Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"small RNA (sRNA)"”.
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Understanding the physiological role of the small RNA RydC
… aims to understand the physiological role of the small RNA (sRNA) RydC and the signals controlling RydC production. RydC was previously shown to stabilize cfa (cyclopropane fatty acid synthase) mRNA, resulting in higher levels of cyclopropane fatty acids in the cell membrane. Here, we show that …
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An E. coli small RNA inhibits translation initiation from a distance
In bacterial systems, small RNA (sRNA)-dependent translational repression is commonly carried out via sRNA-mRNA base pairing interactions near the Shine-Dalgarno (SD) region. In this so-called “canonical” mechanism, the sRNA is the direct regulator; it competes with the initiating ribosomes while …
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Small RNAs within the developing seed of Arabidopsis thaliana
… but relatively little is known about the small RNA (sRNA) populations found within the tissues composing the seed. We set out to profile the sRNA complement found within whole seeds and the embryo and endosperm tissues within the seed. In whole seeds, variation in sRNA activity at TEs near …
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INVESTIGATING THE COMPETITION BETWEEN COMPONENTS OF DUAL-FUNCTION RNA
Non-coding RNAs (ncRNAs) and small proteins have both emerged as important regulators of gene expression. Dual-function RNAs encode a small protein and have a separate function as a regulatory RNA. Although first discovered in bacteria, dual-function RNAs have now been identified and characterized …
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Epigenetic Responses of Arabidopsis to Abiotic Stress
… via epigenetic modification (methylation) and small RNA (sRNA). For all of the treatments except shade, the stress was limited in duration, and the plants were allowed to recover until flowering, to identify changes that persist to reproduction. At flowering, DNA for methylation bisulfite …
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Post-Transcriptional Regulation of Virulence Genes by GlmY and GlmZ in Enterohemorrhagic E. Coli
… systems both regulate the transcription of the small RNA (sRNA) glmY. GlmY is known to stabilize another sRNA, GlmZ, which activates the translation of glucosamine synthetase (GlmS) (72). Here we show that GlmY and GlmZ are also important players in the post-transcriptional regulation of …
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Sweet relief: regulating the response to glucose phosphate stress by the small RNA SgrS & Co.
… (i.e., phosphosugar stress) by producing a small RNA (sRNA) regulator, SgrS, which represses translation of sugar transporter mRNAs (ptsG and manXYZ) and enhances translation of a sugar phosphatase mRNA (yigL). Despite a molecular understanding of individual target regulation by SgrS, …
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MicroRNAs and Trans-acting siRNA pathways in Apple (Malus x domestica Borkh.) and Peach (Prunus persica)
The unveiling of small RNA (sRNA)-mediated gene regulatory pathways has profoundly shaped our understanding of the complexity of gene regulation. In eukaryotes, sRNAs have been found to control cellular metabolism, growth and differentiation, to maintain genome integrity, and to combat viruses and …
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Molecular analysis of small RNA and small protein regulation of Escherichia coli stress responses
Small RNA (sRNA) regulators control gene expression throughout all domains of life. In bacteria, they typically affect virulence, metabolism, and stress response genes posttranscriptionally through imperfect antisense pairing with their mRNAs. While most sRNAs are non-coding, a small number act as …
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New Methods of DNA Assembly, Gene Regulation with a Synthetic sRNA, and Cyanobacterium Phenotype Monitoring with Raman Spectroscopy
… traditional techniques. Then, novel synthetic small RNA (sRNA) regulators were designed in a cell-free-system (in vitro) in order to modulate protein expression in biosynthetic pathways. The ability of the sRNAs to regulate mRNA expression with statistical significance was demonstrated. Up to …
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The Functional Characterization of the LysR-Type Transcriptional Regulator QseD and the SorC-Type Transcriptional Regulator LsrR in Enterohemorrhagic Escherichia coli
… these results show that QseD regulates alternate targets in EHEC and K-12 E. coli, and that EHEC O157:H7 has evolved to encode a truncated form of this protein. We also studied the role of the LsrR regulon in EHEC pathogenesis and environmental persistence through biofilm formation. LsrR, a …
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Regulation of the Mannose PTS Operon by the small RNA SgrS
A number of bacterial small RNAs (sRNAs) act as global regulators of stress responses by controlling expression of multiple genes. The sRNA SgrS is expressed in response to glucose-phosphate stress, a condition associated with disruption of glycolytic flux and accumulation of sugar-phosphates. SgrS …
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Identification and characterization of novel small RNAs in Desulfovibrio vulgaris Hildenborough
… and culture-based methods to identify novel, small, regulatory RNAs (sRNAs)--an important class of cellular regulators that have yet to be studied in <italic>D. vulgaris</italic>--and to determine their effect on cellular processes within the organism. Computational and transcriptomic …