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Showing 1 to 15 of 15 for “"site specific mutagenesis"”.
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The anti-fluorescein antibody active site: A single-chain site-specific mutagenesis study
Restriction data tranferred 2014-07-01T11:21:50-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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The Evolution of Enzymatic Activity in the Crotonase Superfamily: The Mechanism of the Reaction Catalyzed by 2-Ketocyclohexanecarboxyl-Coa Hydrolase
… the catalytic roles of residues in the active site. It was concluded that, unlike other stereochemically characterized crotonase homologs, this reaction must proceed via a Z-enolate. Based on the information derived from stereochemical studies and site-specific mutagenesis, a mechanism of the …
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Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery
… levels by transient transfection in 293 cells. Site-specific mutagenesis was used to insert all four construct sequences into the IGF-1 HisMax plasmid, and to insert a secretory sequence into all four construct plasmids. A stable cell line selection in 293 and CHO cells using Zeocin was …
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An Analysis of Bacteriophage Lambda Site-Specific Recombination Using IHF Binding Site Mutants
… of bacteriophage lambda. Mutations of the three sites to which this protein binds within the lambda attachment site were constructed using oligonucleotide-directed site-specific mutagenesis and were ananlysed for their effects on site-specific recombination. One group of mutations had alterations …
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A Mutational Analysis of Bacteriophage Lambda Site-Specific Recombination (In Vitro, Dna-Dna Interaction, Protein, Oligonucleotide-Directed)
Mutations within the lambda attachment (att) site were constructed by oligonucleotide-directed site-specific mutagenesis and analysed for their effects on site-specific recombination. The phenotype of mutations within the 15 base "core" region of sequence homology demonstrated that homology …
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Identification and functional characterization of microRNA regulatory elements in Brassicaceae
… hormones, while some others are tissue or cell specific. The understanding of how these microRNAs are regulated at the transcriptional level is just initiated. With the aim to understand the regulatory mechanism of plant microRNA in evolutionary tem, and identify the most relevant cisregulatory …
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DNA sequences required for efficient transcription of the estrogen regulated Xenopus laevis vitellogenin promoter
… essential for estrogen-regulated and liver-specific vitellogenin gene expression, we employed site-specific mutagenesis to create a variety of deletions, mutations, and insertions in the 5$\sp\prime$-flanking region of the vitellogenin B1 promoter. The effects of these mutations on promoter …
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Cloning and characterization of the 3' terminal regions of RNA from select strains of maize dwarf mosaic virus and sugarcane mosaic virus
… SCMV viruses. Homology studies identified the site of proteolytic cleavage between the nuclear inclusion II proteins and the coat proteins of these viruses. Additionally, oligodeoxynucleotide directed site specific mutagenesis alteration of the MDMV-B cDNA clone allows excision of the …
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The translation initiation site of satellite tobacco necrosis virus RNA does not require a start codon
… to examine this theory, oligonucleotide directed site specific mutagenesis of pSTNV$\sb{1239}$ and pSP65-A4 plasmids containing full length cDNA clones of STNV RNA and AMV-4 RNA was used to generate single nucleotide substitutions in the translation initiation region of these mRNAs. Gel …
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Mielių baltymo Sup35 GNNQQNY sekos mutantinių variantų kūrimas ir jų sintezė Saccharomyces cerevisiae ląstelėse /
… by PCR. Sequencing results confirmed that site-specific mutagenesis by PCR was successful in introducing selected mutations into the SUP35-GFP gene and that these mutations were not lost during all steps of the study. The constructed expression vectors pRSCupSup35-GFP containing the …
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Streptococcal M protein and human C4BP
… lack residue identities. However, use of site-specific mutagenesis to substitute relatively conserved residues in the M22 protein indicated that the predicted coiled-coil structure of the HVR is crucial for ability to bind C4BP. Interestingly, change of single residues that do not affect …
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Investigating The Metal Binding Sites In Znta, A Zinc Transporting Atpase
… out of the cytoplasm. ZntA has two metal binding sites, one in the hydrophilic N-terminal domain and the other in the transmembrane region. The cysteine-rich N-terminal domain has ~110 amino acids and the conserved motifs -CCCDXXC- and -DCXXC-. The role of these motifs in metal-binding specificity …
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Role of the LPA2 Receptor in Protecting Against Apoptosis
… signaling networks. Using deletion and site-specific mutagenesis, we mapped out C311xxC314 motif of LPA<sub>2</sub>-CT that is required for interaction with LIM domain proteins thyroid hormone receptor interactive protein 6 (TRIP6) and the proapoptotic molecule Siva-1 <em>in vitro</em> …
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Mechanistic Studies of the Roles of the Transcriptional Activator ExsA and Anti-activator Protein ExsD in the Regulation of the Type Three Secretion System in Pseudomonas aeruginosa
… itself is highly complex and, together with the specific secreted factors, requires expression of more than 30 genes. Due to the high energy cost of its synthesis to the organism this system is highly regulated to finely time gene expression to coincide with host contact. ExsA, a member of the …
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Untersuchungen zur genetischen Regulation der CO<sub>2</sub>-Assimilation in <i>Ralstonia</i> spp.
Die autotrophe CO2-Assimilation erfolgt in Ralstonia eutropha H16 und Ralstonia metallidurans CH34 über den des Calvin-Benson-Bassham (CBB) Cyclus. Die Expression der beiden hoch homologen cbb-Operone cbbc und cbbp in R. eutropha H16 wird in erster Linie durch den LysR-Typ-Transkriptionsregulator …