Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"single-molecule microscopy"”.
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Seeing the invisible : single molecule microscopy in the shortwave infrared
… is known about their optical properties at the single nanoparticle level. In this thesis, we detail efforts to extend the power of single-molecule spectroscopy into the shortwave-infrared (SWIR), marrying confocal microscopy with superconducting nanowire single photon detectors that operate very …
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NUCLEAR ENVELOPE TRANSMEMBRANE PROTEIN DISTRIBUTION AND TRANSPORT STUDIED BY SINGLE-MOLECULE MICROSCOPY
… mediate the bidirectional trafficking of molecules between the nucleus and cytoplasm by forming a barrier. NE transmembrane proteins (NETs) embedded in either the outer nuclear membrane (ONM) or the inner nuclear membrane (INM) play crucial roles in both nuclear structure and functions, …
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Characterising the early aggregation events of human lysozyme using single-molecule microscopy
… fluorescently labelled protein, we have used single-molecule fluorescence microscopy techniques including two-colour coincidence detection (TCCD) and fluorescence resonance energy transfer (FRET) to determine the oligomer population distributions present during the aggregation of HuL. Our data …
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Molecular Size and Charge Effects on Nucleocytoplasmic Transport Studied By Single-Molecule Microscopy
… passive diffusion of small signal-independent molecules (< 20-40 kDa) and transport-receptor facilitated translocation of signal-dependant cargoes. The molecular size of a cargo molecule is one of the important parameters that determine the final transport mode for its transport through the …
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Single-molecule microscopy reveals details of heterochromatin reorganisation at the onset of mouse ES cell differentiation
… how chromatin folds – from the level of single nucleosomes all the way to compartments and chromosomal territories – it is still unclear what determines this structure and why it changes upon differentiation. Mouse embryonic stem cells provide an excellent system to address these …
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Image Deconvolution Techniques for Single Molecule Studies
… is the need to analyze information from single proteins as opposed to ensembles of many molecules. Consequently, the advent of high-resolution imaging in single molecule microscopy has enabled researchers to probe the underlying processes of gene regulatory networks and other biological …
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Convex lens induced confinement : a novel study of weak and slow DNA & protein interactions
The newly developed single molecule microscopy method of flow cell-convex lens induced confinement (FC-CLIC) allows for the previously unobtainable probing of slow and weak DNA and protein interactions. Such measurements are made possible by CLIC's simultaneously improved signal-to-background …
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Single molecule fluorescence microscopy of carbon nanotubes
Single molecule microscopy has been extensively used in the past decade to study individual biomolecules with excellent spatial and temporal resolution. Meanwhile, characterization of nanomaterials and their development towards a variety of biological applications has progressed rapidly. Despite …
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Single-molecule orientation estimation using a polarisation camera
… for measuring the orientation and position of single fluorescent molecules. The method - named POLCAM - is based on the use of a polarisation camera. Current methods for single-molecule orientation estimation require optical setups and algorithms that can be prohibitively slow and complex, …
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Single-molecule studies reveal mechanisms of human DNA double-strand break repair
… damages are double-strand breaks (DSBs), and a single unrepaired DSB can lead to cell death. In human cells, there exist two canonical pathways of DSB repair: Non-Homologous End Joining (NHEJ) and Homologous Recombination (HR). Two protein complexes that rapidly bind DNA ends coordinate these …
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Single-Molecule and Ensemble Measurements Inform on the Role of Protein Crowding at Interfaces
… interaction between different proteins remain. Single-molecule high-resolution imaging with photobleaching (SHRImP) was applied to measure dye-conjugated fibronectin’s unfolding in varying conditions of crowding with serum albumin on aminosilanized glass. Our results indicated that serum albumin …
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Advances in Microfluidics and DNA Nanotechnology for Biomolecular Analysis
The sensing and characterisation of biomolecules is crucial for medical diagnostics and an understanding of biological systems. Proteins play a leading role in biology, by performing the majority of biochemical and biophysical processes that maintain homeostasis. As such, they and their …
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Monte Carlo Methods in Practice and Efficiency Enhancements via Parallel Computation
… task of performing parameter inference in single-molecule microscopy, a category of models in which the arrival rate of observations is not uniformly distributed and measurement models have complex forms. These issues are exacerbated when molecules have trajectories described by stochastic …
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3D tracking of solute dynamics in heterogeneous polymer networks
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms
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Mammalian genome engineering: Fundamental investigation and method development
… and dCas9 proteins in live-mammalian cells using single molecule microscopy. The first part focuses on elucidating TALE search mechanism in vivo. For the first time, TALE genome-search has been visualized in real-time in live-cells and search kinetics were extracted. The results show that TALEs …
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Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction
… activation of SMM filaments by MLCK using single molecule fluorescence microscopy. Our lab has previously investigated the kinetics and the mechanics of MLCK interactions with monomeric SMM (Hong et al (2013) Biochemistry 52, 8489-8500) as well as with purified F-actin and skinned smooth …
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Making Molecular Movies: Using Single-Molecule Techniques to Unveil Hidden Features of Protein-Chromatin Interactions
… activities in real-time, owing to the advent of single-molecule fluorescence- and force-based techniques that have enabled dynamic and quantitative measurements of protein-chromatin interactions central to nuclear biology. This thesis describes my work, in collaboration with others, at harnessing …
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Revealing SMC5/6's Dynamic Behavior on Diverse DNA Topologies Through Single-Molecule Methods
… double-stranded DNA (dsDNA), stretches of single-stranded DNA (ssDNA), chromatinized DNA, forked/junction DNA, and recombination intermediates. In this thesis, our approach was to utilize correlative single-molecule fluorescence and force microscopy, which combined optical tweezers, …
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AN EXPLORATION OF DEFECT-BASED SINGLE PHOTON SOURCES: ZINC OXIDE AND HEXAGONAL BORON NITRIDE
… point defects in wide bandgap semiconductors are single photon sources with applications in quantum optics, precision sensing, and quantum information processing technologies. Although the nitrogen-vacancy center in diamond has historically garnered the most attention, efforts to discover novel …