Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"smooth muscle actin"”.
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Expression of alpha-smooth muscle actin and contraction of collagen-glycosaminoglycan scaffolds by cells derived from canine synovium
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2003.
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Determining alpha-smooth muscle actin expression in embryonic and mesenchymal stem cells of assorted mammals seeded in collagen scaffolds in vitro
… regeneration but the mechanism is unknown. Alpha-smooth muscle actin ([alpha]-SMA) is the protein responsible for contraction, thus determining if it is present in embryos which heal by regeneration will further our knowledge about the causes of regenerative healing. This thesis experimentally …
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Microfluidic platforms to evaluate the effects of drugs on fertility and proteins on cancer progression – a focus on cisplatin, alpha-smooth muscle actin, and glial cell line-derived neurotrophic factor
The introduction of in vitro systems that can mimic organ physiology and allow studying the progression of diseases such as cancer is necessary to get reliable information about cell responses and test drug efficacy and toxicity. Classical cell culture systems facilitate a wide range of clinical in …
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Mechanical Deformation of the Extracellular Matrix Mediates Cellular Activation and Mechanotransduction
… 1 integrin, and the contractile protein, &alpha-smooth muscle actin (&alpha-SMA) . We report that mechanical loading induces RBL-2H3 degranulation via RGD integrins and regulates age-dependent differences in collagen I and III gene expression in neonatal and adult fibroblasts. We also report that …
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Characterization of the Mechanism of PPARγ-Mediated Neointima Formation in Rodents
… PPARγ knockout mice targeted to vascular smooth muscle cells, macrophages, and endothelial cells. The neointima was quantified and also stained for CD31, CD68, CD11b, and "-smooth muscle actin markers. In LPA1, LPA2, LPA1&2 GPCR knockouts, Mx1Cre transgenic, PPARγ fl/- , and uninduced …
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The behavior of rotator cuff tendon cells in three-dimensional culture
… lubrication. Studies have shown that a smooth muscle actin isoform (SMA), which plays a role in the contraction of smooth muscle cells, is expressed in the rotator cuff tendon cells. Previous investigations have been conducted to study PRG4 expression and distribution in different …
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Control of flow and oxygen in a 3-D perfused micro-environment fosters balanced survival of hepatocyte-non-parenchymal cell co-cultures
… with activation of stellate cells (alpha-smooth muscle actin staining).
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Characterization and modeling of cell activation upon TGF-β1 stimulation in a 3-D culture system
… and expression of a marker protein, alpha-smooth muscle actin (α-SMA). The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the …
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Fibronectin splice isoforms in wound healing and vascular development
… the expression of the differentiation marker a-smooth muscle actin which is expressed by myofibroblasts during wound healing and by peri-endothelial cells during blood vessel development.
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Use of fourier transform- infrared spectroscopic imaging as a tool for understanding early molecular events driving breast cancer progression
… characterized by the expression of α-smooth muscle actin (α-SMA) protein, are highly contractile compared with those found in normal tissue. Although the fibroblast to myofibroblast phenotypic transition is drastic and involves changes in cell morphology as well as increased expression …
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In vitro pharmacological inhibition of myofibroblast differentiation and force generation in a collagen-GAG matrix
… expression of the contractile filament [alpha]-smooth muscle actin ([alpha]-SMA), a hallmark of the myofibroblast phenotype. NR6WT fibroblasts were seeded into free-floating cylindrical CG matrices (8 mm in diameter, n=6) and treated with serum-containing media supplemented with TGF-[beta]1 (3 …
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The recruitment of stromal cells to the site of tumor formation
Myofibroblasts are an alpha-smooth muscle actin ([alpha]-SMA)-expressing cell type found within human mammary carcinomas, but not in the normal mammary gland. Myofibroblasts can enhance tumor formation by promoting angiogenesis and invasion, and we therefore sought to better understand how …
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Inhibition of myofibroblast contraction
… improve the quality of nerve regeneration. Alpha smooth muscle actin ([alpha]-SMA) is a critical contractile protein. Its expression can be upregulated by the growth factor TGF-[beta]1, and blocked by the pharmacological agent PP2. To investigate whether blocking SMA expression alone can inhibit …
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The role of microvesicles in EMT and tumour microenvironment
… high level of myofibroblasts marker alpha-smooth muscle actin (α-SMA) cytoskeleton protein and FGF. MVs were isolated from the myofibroblasts were enriched with α-SMA protein. Primary fibroblasts were treated with MVs released myofibroblasts expressed high level of α-MSA protein. Elements …
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Immunhistochemische Charakterisierung von neugebildeten Geweben im Bereich von interventionell eingesetzten Okkludern für den Verschluss von angeborenen Septumdefekten des Herzens
… Reifungszellenmarker für glatte Muskelzellen (smooth muscle actin, smooth muscel myosin, h-caldesmon, desmin) identifiziert werden. Im neuformierten Gewebe fanden sich typische immunhistochemische Muster für kleine Gefäße und Kapillare. Inflammatorische Zellen wurden als Makrophagen (CD 68+) …
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The ageing hair follicle environment. Alterations in the female scalp and mesenchyme with age.
… Protease nexin-1 (SERPINE2) increased, while α-smooth muscle actin (aSMA) decreased. Both cell types exhibited elevated cartilage oligomeric protein (COMP) mRNA expression. Proteomics revealed elevated COMP expression in the DF secretome with age, suggesting a more fibrotic phenotype or DS …
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Proteomic comparison of biomaterial implants for regeneration of peripheral nerve tissue
… 3 (TGF [beta]1, [beta]2, and [beta]3) and alpha smooth muscle actin ([aipha] SMA) to measure disparities in proteins associated with wound healing that may determine nonregenerative from regenerative outcomes. Transected nerves treated with silicone or collagen tubes were compared on a "whole …
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Investigation and control of dermal fibroblast signaling during injury repair
… we also discovered and characterized a novel actin cytoskeleton-plasma membrane topology present in human dermal fibroblasts at the extreme end of cellular contractility. We also developed several technical advances: the real-time imaging and manipulation of calcium in 3D microtissues, the …
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Protective Effects of Yuzu and Its Component against Cardiovascular Disease
… activity, myeloperoxidase expression, α-smooth muscle actin expression, and matrix metalloproteinase-2 activity in a permanent LAD occlusion rat model. These results suggest that yuzu and hesperidin have anti-platelet activity, and that intake of yuzu, which contains various flavonoids …
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Role of transcription factor Runx1 in uterine stromal cell differentiation and maternal-fetal interaction during mouse pregnancy
… Furthermore, immunohistochemical analysis of smooth muscle actin and cytokeratin in the uterine sections of the mutant mice revealed a lack of maternal spiral artery modification and restricted trophoblast invasion. Collectively, these studies demonstrated that in the absence of Runx1 the …
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