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Showing 1 to 20 of 28 for “"single-chain variable fragment(scFv)"”.

  1. Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting

    노, 주호2014

    3D8 single chain variable fragment (scFv) derived from a MRL-lpr/lpr mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against …

    ajou Repository record

  2. Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv

    김, 혜진2014

    … cells. An anti-nucleic acid antibody, murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv internalization remains unknown. Here, we studied …

    ajou Repository record

  3. Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments

    이, 정민2016

    3D8 single chain variable fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and …

    ajou Repository record

  4. Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication

    정, 의정2011

    3D8 single chain variable fragment (scFv) is a recombinant anti-DNA antibody from an autoimmune-prone MRL-lpr/lpr mouse with lupus diseases. 3D8 scFv can hydrolyze ss-DNA, ds-DNA, ss-RNA, and ds-RNA without sequence specificity. On the base of 3D8 scFv property, we carried out quantitative and …

    ajou Repository record

  5. Characterization of anti-DNA antibody with Tat peptide sequence in CDR

    정, 종근2011

    … 항-DNA 항체 구조의 생화학적 특성 항-DNA 항체인 3D8 단일 가닥 가변부위 (single chain variable fragment: scFv)는 핵산결합능 및 가수분해활성이 있으며, 3D8 VL 단독부위만으로도 핵산 결합능 및 가수분해능을 발휘한다. 또한 3D8 scFv는 세포막 침투능이 있어서, 세포막을 투과한 후, 세포질에 위치하게 되고, 결국은 세포사멸을 유도한다고 알려져 있다. 본 연구에서는 3D8 scFv의 핵산가수분해 활성은 그대로 유지하면서, 세포막 투과 후 핵으로 이동할 수 있는 3D8 scFv …

    ajou Repository record

  6. Synergic Effect on Cell Death by Co-treatment with TRAIL and Human Anti-DR5 Antibody

    김, 태인2011

    … cells. In previous study we isolated a human single-chain variable fragment (scFv) antibody (HW1) that recognizes a different epitope on DR5 form that of TRAIL and induced autophagic cell death. when treated to various cancer cell lines. To study the interaction between TRAIL-induced apoptosis …

    ajou Repository record

  7. Antibody phage-displayed libraries derived from chicken immunoglobulin genes : a source of highly specific diagnostic antibodies

    University of Pretoria2008

    … and aldolase (ALDO), recombinant trypanosome variable surface glycoprotein (VSG) and malignant catarrhal fever virus (MCFV) each chicken produced egg yolk antibodies (IgY) against four of the five antigens. Using phage display technology, two single-chain variable fragment (scFv) antibody …

    pretoria Repository record

  8. Novel anitbody-based assays for disease and food contaminant detection

    Stapleton, SharonDublin City University2007

    … Irish herds. A previously isolated Am1-specific single chain variable fragment (scFv) was converted to a chimeric Fab format by the addition of human constant regions, using PCR assembly methods. A mutant Fab fragment was also isolated and demonstrated improved assay performance when compared to …

    dcu Repository record

  9. Antiviral activity of 3D8 single chain Fv against RNA virus infection

    전, 혜령2012

    … as Abzymes. Previously it was reported that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by electrostatic interaction with cellular membrane and it localizes in cytosol resulting in cell death by cellular rRNA degradation. These result mean that 3D8 scFv would also have …

    ajou Repository record

  10. Development of monoclonal antibodies targeting T cell coinhibitory proteins TIM-3 and TIM-4

    University of Malta2024

    … been developed yet. In this study, monoclonal single chain variable fragment (scFv) antibodies against TIM-3-Fc and TIM-4 were identified using phage display biopanning which isolated antibodies from large immunoglobulin gene repertoires displayed on the surface of bacteriophages. Thirty …

    malta Repository record

  11. Plant-expressed diagnostic proteins and their use for the identification and differentiation of infected and vaccinated animals with foot-and-mouth disease virus

    De Beer, ScottDepartment of Molecular and Cell Biology2017

    … reagent to be expressed: this was the CRAb-FM27 single chain variable fragment (scFv), which binds a 3B epitope on the 3ABC polyprotein and has previously shown to be effective as a competing antibody in a C-ELISA. The 3B antigen and the scFv were successfully expressed and purified from N. …

    cape-town Repository record

  12. Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing

    Larman, Harry BenjaminMassachusetts Institute of Technology2012

    … determining regions (CDRs; L3 from the light chain, H2 and H3 from the heavy chain), and were combinatorially cloned into a synthetic single chain variable fragment (scFv) framework for molecular display. Our novel CDR sequence design utilized a hidden Markov model (HMM) that was trained on …

    mit Repository record

  13. Monoclonal and phage-displayed antibodies as lipopolysaccharide serotyping reagents and their use in typing colony variants of Vibrio vulnificus

    Schulz, Randall M.Biological Sciences2022

    … by having DNA coding for its light and heavy chains cloned into a phage-display system. The published primers, designed to be used with the pComb3X display system, produced primer dimers and were unsuccessful in generating the single chain variable fragment (scFv) to be cloned into the phage …

    lsu-thes Repository record

  14. Development of a Dusky kob scFv gene phage display library for the discovery of antibodies to Brome mosaic virus - a proxy for a novel, emerging fish pathogen

    Naylor, Kyle AndrewDepartment of Molecular and Cell Biology2021

    … by a diverse repertoire of genes coding for the single chain variable fragment (scFv) of immunoglobulin M can be isolated and screened for affinity to a specific infectious agent or parasite. In this study, a phage display library displaying scFvs derived from combination pairings of Dusky kob …

    cape-town Repository record

  15. Engineering scFv Antibodies for Point-of-Care Diagnostics

    Manohar, MadhuriUniversity of Cambridge2024

    … measure serum CysC. By engineering CysC-specific single chain variable fragment (scFv) antibodies with a cellulose binding tag (CipA) to tether it to paper, and a fluorescent protein (mCherry) to provide a detectable signal, the aim is to develop a paper-based sandwich immunoassay that can be used …

    cambridge Repository record

  16. Development and characterization of recombinant human autoantibody using cDNA made from peripheral blood mononuclear cells of patients with bronchial asthma

    조, 수미2015

    … 3 2. Total RNA 추출 4 3. mRNA 분리 4 C. Polymerase chain reaction (PCR)을 이용한 cytokeratin 18 (CK-18) 단백질에 대한 인간 single-chain variable fragment (scFv) 항체 라이브러리의 구축 5 1. cDNA 합성과 중합효소 연쇄반응 (PCR) 5 2. scFv의 heavy chain과 light chain 합성 5 3. Overlapping PCR을 통한 인간재조합 scFv의 제작 5 4. Ligation 6 5. Library …

    ajou Repository record

  17. Studies on voltage-gated sodium channel β3 subunit structure and cancer-related functions using single-chain variable fragment antibodies and bioinformatics

    Liu, HengruiUniversity of Cambridge2024

    … tissues. This thesis describes the use of single-chain variable fragment (scFv) antibodies to study the binding site of the β3 subunit on the pain-sensing channel, Nav1.7, and investigate the role of the VGSC β3 subunit in cancer guided by bioinformatic analysis. *In silico* docking was …

    cambridge Repository record

  18. Precision Cytokine Therapy: Engineering and Delivery of CD8- and CAR-Tropic Interleukin-2 Variants

    Ghodsinia, ArmanUniversity of Cambridge2025

    … for CD8+ T cells via fusion to a CD8-targeting single-chain variable fragment (scFv). This construct, termed scFv-IL2, displayed over one million-fold greater selectivity for CD8+ T cells compared to wild type IL2. AAV mediated expression of scFv-IL2 in the brain increased CD8+ T cell numbers by …

    cambridge Repository record

  19. Antibody engineering to evaluate binding, internalisation, and intracellular routing of tumour-targeting fusion proteins

    Karaan, MaryamDepartment of Health and Rehabilitation Sciences2023

    … fusion protein format for ADCs using a single chain variable fragment (scFv) specific to chondroitin sulphate proteoglycan 4 (CSPG4), a tumour-associated antigen often overexpressed in TNBC. The scFv antibody derivative was genetically fused to a SNAP-tag, enabling stable and …

    cape-town Repository record

  20. Development of potential immunodiagnostic & therapeutic techniques using SNAP-fusion proteins as tools for the validation of Triple-negative Breast Cancer

    Magugu, Freddy-Junior SiybaulelaDepartment of Integrative Biomedical Sciences (IBMS)2020

    … strategies has led to applications of single chain variable fragment (scFv) & single domain/nanobody (VHH) antibody formats for diagnostic and therapeutic purposes. In this work, these recombinant antibody fragments have been combined with SNAP-tag, a modified version of the human DNA …

    cape-town Repository record

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