Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"single-molecule localization microscopy"”.
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Convex Relaxations for Particle-Gradient Flow with Applications in Super-Resolution Single-Molecule Localization Microscopy
<p>Single-molecule localization microscopy (SMLM) techniques have become advanced bioanalytical tools by quantifying the positions and orientations of molecules in space and time at the nanoscale. With the noisy and heterogeneous nature of SMLM datasets in mind, we discuss leveraging …
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Super-Resolved Microscopy as a Tool to Unveil Intracellular Structure in Biological Systems
… have employed a combination of super-resolution microscopy techniques to explore the spatial redistribution of the heat-stable nucleoid structuring (H-NS) protein within E. coli in response to high osmolarity. H-NS is an abundant nucleoid association protein in gram-negative bacteria and plays a …
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Spatio-temporal properties of membrane-localized actin nucleating complexes
… by interactions with actin and exchange of molecules with solution. Single-molecule localization microscopy resolved the nanoscale organization of Toca-1, showing its arrangement into flat plaque-like and narrowly elevated tubular substructures. Plaque-like structures showed similarities to …
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Super-Resolution Fluorescence Microscopy using Transparent Waveguide Chips
Super-resolution fluorescence microscopy (optical nanoscopy) allows us to surpass the optical diffraction limit and observe biological phenomena at the nanoscale using visible light. Different modalities come with their own trade-offs, such as imaging speed vs. achieved resolution or live cell …
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Synthetic ground truth of biological shapes — Simulating variable Nuclear Pore Complexes for Microscopy
… Complex (NPC) is the only passageway for macromolecules between the nucleus and cytoplasm and an important reference standard in microscopy: it is intrinsic to cells, has a high copy number, it is massive and stereotypically arranged. The average architecture of NPC proteins has been resolved …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
Single-molecule super-resolution fluorescence microscopy is a powerful method for imaging detailed biological structures at the nanoscale. However, imaging capabilities in thick samples such as mammalian cells remain limited due to increased fluorescence background which degrades the achievable …
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Structural Organization and Chemical Activity Revealed by New Developments in Single-Molecule Fluorescence and Orientation Imaging
<p>Single-molecule (SM) fluorescence and its localization are important and versatile tools for understanding and quantifying dynamical nanoscale behavior of nanoparticles and biological systems. By actively controlling the concentration of fluorescent molecules and precisely localizing individual …