Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 85 for “"single-cell sequencing"”.
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Experimental Design of Single Cell Sequencing Experiments
This thesis addresses limitations of current single-cell sequencing technologies and proposes alternative experimental designs to increase statistical power. Single-cell sequencing enables high-throughput and high-dimensional studies of bio- logical systems. This is particularly useful in …
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Resolving developmental dynamics using single-cell sequencing data
… disrupted form in disease. Over the past decade, single-cell sequencing has become one of the key technologies to generate high resolution in vivo snapshots to study developmental trajectories. After giving an overview of the current state of single cell technologies and computational methods, I …
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Simultaneous SNV calling and Phylogenetic Inference for Single-cell Sequencing Data
Single-cell sequencing provides a powerful approach for elucidating intratumor heterogeneity by resolving cell-to-cell variability. However, it also poses additional challenges including elevated error rates, allelic dropout, and non-uniform coverage. Variant calling in this context is the task of …
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Combatting pathogens through directed evolution, single-cell sequencing, and synthetic immunology
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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Characterising the evolutionary dynamics of hypermutated tumours using single-cell sequencing
… evolution have relied on bulk whole-genome sequencing (WGS), which is limited to infer the clonal structure and dynamics of tumours due to the impossibility of assigning mutations to subclones and limited sensitivity for subclonal mutation detection. Thus, the evolutionary trajectories and …
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Systems and Comparative Analyses of Monocyte Dynamics Based Upon Single Cell Sequencing Data
… and dynamic responses of monocytes, crucial cells of the innate immune system. Understanding these responses, particularly to lipopolysaccharide (LPS), a key inflammatory stimulus, is vital yet remains challenging due to their heterogeneity and plasticity. Upon analyzing available single-cell …
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Genomic Evolution of Chemoresistance In Triple-Negative Breast Cancer Delineated By Single Cell Sequencing
… To investigate this question we applied single cell DNA and RNA sequencing methods and deep-exome sequencing to longitudinal time-point samples collected from a cohort of 20 TNBC patients. Deep-exome sequencing of the cohort at three time points revealed patterns of both clonal extinction …
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Genetic regulation of neuronal stem cell differentiation: a single-cell sequencing study in planarians
Cellular specialisation is incredibly prominent in the nervous system, where even in a single brain region, individual neurons may differ deeply in their molecular, anatomical, and functional features. Platyhelminthes like Schmidtea mediterranea continuously turn over all their tissues, including …
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Characterization, representation, and prediction of immune cell states using single-cell sequencing and machine learning
… yet the high-resolution assessment of immune cell states remains a significant challenge. The advent of single-cell sequencing has revolutionized this research field by enabling the profiling of individual cellular transcriptomes. However, the resulting high-dimensional datasets are frequently …
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Decoding The Tumor and Immune Microenvironment In Pdac and Breast Cancer By Single Cell Sequencing.
<p>This work utilizes single cell RNA sequencing to identify transcriptional populations and gene changes for the purpose of immune-related cancer therapies. First we have characterized the T cell populations of the normal and malignant human pancreas. Furthermore, we utilized single cell TCR …
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Methods to summarize and reduce the solution space of tumor phylogeny inference
… nature of cancer and limitations in current sequencing technology, current cancer phylogeny inference methods identify a large solution space of plausible phylogenies. To facilitate further downstream analysis, one can either summarize the set of cancer phylogenies or use additional data to …
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Exploring the transcriptomic landscape of the Drosophila melanogaster embryonic salivary gland.
… fuse to form a Y-shaped organ. Salivary gland cells do not undergo cell death or division, instead only through changing their shape, size, and neighbours. The short time window of specification, small cell population, continual lineage of cells and the established spatial patterning of genes …
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Statistical Methods For Resolving Intratumor Heterogeneity With Single-Cell Dna Sequencing
<p>Tumor cells have heterogeneous genotypes, which drives progression and treatment resistance. Such genetic intratumor heterogeneity plays a role in the process of clonal evolution that underlies tumor progression and treatment resistance. Single-cell DNA sequencing is a promising experimental …
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Utilising Single-Cell Technology to Investigate Gene Regulation
Since the inception of single-cell sequencing in 2009, experimental and computational technologies have enabled the accurate quantification of gene expression on a cell-by-cell basis. By capturing the transcriptome of thousands of single cells within complex tissues, ranging from organoids to …
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Inferring tumour evolution from single-cell and multi-sample data
… as an evolutionary process during which cells accumulate mutations and evolve into a mix of genetically distinct cell subpopulations. The resulting genetic intra-tumour heterogeneity poses a major challenge to cancer therapy, as it increases the chance of drug resistance. To study tumour …
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Eomes Directs the Formation of Spatially and Functionally Diverse Extra-Embryonic Mesoderm Derived Tissues
… embryonic development, the first mesodermal cells to originate at the posterior primitive streak will give rise to the extra-embryonic mesoderm (ExEM). These cells will contribute to key tissues supporting the development of the embryo, including the amnion, chorion, yolk-sac, and allantois. …
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Correlation-based Linking of Epigenomic Regions to Target Genes in Bulk and Single Cell Epigenomic Data
Single-cell sequencing has catalyzed a significant shift in biological modeling and hypothesis generation. In this study, we present advanced algorithms that utilize multiple modalities for peak-gene linking via correlation-based mechanisms. This approach provides a robust framework for bias …
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Multi-omic Analysis of Neurodegeneration in Alzheimer’s Disease and Related Dementias
The advent of single cell sequencing has revolutionized the granularity at which we can understand genetics and underlying cell biology. This enables us to analyze both the transcriptome and epigenome of various tissues, offering new insights into the molecular mechanisms that underlie disease such …
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Activin signaling controls a wound-induced program essential for regenerative patterning
… signaling from specific subsets of muscle cells. Furthermore, several of these subsets have been shown to express specific transcription factors, for which inhibition results in specific patterning phenotypes. Muscle heterogeneity and function in regeneration can be further studied through …
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Biomedical data sharing and analysis at scale : privacy, compaction, and integration
… biomedical datasets, including personal genomes, single-cell sequencing experiments, and molecular interaction networks. The unprecedented scale, variety, and distributed ownership of emerging biomedical datasets present key computational challenges for sharing and analyzing these data to uncover …
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