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Showing 1 to 12 of 12 for “"single-molecule Förster resonance energy transfer (smFRET)"”.

  1. Effect of oxidative stress on human telomeric DNA structural dynamics and accessibility

    Kim, GraceUniversity of Illinois at Urbana-Champaign2015

    The ends of human telomeric DNA consist of single-stranded overhangs of tandem TTAGGG repeats (ssTEL) that can form into a G-quadruplex (GQ). This guanine-rich sequence is prone to 8-oxoguanine (8OG) damage, which is one of the most common oxidative DNA lesions that can compromise telomere …

    uiuc Repository record

  2. Understanding the activation-deactivation mechanism of glutamate receptor proteins at the single molecule level

    Chatterjee, SudeshnaRice University2019

    … the ion channels are controlled by a number of molecules (ligands) interacting with specific binding sites. Hence, these binding sites can serve as potential drug targets for controlling the aberrant activation of the ion channel that leads to a number of neurological disorders. In this …

    rice Repository record

  3. Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time

    Vrtis, Kyle2013

    … In 2009, Christian, et al. developed a single molecule Förster resonance energy transfer (smFRET) approach to monitor the polymerase position on the DNA with single base pair resolution. </p> <p> We have worked to optimize nearly every aspect of that approach and to apply the technique …

    wayne-thes Repository record

  4. Fret-Based Investigations of The Structure-Function Relationships In The Nmda Receptor

    Dolino, Drew M, <p>0000-0002-2690-7140</p>2017

    … function. The work in this dissertation uses single molecule Förster Resonance Energy Transfer (smFRET) as a means to address that gap. We have examined the question of partial agonism of the NMDA receptor, noting a mechanism of a dynamically graded cleft closure. We have pushed the bounds of …

    uthsc Repository record

  5. QUANTIFYING OLIGOMERIC STATES OF PROTEINS IN-CELL VIA SINGLE-MOLECULE SUPER-RESOLUTION MICROSCOPY

    Xie, XihongUniversity of Houston2022

    … describes our efforts in developing in-cell single-molecule imaging assays to quantify oligomeric states of membrane and cytosolic proteins. Quantifying oligomeric states of highly-expressed membrane proteins were achieved using protein localizations obtained from super-resolution microscopy. …

    houston Repository record

  6. Examining Post-Translational Modifications In Alpha-Synuclein Through Semi-Synthesis And Single Molecule Spectroscopy

    Pan, Buyan2021

    … effects for arginylation at more than one site. Single molecule Förster resonance energy transfer (smFRET) measurements reveal changes in monomer conformation that underlie a possible mechanism for the differential aggregation profile of phosphorylated αS. In addition to its disease-relevant …

    penn Repository record

  7. Single Molecule Förster Resonance Energy Transfer and Super Resolution Force Spectroscopy Study of Elongation Factor G Evolution, Conformation, and Function

    Steele, Jacob Hunter 1993-University of Houston2025

    … tRNA translocation via the hydrolysis of one GTP molecule. The mechanism of this reaction remains largely unknown. Cryo-EM studies indicated that the switch I region of EF-G undergoes major conformational changes following GTP hydrolysis, which may drive tRNA translocation. Multiple sequence …

    houston Repository record

  8. Single-Stranded Dna Scanning And Deamination With Single-Molecule Resolution

    Senavirathne, Padhuk Gamarala2013

    <p>SINGLE-STRANDED DNA SCANNING AND DEAMINATION WITH SINGLE-MOLECULE RESOLUTION</p> <p>by</p> <p>GAYAN SENAVIRATHNE</p> <p>December 2013</p> <p>Advisor: Dr. David Rueda & Dr.Ashok Bhagwat</p> <p>Major: Chemistry (Analytical) </p> <p>Degree: Doctor of Philosophy</p> <p>APOBEC3G (Apo3G) and …

    wayne-thes Repository record

  9. The role of Orc6 in ORC binding-site switching during helicase loading

    Driscoll, DavidMassachusetts Institute of Technology2024

    … formation of the Mcm2-7 double hexamer. Previous single-molecule studies show that one ORC can recruit and load both Mcm2-7 helicases at origins. This one-ORC helicase-loading model requires a dramatic change in ORC location to transition between its primary and secondary binding site. …

    mit Repository record

  10. Single molecule fluorescence studies of replicative helicases

    Syed, SalmanUniversity of Illinois at Urbana-Champaign2014

    … are motor enzymes that convert the chemical energy of nucleotide triphosphate hydrolysis into mechanical energy for translocation on single stranded (ss) DNA and unwinding of double stranded (ds) DNA. All of the 12 known replicative helicases are hexameric helicases that are ring-shaped in …

    uiuc Repository record

  11. Single-molecule studies on the accessibility of chromatin : post-translational modifications and nucleosome remodeler CHD1

    Quack, SalinaUniversität Ulm

    ulm-diss Repository record

  12. Evolution and assembly of the ribosome

    Chen, KeUniversity of Illinois at Urbana-Champaign2014

    … ribosome consists of three ribosomal RNA (rRNA) molecules and approximately fifty ribosomal proteins (r-proteins), which are arranged into two subunits of unequal size and distinct function. The large subunit promotes formation of the peptide bond, and the small subunit enforces the recognition …

    uiuc Repository record