Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"single-domain antibody"”.
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Over-expression in Escherichia coli of the ToxA51 llama single domain antibody targeting Clostridium difficile enterotoxin A
Single domain antibodies (sdAb) have emerged as a family of novel antibodies that have vast applications. Of particular relevance, ToxA5.l, a sdAb capable of binding to, and therefore neutralizing, Clostridium difficile toxin A (TcdA) has been developed. The antibiotic resistant version of C. …
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Rational Design and Application of Antibodies for Amylin and Amyloid Beta
… and affinity for targets. However, traditional antibody discovery methods often fail to produce antibodies with desirable properties such as epitope selectivity, conformational stability, and solubility. Computational antibody design methods combining epitope targeting, solubility calculation …
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Developing VHH-based tools to study Ebolavirus infection
Variable domains of camelid-derived heavy chain-only antibodies, or VHHs, have emerged as a unique antigen binding moiety that holds promise in its versatility and utilization as a tool to study biological questions. This thesis focuses on two aspects on developing tools to study infectious …
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T-cell-directed bioorthogonal catalysis for localised prodrug activation in cancer therapy
… are used as an inducible antigen to recruit antibody-catalysts that facilitate drug activation in tumour proximity. To demonstrate this concept, we integrated a bioorthogonal catalyst with a single-domain antibody and demonstrated catalytic properties. A T-cell derived cell line was …
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Transcriptomics-Guided Biodiscovery of Peroxidases from Macroalgae
… expressed in Escherichia coli as a fusion with a single-domain antibody (VHH). The fusion protein was successfully applied as a reporter enzyme in an ELISA, demonstrating both target binding and a detectable signal. Overall, this work establishes a transcriptome-based approach for enzyme discovery …
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Engineering VHH-based chimeric antigen receptor (CAR) T cell therapy for solid tumor treatment
… markers in their microenvironment. The use of single domain antibody (VHH)-based CAR T cells that recognize these markers circumvents the need for tumor-specific targets. Chapter 3 will describe methods to overcome the immunosuppressive microenvironment of solid tumors. Here, we have developed …
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Developing a novel hepatitis B core – based antigen presentation system
… antigens either via chemical coupling or through antibody-antigen interactions. Of particular significance was the successful display of single domain antibody fragments of camelid origin (nanobodies, or VHH). The resulting “tandibody” particles, as they are named here, can bind to their cognate …
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Chemical Mutagenesis for the Modulation and Study of Protein Function and Dysfunction within Alzheimer’s Disease
… a structure-activity relationship study on a single-domain antibody (SdAb) beyond the 20 canonical AAs. The starting SdAb was designed to target the Amyloid-b peptide (Ab) and has a nascent ability to inhibit its aggregation, a key pathological process in AD. Utilizing the synthetically …
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Cross-reactivity among alphaviruses provides insight into viral emergence and novel defense strategies
… characterized the potency of a camelid-derived single-domain antibody (sdAb) that neutralizes a breadth of alphaviruses, including CHIKV and MAYV. With these data, we have designed and generated transgenic Aedes aegypti mosquitoes that express two anti-CHIKV sdAbs to target infection, …
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Using data to characterise the relationship between nanobody sequence, structure and function
Nanobodies (Nbs) are a class of single-domain antibody derived from the immune systems of camelid species. They achieve binding affinities and specificities to target antigens comparable to those of classical antibodies (Abs), despite being ten times smaller (~15 kDa) and having only three variable …
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Studies on the efficient intracellular delivery vehicle using variable region domain of a cell-penetrating anti-DNA monoclonal antibody
The cell-penetrating antibodies and antibody fragments can be utilized as a tool for the medical diagnosis and treatment. In this study, we have produced the protein of recombinant VH domain of a monoclonal anti-DNA antibody 2C10, IgG of which has been previously shown to have cell-penetrating …
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Rational Design of Antibodies against Tau and Alpha-Synuclein
… In earlier studies, an oligomer-specific antibody against Abeta was created to detect and quantify oligomers by applying a rational antibody design approach known as the cascade method. Here, we used this method to design a library of antibodies (DesAbs) systematically covering the length …
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Development of VHH- and antibody- based imaging and diagnostic tools
… a site for labeling in a controlled manner. Single-domain antibodies (VHHs) from camelids are amongst the smallest (15 KDa) proteins that can recognize a diverse set of targets with excellent specificity. Chemoenzymatic labeling of molecules using sortase allows site-specific attachment of a …
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Functional and Molecular Imaging Using Nanoparticle Contrast Agents for Dual-Energy Computed Tomography
… in which two x-ray energies are used for a single scan, can provide valuable information about tissue material composition. This information can potentially be used for molecular imaging if it is coupled with appropriately-designed contrast agents. </p><p>This work aims to extend the use of …