Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 158 for “"single-chain"”.
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Single-chain nanoparticle based catalysts
… The three-dimensional structure of the peptide chains creates a local environment that supramolecularly binds substrates to facilitate the catalysis. In this dissertation, synthetic polymers are used to serve a similar role, binding substrates and creating an optimal environment for performing …
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Characterization of a single-chain anti-T cell receptor antibody and a single-chain T cell receptor
… cell. The TCR is made up of two disulfide-linked chains (called $\alpha$ and $\beta$) each containing a variable and a constant domain. The tertiary structure and binding properties (e.g. affinity and kinetics) of the TCR have not been solved. Additionally, the T cell has been implicated in many …
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Engineering human single-chain T cell receptors
… development of engineering strategies for human single-chain T cell receptor variable fragments (scTv), with the goal of understanding the properties that allow scTv to be expressed and deployed in a therapeutic mode. Stable scTv receptors can be used to generate high-affinity TCRs specific for …
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Single-chain polymeric systems: design, synthesis, and applications
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-05-01
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Single chain MHC trimer-based DNA vaccines for pathogen protection
… proteins on the cell surface, termed single chain trimers: SCTs). SCTs are composed of an immunodominant peptide, β2m, and MHC I heavy chain covalently linked by 15-20 amino acid flexible linkers. Because SCTs are expressed as a single polypeptide chain, they do not require …
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Engineering and characterization of human single-chain T cell receptors
… affinity TCRs using yeast display of TCRs in a single-chain format (scTCR) that consists of the variable regions of the α and β chains connected by a flexible linker (Vα-linker-Vβ or Vβ-linker-Vα). The focus of this dissertation is the engineering of human high affinity scTCRs for the targeting …
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Design of Single-Chain Polymer Nanoparticles to Mimic Globular Proteins
… synthetic alternatives with higher stability. Single-chain polymer nanoparticles (SCNPs) based on random heteropolymers (RHPs) have emerged as a promising platform as both protein stabilizers and mimetics. However, theoretical understanding of the origins underlying their functional versatility …
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Antiviral activity of 3D8 single chain Fv against RNA virus infection
… as Abzymes. Previously it was reported that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by electrostatic interaction with cellular membrane and it localizes in cytosol resulting in cell death by cellular rRNA degradation. These result mean that 3D8 scFv would also have …
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Biophysical characterization of high affinity engineered single chain Fv antibody fragments
… antibodies for very high affinity binding. Two single chain antigen-binding fragment (scFv) antibody systems that were engineered to obtain higher affinity interactions through directed evolution were probed using biophysical techniques to illuminate affinity modulation in proteins. First, …
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Molecular Engineering of a Single-Chain FV Antibody Fragment to Femtomolar Affinity
Mutants of anti-fluorescein scFv antibody 4-4-20 improved more than 1000-fold with respect to ligand dissociation kinetics and equilibrium binding affinity have been isolated from mutagenized libraries displayed on yeast and screened by flow cytometry. Analysis of the nature of mutations conferring …
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Engineering and Characterization of a Single Chain Antibody Specific for Murine CD28
Finally, a novel method of measuring the thermal stability of engineered antibody fragments using yeast surface display and flow cytometry was produced. This method allowed for the rapid determination of relative temperature stability without the need to produce large quantities of protein. This …
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Expression of single chain antibody-encoding genes in fission yeast and tobacco
… was to explore the expression of genes encoding single chain antibodies (SCA) in eukaryotic cells. During the course of this research, a new PCR-based strategy was developed that significantly reduced the time required for SCA gene synthesis. Although initially developed for the synthesis of SCA …
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Enzyme-like catalysis by single chain nanoparticles that use transition metal cofactors
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-12-01
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The anti-fluorescein antibody active site: A single-chain site-specific mutagenesis study
Restriction data tranferred 2014-07-01T11:21:50-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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Recombinant antibodies for the study of livestock infection: from basic genetics to single-chain Fvs
… of the third antigen-binding region of the heavy chain) appear unique. The first area for investigation in this project was to characterise the bovine JH locus and to understand why Ig rearrangement apparently favours a single JH segment. PCR was used to recover JH sequence from genomic DNA, …
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Herstellung und Charakterisierung rekombinanter Immunotoxine aus anti-PSMA single-chain Antikörperfragmenten zur Therapie des Prostatakarzinoms
… dem monoklonalen Antikörper 3A12 wurde zudem ein single-chain-Antikörperfragment (scFv) rekombinant hergestellt. Das scFv, A5 genannt, konnte durch Anwendung der Phage-Display-Technik und durch direkte Selektionierung auf antigen-tragenden Zellen gewonnen werden. A5 wies die gleichen …
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Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
3D8 single chain variable fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and …
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Development of a novel antibody molecule for redirected T cell immunity to tumor cells: The bispecific single chain antibody
Cancer is the one of the leading causes of death in the United States, and will affect one in three people in their lifetime. In addition to existing treatments, immunotherapy is emerging as a potential fourth tier of cancer therapy. The existence of tumor specific proteins and the production of …
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Studies on voltage-gated sodium channel β3 subunit structure and cancer-related functions using single-chain variable fragment antibodies and bioinformatics
… tissues. This thesis describes the use of single-chain variable fragment (scFv) antibodies to study the binding site of the β3 subunit on the pain-sensing channel, Nav1.7, and investigate the role of the VGSC β3 subunit in cancer guided by bioinformatic analysis. *In silico* docking was …
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