Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 114 for “"small RNAs"”.
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Modulation of Alphaviruses by Small RNAs
… It is well known that invertebrates rely on small RNA pathways as an adaptive antiviral defense. The canonical antiviral response in these organisms involves dicer enzymes that cleave viral double-stranded RNA replicative intermediates (RIs) into small interfering RNAs (siRNAs; ~21-24 …
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Biogenesis of Y RNA–derived small RNAs
Small non-coding RNAs (sRNAs) constitute a significant portion of the transcriptome in eukaryotes. Many of these sRNAs regulate gene expression. Next-generation sequencing (NGS) has revealed a plethora of previously uncharacterised sRNAs with potential biological function, a number of which …
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Differential expression of small RNAs in soybean
Small RNAs are non-protein coding RNAs that regulate gene expression in plants primarily by cleaving mRNA. Two major classes of small non-coding RNAs are microRNAs (miRNAs) and short interfering RNAs (siRNAs) of 21-24 nucleotides. I selected some small RNAs that show tissue specificity based on …
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The role of small RNAs in C4 photosynthesis
… of the C4 cycle may be targeted by microRNAs (miRNAs). To explore this, a library of sRNA sequences from mature leaf tissue of the model C4 species Cleome gynandra L. was generated and then searched against a list of expressed sequence tag sequences for candidate genes of the C4 cycle. To …
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Small RNAs in Tomato: from defence to development
… As part of the silencing machinery, micro (mi)RNAs orchestrate silencing of their targets, either directly or through cascades of secondary small interfering (si)RNAs. To investigate the role of RNA silencing in plant immunity, I chose to focus on the miR482/2118 family, because of its …
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Small RNAs within the developing seed of Arabidopsis thaliana
… but relatively little is known about the small RNA (sRNA) populations found within the tissues composing the seed. We set out to profile the sRNA complement found within whole seeds and the embryo and endosperm tissues within the seed. In whole seeds, variation in sRNA activity at TEs near …
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Small RNAs Regulate Transcription by Interacting with Noncoding RNA Transcripts
… dependent manner. The mechanism by which RNAs achieve promoter recognition has remained unclear. Sequence specific recognition could be achieved by (1) RNA hybridization to genomic DNA, or (2) RNA recognition of some uncharacterized RNA species. Promoter targeted duplex RNA has been shown …
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Expression and function of small RNAs in normal and psoriatic skin
… mRNA transcript levels in psoriatic skin. microRNAs: miRNAs) are a class of short, regulatory RNAs that play critical roles in human development and disease. I hypothesized that differential expression of miRNAs might underlie some of the gene expression changes in psoriatic skin. Thus, I sought …
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Identification and characterization of novel small RNAs in Desulfovibrio vulgaris Hildenborough
… and culture-based methods to identify novel, small, regulatory RNAs (sRNAs)--an important class of cellular regulators that have yet to be studied in <italic>D. vulgaris</italic>--and to determine their effect on cellular processes within the organism. Computational and transcriptomic …
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Structural variation at soybean loci regulating small RNAs and seed color
… seed color is determined by a specific class of small RNAs known as short- interfering RNAs (siRNAs). The dominant ii allele of the I (inhibitor) locus is composed of an inverted-repeat cluster of six chalcone synthase (CHS) genes on chromosome 8 whose unique arrangement generates CHS siRNAs that …
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A survey of fly and nematode small RNAs by deep sequencing
Small RNAs of -22 nt length play a variety of roles in the biology of animals by repressing the translation or stimulating the degradation of complementary messenger RNAs. Depending on the structure of their precursors, they can be categorized as either microRNAs (miRNAs) or small interfering RNAs …
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Idendification and characterisation of novel small RNAs from repetitve elements in mammals
… latter may indicate a large proportion of these RNAs. Further analysis of the B2 and 55 rRNA repeat-associated short RNA revealed both to have reduce accumulation in Dicer-null embryonic stem cells, implicating a possible association with a known component of the RNAi pathway. Dicer was also …
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Rickettsia prowazekii and its ‘Junk DNA’: The Identification and Characterization of Small RNAs
… bacterial virulence and growth called bacterial small RNAs (sRNAs). These sRNAs are classified as either trans-acting, whose biogenesis is predominantly linked to intergenic regions, or cis-acting, encoded on the antisense strand of an open reading frame (ORF). Despite being present in a majority …
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Roles of Small Rnas and Paralogous Proteins In Bacillus Anthracis Virulence Gene Regulation
… control through the function of small regulatory RNAs (sRNAs). Small regulatory RNAs (sRNAs) are short transcripts that base-pair to mRNA targets or interact with regulatory proteins. sRNA function has been studied extensively in Gram-negative bacteria; comparatively less is known …
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Identification and Characterization of the Hfq protein and small RNAs in Francisella novicida
… protein Hfq and identify potential novel small RNAs in <italic>F. tularensis</italic> subsp. <italic>novicida</italic> strain U112. The Hfq protein is recognized as an important regulatory factor in a variety of cellular processes, including stress resistance and pathogenesis, and has been …
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SYSTEMIC AND TRANSGENERATIONAL REGULATION OF GENE EXPRESSION BY SMALL RNAS IN C. ELEGANS
Development of an organism requires information contained minimally within a single cell. This information is inherited in two forms- the genome sequence and regulatory molecules. Little is understood about the types of the regulatory molecules inherited or the impact of parental experiences on …
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Integration of small RNAs in the control of Salmonella pathogenicity island 1(SPI1)
… and genetic analyses, I identified a number of sRNAs that feed into hilD or hilA translation to contribute to SPI1 expression. Among them, I demonstrate that two oxygen-dependent sRNAs, FnrS and ArcZ, repress hilD translation. Genetically, the sRNAs base pair with hilD mRNA to regulate …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… In this thesis, I investigate the role of small RNAs to control TEs during global waves of DNA demethylation in cellular reprogramming, naïve pluripotency as well as early mammalian development. Following an introduction to the research questions, in chapter 3 I investigate the mechanism …
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Mechanistic and physiological insights into post-transcriptional regulation by small RNAs SgrS and DicF
Base pairing small regulatory RNAs (sRNAs) are important post-transcriptional regulators of gene expression in bacteria. These sRNAs deploy novel mechanisms to regulate mRNA targets leading to various physiological outcomes during stress conditions including, but not limited to, iron starvation, …
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