Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"snRNAseq"”.
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Myc and Cyclin T1 co-expression as a therapeutic strategy for cardiac repair following myocardial infarction
… Whole-heart single-nuclei RNA sequencing (snRNAseq), in vivo experiments, histology methods, and molecular biology techniques were used to assess Myc and Ccnt1 induced cardiomyocyte-specific gene expression changes, validate cardiomyocyte cell cycle progression, as well as assess the effect …
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Deep Molecular Characterization of Human Cortical Cell-Types
… in the course of this work, we conducted RNAseq, snRNAseq, ATACseq, as well as CAG expansion assays on hundreds of cell-type specific populations and these data, obtained from several different regions of the human neocortex, will be presented in this work. As many neurological diseases are known …
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Identification and characterisation of Pu.1-driven gene regulatory complexes and networks in Acute Myeloid Leukaemia.
… compared and contrasted; Multiomic data (sc + snRNAseq & snATACseq), integrative Bioinformatic analysis (SCENIC+), qPLEX-RIME mass spectrometry analysis, proximity ligation analysis (PLA) to verify leukaemia-specific/selective interactors, co-binding by CUT&RUN analysis and functional …
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Modelling human liver bud development in vitro
… (ACE2+MUC13+). By performing extensive snRNAseq analyses across HepBud formation, I was able to validate that hepatic specification in HepBuds follows a natural developmental path forming hepatoblast which strongly resemble 5/6pcw human foetal hepatoblasts. Moreover, by examining …
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Profiling the dynamics of the active transcriptome in the juvenile and adult brain using spatial transcriptomics
… cell types, the Visium data was integrated with snRNAseq data using the cell2location method. In situ hybridization chain reaction was used to validate the identified gene expression patterns. Fifty-four cell types, annotated using the current published human temporal lobe cell atlas, were …
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The molecular, hormonal and symptomatic responses to hypoglycaemia in mice
… I aimed to use single-nucleus RNA sequencing (snRNAseq) to reveal the hypothalamic molecular signatures and gene expression profiles of different blood glucose levels. Hyperinsulinaemic clamps were used to create carefully controlled hypoglycaemic, euglycaemic and hyperglycaemic experimental …
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The Role of the Circadian Clock in the Paraventricular Nucleus of the Hypothalamus in Diurnal Energy Balance
<p>Circadian rhythms are internal biological rhythms that drive oscillations in physiology and metabolism. Maintained at the level of individual cells throughout the body, the circadian clock is necessary for driving tissue-specific temporal programs of energy balance. Though loss of the circadian …
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Molecular Mechanisms of Opioid Use Disorder in Human Brain Models
<p>Opioid use disorder (OUD) is a national and global public health crisis with no end in sight. While studies from animal models hint at widespread epigenetic and transcriptomic alterations of opioid drugs, the molecular consequences of long-term exposure to opioid drugs in human brain is still …