Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 332 for “"site-directed mutagenesis"”.
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Site-Directed Mutagenesis in Francisella Tularensis by Allelic
… tools have been developed that have enabled mutagenesis of F. tularensis type A and type B stains. However, site-specific mutations remain difficult to execute or these methods generate random mutations. In this study a novel method was developed to create site-directed mutations in a …
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Site-directed mutagenesis of the ncd microtubule motor protein
… mitosis in <i>D. melanogaster</i>. PCR-mediated site-directed mutagenesis was utilized to introduce specific mutations into pET/MC6, a construct containing the motor domain of ncd. Six mutations were generated, two at glutamic acid residue 656, two at proline residue 649, one at arginine residue …
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Characterization and site-directed mutagenesis of NifU from Azotobacter vinelandii
… Following the initial characterization, site-directed mutagenesis of conserved cysteinyl residues was performed in order to gain further insight into the structure/function relationship of NifU. Both the Fe protein and the MoFe protein of nitrogenase require processing by additional nif …
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Exploring Protein -Rna Interactions With Site-Directed Mutagenesis and Phage Display
… is required. We have applied phage display and site-directed mutagenesis to dissect principles of RRM-controlled RNA recognition. The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond individual contacts …
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Exploring Protein-Rna Interactions With Site-Directed Mutagenesis and Phage Display
… is required. We have applied phage display and site-directed mutagenesis to dissect principles of RRM-controlled RNA recognition. The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond individual contacts …
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Exploring protein-RNA interactions with site-directed mutagenesis and phage display
… is required. We have applied phage display and site-directed mutagenesis to dissect principles of RRM-controlled RNA recognition. The model systems we are investigating are U1A and CUG-BP1. In this dissertation, the molecular basis of the binding affinity of U1A-RNA beyond individual contacts …
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Site-directed mutagenesis of the nitrogenase MoFe protein from Azotobacter vinelandii
… ligands) within the α- subunit were targeted for site-directed mutagencsis studies. In order to define a range of acceptable substitutions, 35 specific site-mutants have been constructed, each with a different amino acid replacement at one of the four targeted positions. Previous studies indicated …
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Site-directed mutagenesis of the ATP-binding site in spinach rubisco activase
Spinach rubisco activase has two isoforms derived from alternate splicing with the larger polypeptide containing an additional 4 kD C-terminal domain. Both isoforms, with molecular masses of 41 kD and 45 kD, were synthesized and purified from the transformed E. coli containing the cDNAs. A …
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Studies and applications of protein prenylation using isoprenoid analogues and site-directed mutagenesis
The site-specific enzymatic modification of proteins offers a powerful tool both for the development of new research techniques and for the development of more effective therapeutics. Protein farnesyltransferase (PFTase) is a promising tool in the field of site-specific protein modification due to …
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Gene cloning, adjustable expression and site-directed mutagenesis of Escherichia coli adenylate kinase
… effect on the adenine nucleotide concentrations. Site-directed mutagenesis was used to investigate the location of the ATP and AMP binding sites on adenylate kinase and the mechanism of substrate inhibition. Two amino acids, phe86 and tyr133, which were found to be in close contact with the …
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A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation
… applicable and efficient method to carry out site-directed genetic manipulations of large plasmids has been identified. The ability to easily reintroduce the recombinant DNA molecules back into the original host organism was maintained.
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Investigating the Pyrr-Pyr mRNA Interaction Through Site-Directed Mutagenesis of the PyrR Protein
… a model for how PyrR binds pyr mRNA. Through site-directed mutagenesis studies six PyrR amino acid residues were identified as being directly involved in RNA binding and one additional amino acid residue was identified as possibly being involved in RNA binding. As a result of in vivo analysis …
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Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
… catalyzes the elongation of 16:1Δ9 to 18:1Δ11. Site-directed mutagenesis was used to change residues highly conserved among the ELO family to examine their potential role in the condensation reaction. Mutant EloAs were expressed in yeast and fatty acid methyl esters prepared from total cellular …
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Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii
Nitrogenase-catalyzed nitrogen fixation is a biochemically and genetically complex process requiring the participation of a number of different nif (nitrogen fixation) gene products. The nifF (electron transport), nifA (nif gene regulation) and nifE (FeMo-cofactor biosynthesis) genes from …
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Site-Directed Mutagenesis of Southern Bean Mosaic Virus (Cowpea Strain): in Search of a Viral Protease
… to be a catalytic residue of a serine protease. Site-directed mutagenesis was used to create a mutant with a glycine at this position, and coupled in vitro transcription/translation was used to prepare 3H labeled translation products. SDS-PAGE and fluorography were then used to assay for the …
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Exploring The Ligand Binding Site On The Dopamine Transporter By Photoaffinity Labeling And Site-Directed Mutagenesis
… its transport activity, but their binding site within the protein and the mechanism by which they block DA transport remain unclear. Two distinct ligand interaction sites at transmembrane domains (TMs) 1-2 and 4-6 have been identified using irreversible uptake-blockers. The current studies …
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Exploring the Quinol Oxidation Mechanism at the Qo-Site of Bc1 Complex Through Site-Directed Mutagenesis
The backbone amide groups of FL132 and FC134 give additional electron withdrawing force to the Rieske-type [2Fe-2S] cluster and the residues in the 132-134 positions play roles in anchoring the ISP to the surface of the Qo-pocket. FG133 has unique ϕ/psi angles that restrict the positions of …
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Ligand discrimination and inhibition of heme iron autooxidation by myoglobin: Site-directed mutagenesis of a synthetic gene
… atmospheric dioxygen (O$\sb2$). Without protein-directed discrimination between these ligand molecules, the basal production of CO from various catabolic processes would inhibit the reversible transport and storage of oxygen that is necessary for life. Until recently, the exact function of active …
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