Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"small molecule ligand"”.
-
Development of pattern recognition technique for classification of RNA secondary structures by small molecules
… first time, revealing guiding principles in RNA:small molecule recognition and supporting our long term goal of classifying unknown RNA conformations and even function. Specifically, an aminoglycoside receptor library was obtained with commercially available aminoglycosides and through simple …
-
DNA Constraints for Rational Control of Macromolecular Conformation
… controls the intramolecular states of other macromolecules are rare. In this thesis, short (10--20 bp) DNA duplexes are used as structural constraints to control RNA conformation. Based on the X-ray crystal structure of the 160-nucleotide P4--P6 RNA domain of the Tetrahymena group I intron, pairs …
-
Engineering GFP-Family Member Protiens to Achieve Novel Functions: A Class of Proteins Limited Only by the Imagination
… fluorescence put under the direct control of a small molecule ligand paving the way for switchable and biosensor GFPs. The more one reads about GFP the more it becomes clear that the applications of this amazing protein family are still expanding at a very rapid pace. Here, I will focus on a few …
-
Investigating the interactions of G-quadruplexes with transcription and chromatin structure
… G4s function to promote transcription, the small molecule ligand pyPDS was used to stabilise G4s in compacted chromatin. This was found to diminish the loss of G4s and mitigated loss of RNA Pol II at promoters upon chromatin compaction, indicating a role of G4s in promoting polymerase …
-
Engineering Estrogen Receptor-Based Gene Switches and a Superoxide Dismutase for Therapeutic Applications
… that bind and respond to two synthetic small molecule ligands---4,4'-dihydroxybenzil (DHB) and 2,4-di(4-hydroxyphenyl)-5ethylthiazole (L9)---with high specificity and potency. The resulting highly specific DHB/7-S receptor and L9/L7-E receptor pairs exhibit nanomolar or subnanomolar …
-
Sequence Effects and Ligand Selectivity in Targeting Non-Canonical DNA Structures
… selectively affect these functions, improved small-molecule ligands are required. We have embarked on the synthesis of one such novel bis(quinolin-3-ylmethylene)-based ligand, which is predicted to be more selective for hybrid-stranded G4 structures. In addition, this ligand is predicted to be …
-
A Pharmacological Strategy Against African Sleeping Sickness
… yeast and humans, we hypothesize there may be a molecule that inhibits the function of<em> </em>tbdUTPase but not of yDut1 and hDUT. The endogenous yDut1 was knocked down via auxin inducible degron system in order to complement its loss by transforming plasmids containing human and <em>T. …
-
Investigating the binding mode of azide labelled derivatives of Hoechst 33258 by NMR, UV-Vis and IR spectroscopy
… a method for studying the dynamics of dsDNA: small molecule ligand association and dissociation through 2D-IR spectroscopy. The strategy employed was to synthesise azide-bearing derivatives of the archetypal minor groove binding ligand, Hoechst 33258, with the azide acting as a reporter …
-
Protein-based Degrader Strategies against Oncogenic RAS
… due to the absence of binding pockets for small-molecule drugs, and the significant challenge of delivering protein-based inhibitors across the cell membrane to reach intracellular RAS. This thesis focuses on the development of novel protein-based degrader strategies against KRAS G12D, …
-
Biochemical and synthetic chemical approaches to studying the biosynthesis and regulation of bacterial secondary metabolites
… has been dedicated to developing bioactive small molecules to treat pathogenic infections. Despite the huge amount of efforts in the past few decades, resistant strains continue to emerge with every introduction of new therapeutic compounds into the clinic, leading to multi-drug resistant …