Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"small molecule interactions"”.
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Investigating Karyopherin B1: small molecule interactions for cancer therapy
… cells. Inhibition of KpnB1 in these cells using small interfering RNA (siRNA) resulted in significant cancer cell death via apoptosis, suggesting KpnB1 is essential for cancer cell survival. Within our laboratory, we established that candidate small molecules targeted against KpnB1 identified …
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High Throughput Screening for Small Molecule Interactions with Nucleic Acid Binding Proteins
… genetic manipulation. To address this challenge, small molecule binding screens were implemented against transcription factor and RNA-binding protein targets. The MYC oncoprotein was successfully inhibited by an indirect strategy through stabilization of its obligate interacting partner MAX in an …
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DEVELOPMENT OF A NEW SCREENING AND DETECTION METHOD FOR IDENTIFYING PROTEIN-SMALL MOLECULE INTERACTIONS
Small molecules are known to play critical role in understanding most biological mechanisms of cells and organisms. Some examples, such as RNAs, peptides and drug molecules, etc., work by modulating cellular function, but with unknown modes. In most cases, these actions involve the small molecule …
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A Study of Polymer-Small Molecule Interactions for Solid-Liquid Two Phase Partitioning Bioreactors With Emphasis on the Bioproduction of 2-Phenylethanol
… target compounds for selected biotransformation molecules including 2-phenylethanol, cis-1,3-indandiol, iso-butanol, succinic acid and 3-hydroxybutyrolactone. Furthermore, the biotransformation from L-phenylalanine to 2-phenyethanol, an important aroma compound in industry, was examined in …
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Structural Studies of Protein-Small Molecule Interactions: Bombyx Mori Pheromone Binding Protein Specificity for Bombykol, and the Substrate Specificity of Pantocin A Biosynthetic Proteins
Interactions between small molecules and proteins mediate the biological processes of life. A knowledge of how proteins specifically interact with small molecules provides us with the tools to probe complex biology, but also allows us to develop potent and specific drugs to manipulate these …
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Protein engineering of botulinum toxins with enhanced ganglioside binding capacity A biophysical and computational approach
… understanding of the molecular nature of these interactions will facilitate the generation of modified BoNT proteins with novel characteristics and significant therapeutic potential. This project was aimed principally at molecular characterisation of the interaction of BoNT/A with gangliosides, …
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Strategies for Computational Protein Design with Application to the Development of a Biomolecular Tool-kit for Single Molecule Protein Sequencing
… remarkable affinities and specificities for small-molecule and peptide binding partners. To improve the success rate of rational, computational protein design and widen the scope of potential applications, it is useful to define generalized strategies and automated methodology to improve …
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Development and Application of Covalent-Labeling Strategies for the Large-Scale Thermodynamic Analysis of Protein Folding and Ligand Binding
… and quantify both known and novel protein-ligand interactions in cell lysates. The ligands under study included the well-known Hsp90 inhibitor geldanamycin and the less well-understood omeprazole sulfide that inhibits liver-stage malaria. In addition to protein-small molecule interactions, …
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Small Molecule Binding to Disordered Proteins
… has arisen from a lack of understanding of how small molecules bind disordered proteins. In this thesis, I combine biophysical experiments and computational ensemble determination techniques (metadynamic metainference) to characterise small molecule interactions with disordered proteins. After …
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Understanding small molecule-protein interactions
The binding of small molecules to a protein is among the most important phenomena in the chemistry of life; the activity and functionality of many proteins depend critically on binding small molecules. A deep understanding of protein-small molecule interactions and the interplay between ligation …
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Strategies for targeting cancer: small molecules, epigenetics and drug design
… cancer; treatments utilizing chemotherapeutics, small molecule interactions, metallocage drug delivery, epigenetics and protein activity inhibition. The first part of my research focused on the significance of Cisplatin as a chemotherapeutic. My findings indicate the unexpected speciation of …
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Proton relaxation studies of the interaction of small molecules with cytochrome P450
… the proton relaxation cam times of a variety of small molecules has been studied. In some cases, Fe-proton distances in the small molecule-protein complex are determined. For water protons the following results are obtained: The substrate (camphor) complex of P450, which is in a mainly high-spin …
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Relating structure and function: Discovery of novel inhibitors of myotonic dystrophy
… unique opportunity to develop sequence-selective small molecule inhibitors that bind to the DNA or RNA and inhibit RNA synthesis or aberrant RNA-protein interactions directly as well as many downstream effects. The motivation behind and progress toward nucleic acid drug targeting is described in …
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Design and Applications of Split-Luciferase Systems in Vitro and in Cellulo
… for the detection of a variety of macromolecular interactions in vitro, in cellulo and in vivo. Spilt-protein reporters, such as split-firefly luciferase (Fluc), depend upon the conditional reassembly of the genetically fragmented enzyme. The two fragments of Fluc conditionally reassemble and …