes the splicing reactions. Of the associated proteins, Dib1 in S. cerevisiae is amongst those whose biochemical function still …

    tdl Repository record for Identification of Critical Residues in Spliceosomal Protein Dib1 (opens in a new tab)

  19. Biochemical and Functional Characterization of Novel RNA-binding Proteins Interacting with SMN in Motor Neuron-derived Cells

    … spliceosomal small nuclear ribonucleoproteins (snRNPs), it remains a crucial question in the field to gain a better understanding of what specific/distinct function(s) SMN might have in motor neurons. We have identified novel interactions between SMN and two RNA-binding proteins (RBPs) known to …

    ottawa-retro Repository record for Biochemical and Functional Characterization of Novel RNA-binding Proteins Interacting with SMN in Motor Neuron-derived Cells (opens in a new tab)

  20. HUMAN GENETIC VARIANTS IN ESSENTIAL SPLICING FACTORS AND THEIR IMPACT ON IMMUNE PATHOLOGY

    … of five small nuclear ribonucleoproteins (snRNPs) and hundreds of proteins that are generally referred to as splicing factors (SFs). Here we present two unique cases in which genetic variations in two essential SFs, DExD-box polypeptide 39B (DDX39B) and U2 small nuclear RNA auxiliary factor …

    utmb Repository record for HUMAN GENETIC VARIANTS IN ESSENTIAL SPLICING FACTORS AND THEIR IMPACT ON IMMUNE PATHOLOGY (opens in a new tab)

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