Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"sliding clamp"”.
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Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
… product (∼7.2 kb) in the presence of the sliding clamp. A PCNA-interacting peptide (PIP) box located at the C-terminus of MacDinB was shown to facilitate interaction with the sliding clamp. Furthermore, individual mutations at different positions in the PIP-box led to identification of …
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Molecular and Biochemical Analyses of a Three-Subunit Euryarchaeal Clamp Loader Complex
DNA polymerase requires two processing factors, sliding clamp and clamp loader, to direct rapid and accurate DNA replication. In eukaryotes, the ring-shaped sliding clamp, proliferating cell nuclear antigen (PCNA), encircles double-stranded DNA within its central hole and tethers the DNA …
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Structural Studies of DNA Replication Proteins by X-Ray Crystallography and Molecular Dynamics
… activities. The second functional complex is the sliding clamp which adopts a ring-shaped structure, composed of either two or three protomers. It confers processivity onto the polymerase subunit by tethering it to the template. The third complex is the clamp loader complex, which loads the …
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Replication-associated base excision repair Of oxidized bases in the mammalian genome
… the stress responsive Rad9-Rad1-Hus1 (9-1-1) DNA sliding clamp. We mapped the overlapping binding sites for all of these interacting protein partners to a small disordered region near the unconserved C-terminus of NEIL1 that is dispensable for its enzymatic activity. In support of the biological …
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Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis
… GINS heterotetramer (Kubota et al., 2003), a ""sliding clamp"" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in …
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Establishing DNA combing to investigate DNA replication stress in vitro
… helicase MCM2-7 and the DNA polymerase sliding clamp PCNA. These data suggest that Ap4A can inhibit the firing of replication origins through disruption of replication complex assembly. Finally, DNA combing is established to measure replication parameters. Here we find that the …
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Single -Molecule and Ensemble Fluorescence Studies of Single -Stranded DNA and Replication Proteins
… single-stranded DNA binding proteins, and the sliding clamp and clamp loader proteins. Fluorescence studies have been widely used to study the properties of biomolecules and here we will use both Fluorescence Polarization Anisotropy (FPA) and Fluorescence Resonance Energy Transfer (FRET) to …
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Clamp loading on the leading strand during DNA replication and initial reconstitution of sister chromatid cohesion establishment
… factors. One such factor is the sliding clamp PCNA, which acts as both a processivity factor to support fast and efficient DNA synthesis and as a recruitment platform in various genomic stability maintenance processes downstream of DNA replication. Regulation of chromatin bound …
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Structural Studies of Three Factors That Affect the Prokaryotic Transcription Cycle; Microcin J25, LAMBDA Q and T4 GP33
… interaction between Gp33 and the DNA-bound sliding clamp of the T4 DNA replisome, Gp45. We obtained crystals of Gp33 bound to the Ec Beta flap that diffracted to 3.0 Ångstroms. The preliminary structure shows Gp33 as a mainly helical protein that wraps around the Beta flap.</p>
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Toxin-antitoxin systems in bacteria : targets, mechanisms, and specificity
… progress through an interaction with the sliding clamp, thus expanding the number of known cellular targets for TA systems. For my second project, I studied interaction specificity in the ParDE TA family. I found that toxins and antitoxins in this family exhibit a strong preference for …
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Charakterisierung des Replikationsproteins ORF904 des archaealen Plasmids pRN1
… PCNA1 beobachtet, welches Teil der archaealen sliding clamp ist und mit ORF80, welches ebenfalls auf pRN1 kodiert ist. Für die Interaktion von ORF904 mit diesen Proteinen war die Anwesenheit von ATP und einem Oligodesoxynukleotid notwendig. Die Aminosäuren 371-526 von ORF904 wurden als Ort der …
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Epic Search for the Perfect Partner: MutSgamma Promotes Efficient Meiotic Recombination and Homolog Pairing in Mouse Spermatocytes
… crossover. It is thought that MutSgamma forms a sliding clamp to encircle crossover-specific intermediates in order to stabilize and protect these intermediates from dissolution.</p> <p>As mentioned, interhomolog recombination and pairing are mutually dependent upon each other. However, which …
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Making Molecular Movies: Using Single-Molecule Techniques to Unveil Hidden Features of Protein-Chromatin Interactions
… specifically the dynamics of the eukaryotic sliding clamp, proliferating nuclear cell antigen (PCNA). PCNA is an essential ring-shaped protein required for replication in addition to diverse cellular processes including DNA repair, chromatin maintenance, and sister chromatid cohesion. To …