Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"smFISH"”.
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Single molecule techniques to probe decision-making processes in developmental biology
… RNA fluorescent in situ hybridization technique (smFISH) to study gene expression in the inner ear and perform allele-specific detection of the X chromosome in mESCs. We used previously-published smFISH procedures as our initial template for investigating biological signaling phenomena in these …
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Microfluidic-based Tools to Investigate Gene Expression and Regulation with Tissue-specificity during Aging in Caenorhabditis elegans
… molecule fluorescence in situ hybridization (smFISH) in adult C. elegans using microfluidics. In Aim 2, I create two complimentary platforms to enable multiple cycles of smFISH, directly addressing the gene-throughput limitations. The first device is an electrokinetic/microfluidic hybrid …
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Investigating the competing endogenous RNA hypothesis genome-wide and in single cells
… combine RNA sequencing and single-molecule FISH (smFISH) approaches to both measure the magnitude of the crosstalk effect genome-wide by perturbing three known ceRNAs (Pten, Vapa, Cnot6l) and to identify mechanisms by which the crosstalk effect acts. We identify hundreds of putative ceRNAs and …
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Optimisation and validation of tools for in situ visualisation of heteroplasmic mtDNA variants.
… fluorescent in situ hybridisation (smFISH) combined with hybridisation chain reaction (HCR) for detection of mtDNA. Taking advantage of Drosophila heteroplasmic strains, we have applied this in vivo in the developing larval brain. Our findings revealed extensive cell-to-cell …
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Quantitative spatial transcriptomics of the developing brain
… fluorescence in situ hybridisation (smFISH), automated confocal imaging and analytical tools to derive ‘Quantitative Spatial Transcriptomics (QST)’ for the spatiotemporal characterisation of gene expression across the developing mammalian forebrain. My thesis research has focused in …
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Highly Constrained Kinetic Models for Quantitative Single-Cell Gene Expression Analysis
… Fluorescence in situ Hybridization (smFISH) to quantify mRNA levels, alongside live-cell imaging to measure TF dwell time and transcriptional bursting kinetics. By integrating these methods into a computational model, we aim to simulate transcriptional dynamics and assess existing …
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Microglial succinate receptor 1 (SUCNR1) sustains chronic CNS inflammation
… fluorescence in situ hybridization (smFISH) and histopathology analysis of people with progressive MS confirmed a predominant expression of SUCNR1 in chronic active and active white matter lesions. When EAE was induced in a constitutive Sucnr1 knockout (Sucnr1-/-) mice, I found that …
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Investigating lymphocyte development in the foetal thymus and modulation by the melanocortin 5 receptor
… RNA fluorescence in situ hybridisation (smFISH) was performed on E17.5 foetal thymus to visually identify gene expression of 100 genes with single-cell resolution. This highlighted potential foetal thymus niche factors that may support innate lymphoid cell development. In the periphery …
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Studying Myeloid Cell Heterogeneity After Spinal Cord Injury via Time-Resolved Single-Cell RNA Sequencing
… fluorescent in situ hybridization (smFISH) and immunofluorescence (IF). A key observation was the persistence of a pro-inflammatory foam cell-like state in both microglia and macrophages, which may contribute to the non-resolving chronic injury. Future studies might investigate the …
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Single molecule investigation of antiviral signaling
… fluorescence in situ hybridization (smFISH) to study viral and antiviral gene expression in individual cells. We precisely quantified kinetics of mRNA expression of RIG-I with respect to IFN- in cells upon infection with Sendai virus (SeV). We found that RIG-I mRNA is directly …
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Interpreting Notch signals: characterisation of dynamic transcription hubs by live imaging
… fluorescence in situ hybridization (smFISH) was performed to detect RNA transcripts. Surprisingly, active transcription of Notch targets was detected in only a third of Notch ON nuclei despite the presence of Mastermind transcription hubs in all nuclei. The low proportion of actively …
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Non-Coding RNA Features Critical to the Replication of HIV-1
… observed replication defects. qPCR analysis and smFISH microscopy revealed that the addition of CG dinucleotides to HIV-1 resulted in a depletion of the cytoplasmic mRNA molecules where the CG-dinucleotides were encoded as exons. A targeted siRNA screen for proteins that destabilize cytoplasmic …
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Quantifying transcriptional regulation in Escherichia coli and phage lambda at single-cell, single-molecule resolution
The information that instructs cells on how to create the molecules that perform cellular functions resides within the DNA. The basic unit of genetic information is called a gene, which is a region of the DNA that contains the instructions to produce messenger RNAs (mRNAs). An enzyme called RNA …