Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"slice cultures"”.
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Modelling neuroimmune interactions using organotypic slice cultures
… using the model of organotypic hippocampal slice cultures; an in vitro model that overcomes the previously mentioned obstacles. The study sought to expound on immunological and electrophysiological responses to the infection. A viable and moderate BCG infection was established in the …
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Revealing Mechanisms of Sporadic Alzheimer’s Disease by Modelling in Organotypic Hippocampal Slice Cultures
… risk factors with AD. Organotypic hippocampal slice cultures (OHSCs) are an underused tool in dementia research. They contain a variety of cell types including neurons, astrocytes, microglia, and oligodendrocytes, and maintain some of the structure and connections of the hippocampus. Yet, slice …
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Characterization and development of a stroke-induced model of acquired epilepsy in organotypic hippocampal slice cultures: role of the cannabinoid CB1 receptors in modulation of neuronal excitation and inhibition
… initiated to develop an organotypic hippocampal slice culture model of acquired epilepsy and examine the changes in distribution and function of the endogenous CB1 receptor system. We utilized 4-aminopyridine and glutamate to induce separate excitotoxic injuries to slice cultures. Both injuries …
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Investigating gene expression differences in acute and organotypic mouse hippocampal brain slices as models for studying the brain
Organotypic brain slice cultures (OBSCs) are widely used to study neural circuit function, but their molecular and cellular composition relative to acute brain slices remains poorly characterized. Here, using single-nucleus RNA sequencing, I performed a comprehensive comparison of gene expression …
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Use of an Atg16l1 mouse model to study the role played by autophagy in neuroprotection
… on virus proliferation in organotypic brain slice cultures while viral nsP1 and capsid expression was reduced in Atg16l1 KO embryonic fibroblast cultures. Semliki Forest Virus markers did not colocalize with autophagosomes in autophagy competent cells. While Semliki Forest Virus causes …
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Regulation of Astrocyte Functions by a Parkinson's Disease Gene, DJ-1
… quantification 18 8. Organotypic cortical slice cultures 18 9. Cell culture 19 10. Western blot analysis . 19 11. Immunoprecipitation 21 12. Quantitative real-time PCR. 21 13. Reverse transcriptase PCR 22 14. DNA constructs . 23 15. Transfection 23 16. Proximity ligation assay (PLA) 23 17. …
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Propofol: Neuroprotektion in einem In-vitro-Modell des traumatischen Hirnschadens
… laboratory study organotypic hippocampal brain-slice cultures were gained from six- to eight-day-old mice pups. After 14 days in culture, hippocampal brain slices were subjected to a focal mechanical trauma and subsequently treated with different molar concentrations of propofol under both …
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A Novel Link Between Oxidative Stress Proteins and Estrogen Receptor Alpha-Mediated Gene Expression
… cells. Additional characterization using brain slice cultures suggests a protective role for E2 and SOD1 in the cerebral cortex. These combined studies highlight a novel link between oxidative stress proteins and ERalpha-mediated gene expression in target tissues.
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Magnetic Resonance Imaging in the Assessment of Anti Prion Mediated Neurotoxicity In Vivo
… As previously found in cerebellar organotypic slice cultures (COCS) antiprion mediated neurotoxicity was found to be target and eptiopic specific. Neuronal expression of PrPC is sufficient for lesion induction and lesion induction is independent from cross-linking. Further I investigated …
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Establishing in vitro models of neuroinflammation to investigate neuroimmune responses in neurocysticercosis
… establish the rodent-derived organotypic brain slice culture (OBSC) system as an in vitro model for investigating neuroinflammatory signalling and activation of microglia and astrocytes in the brain. To validate this model of neuroinflammation, OBSCs from neonatal mice were treated with the …
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Brain Inflammation Regulated by PINK1
… the roles of PINK1 in brain inflammation using slice cultures prepared from PINK1 knockout (KO) and wild type (WT) mice since slicing mimics injury. For up to 24 h after slicing, mRNA expression levels of pro-inflammatory cytokines (TNF-a, IL-1b, and IL-6) measured at 6 to 24h were higher in KO …
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DJ-1, a Parkinson’s Disease-associated Gene, Prevent Excessive Glial Inflammatory Responses through Interaction with SHP-1
… on IFN-g. Similar results were obtained in brain slice cultures, where IFN-g induced much stronger STAT1 phosphorylation and inflammatory responses in KO slices than in WT slices. Moreover, IFN-g treatment induced neuronal damage in KO slices. Collectively, these findings suggest that DJ-1 may …
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Elucidating Astrocytic Diversity in Human Cerebral Organoids
… overcome these difficulties, cortical organoid slice cultures derived from human embryonic stem cells (hESCs) were generated. The potential for their long-term growth permits the generation of large numbers of glial cells and allowed for a longitudinal assay of astrocytic diversity. This thesis …
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Endoplasmic Reticulum Dynamic Structural Changes in Neurons: The Fission-Fusion Phenomena
… Ca2+ entry to the cell in murine primary cultures and hippocampal slice cultures. Using different pharmacological approaches, we demonstrate, that ER fission is triggered independently on Ca2+ from ER stores. Subsequently, we show that mild hypothermia, reported to be protective in …
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Role of Aryl Hydrocarbon Receptor in Circadian Rhythm: A Novel Pathway to Dioxin Toxicity
… by preincubation with FICZ in in vitro SCN slice cultures. Lastly, potential effects of TCDD on circadian rhythm were examined at the molecular and behavioral level. Exposure to TCDD affected circadian expression of clock genes and attenuated the light-induced phase shifts. Taken together, …
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The Mechanisms of Tau Entry to the Cytosol
… of tau in 2D neurons and 3D organotypic slice cultures. Conversely, cholesterol supplementation or overexpression of the cholesterol transporter protein NPC1 reduced entry and almost completely blocked seeded aggregation. My findings establish entry to the cytosol as rate-limiting to …
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Exploring the effects of classical immune activation on circuit excitability and cell viability in the mouse brain
… Using the hippocampal organotypic brain slice culture model in mouse brains, I investigated the effects of classical immune activation on circuit excitability and cell viability. To initiate inflammation, I administered lipopolysaccharide (LPS), an endotoxin derived from gramnegative …
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Role of the PI-3K/Akt and ERK pathways in phencyclidine-induced neurotoxicity in neonatal rats and the protection by lithium and BDNF
… neurotrophic factor (BDNF). In corticostriatal slice cultures, we found that lithium and BDNF, inhibited PCP-induced caspase-3 activation and DNA fragmentation in a concentration-dependent manner, respectively. Lithium and BDNF also prevented the inhibitory action of PCP on the …
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Investigating cestode modulation of host neuronal excitability and cell-type-specific gene expression in an in vitro mouse model of neurocysticercosis
… I exposed mouse hippocampal organotypic brain slice cultures (OBSCs) to lipopolysaccharide (LPS) and a Taenia crassiceps homogenate and used singlenucleus RNA sequencing and whole-cell patch-clamp electrophysiology to investigate potential links between inflammation and network excitability at …
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Investigating Synapse Function In Organotypic Hippocampal Slice Culture Models of Neurodegeneration
Organotypic hippocampal slice cultures (OHSCs) are a useful in vitro model for researching synapse dysfunction; they provide easy access to the tissue for biomarker analysis, whilst retaining much of the neuronal architecture and endogenous cell types, representing a reasonable compromise between …
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