Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"single-molecule methods"”.
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Single molecule methods for clinical biomarker quantification
… improved bioanalysis platforms, state of the art methods remain dependent on incredibly complex and time consuming techniques which exhibit insufficient sensitivity and minimal capacity for multiplexing. This thesis aims to address these limitations through the design of improved molecular …
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Revealing SMC5/6's Dynamic Behavior on Diverse DNA Topologies Through Single-Molecule Methods
… double-stranded DNA (dsDNA), stretches of single-stranded DNA (ssDNA), chromatinized DNA, forked/junction DNA, and recombination intermediates. In this thesis, our approach was to utilize correlative single-molecule fluorescence and force microscopy, which combined optical tweezers, …
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Single-molecule methods for an improved understanding of biophysical interactions: from fundamental biology to applied nanotechnology
The advent of single-molecule methods has greatly extended the scale at which we are able to probe natural systems. The information that can be gained by studying biological systems on a single-molecule scale, in the absence of ensemble averaging, provides an unprecedented amount of detail about …
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Methods of single-molecule energy landscape reconstruction with optical traps
… facilitate measurement of force and position as single molecules of DNA, RNA, or protein are unfolded and refolded. The effective energy landscape of a biomolecule can be reconstructed from the force and position data, providing insight into its structure and regulatory functions. We have …
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Regulation mechanisms in molecular motors: engineering bioinspired transportation at micron-scale
… instrumentational, computational, and DNA-based single-molecule methods. These diverse approaches converge to unveil unprecedented insights into the coordination mechanisms of molecular motors and the regulation of intracellular cargo transport. The research explores the impact of various …
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Deconstructing the room-temperature emission spectra of nanocrystals using Photon-Correlation Fourier Spectroscopy
… result of the homogeneous "natural" spectra of single emitters subjected to interparticle inhomogeneities and perturbations from the environment. For semiconductor nanocrystals (NCs), efforts to tune ensemble linewidths for optical applications have focused primarily on eliminating sample …
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Single-molecule profiling of tau aggregates in tauopathies: From test tube to patient
In recent decades, single-molecule methods have transformed the physical sciences, offering unprecedented insights into the complexity of biological systems at molecular resolution. In particular, fluorescence-based methods now enable the detection and imaging of individual biomolecules and their …
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Single-Molecule Investigation of Chromatin-Associated Factors in Genome Organization and Epigenetic Maintenance
… to study chromatin-associated factors at the single-molecule level (Chapter 2). This methodology allows us to extract key mechanistic details often obscured by standard bulk methodologies. Using this platform, we posed the question of how epigenetic factor, Polycomb repressive complex 2 (PRC2) …
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Agonist-Induced Conformational Changes In The Nmda Receptor
… these receptors bind to glycine and glutamate molecules and undergo a series of conformational changes that results in the opening of a cation-permeable pore in the neuronal plasma membrane. Various aspects of NMDA receptor function are not fully understood, including the phenomenon of negative …
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Single Molecule Fluorescence Studies of Prions and Prion-like Proteins
… and Parkinson’s disease. In this thesis, I use single-aggregate imaging to study fibril fragmentation and elongation of individual murine PrP aggregates from seeded aggregation in vitro. From fluorescence imaging of individual PrP aggregates on the coverslip surface, elongation and fragmentation …