Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"single-cell gene expression"”.
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Probabilistic modelling of cellular development from single-cell gene expression
The recent technology of single-cell RNA sequencing can be used to investigate molecular, transcriptional, changes in cells as they develop. I reviewed the literature on the technology, and made a large scale quantitative comparison of the different implementations of single cell RNA sequencing to …
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Highly Constrained Kinetic Models for Quantitative Single-Cell Gene Expression Analysis
Cells are for the most part genetically identical, and it is the gene expression which determines different cell types in the body. Transcription, the first step in gene expression, is a complex, multi-step process initiated by transcription factors (TFs) binding to DNA promoter regions, followed …
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Investigating Parkinson’s Disease human iPSC-derived models through single-cell gene expression
… of use in research, induced pluripotent stem cell(iPSC) technologies are still under active development for clinical and disease modeling purposes. Directed differentiation protocols of iPSC to midbrain dopaminergic neurons enables the study human midbrain Dopamine(DA) neurons, the primary …
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Synthesising executable gene regulatory networks in haematopoiesis from single-cell gene expression data
… in biology is to understand the complex gene regulatory networks which control tissue development in the mammalian embryo, and maintain homoeostasis in the adult. The cell fate decisions underlying these processes are ultimately made at the level of individual cells. Recent experimental …
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Alendronate Alters Single Cell Gene Expression of Cortical Osteoblast Lineage Cells During Bone Loss
… it difficult to examine specific populations of cells which are integral to the tissue using traditional molecular methods. For this study we examined single cell cortical osteoblasts derived from the femurs of C57BL/6J mice. After isolating single cells from bone, we were able to individually …
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Single cell gene expression in Bacillus subtilis : an investigation into the molecular basis of culture heterogeneity
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1994.
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Single-Cell Gene Expression Variation as A Cell-Type Specific Trait: A Study of Mammalian Gene Expression Using Single-Cell RNA Sequencing
In this dissertation, we used single-cell RNA sequencing data from five mammalian tissues to characterize patterns of gene expression across single cells, transcriptome-wide and in a cell-type-specific manner (Part 1). Additionally, we characterized single-cell RNA sequencing methods as a resource …
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Single-cell approaches reveal functional and molecular heterogeneity in malignant haematopoietic stem cells
Recent advances in single-cell technologies have permitted the investigation of heterogeneous cell populations at previously unattainable resolution. In this thesis single-cell approaches are applied to resolve the molecular mechanisms driving disease in mouse haematopoietic stem cells (HSCs), …
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Algorithms for regulatory network inference and experiment planning in systems biology
… problems that arise in the study of complex cellular processes. The first problem arises in the context of planning large-scale genetic cross experiments that can be used to validate predictions of multigenic perturbations made by mathematical models. (i) I present CrossPlan, a novel …
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Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm
… with diabetes. Transplantations of islet cells are an alternative treatment that could help patients to become insulin independent and presumably also delay the development of complications. However, the increasing number of diabetic patients would require large amounts of transplantable …
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Enrichment and Transcriptome Analysis of CD4+ T Cell Clones Harboring Intact HIV-1 Proviruses
… that infects and destroys predominantly CD4+ T cells. Since CD4+ T cells are crucial for the innate and adaptive immune system, their progressive loss in the course of HIV-1 infection leads to the Acquired Immune Deficiency Syndrome (AIDS). Antiretroviral therapy can suppress the virus in people …
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Computational methods to dissect the genetic basis of human disease
… successful in identifying disease-associated genetic variants. However, the path from GWAS to biological insight remains challenging, notably in identifying relevant biological pathways, explaining mechanistic links between diseases, and nominating disease-critical tissues and cell types. In …
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Characterizing T Cell Features of Atypical Lymphocytes for the Optimization of Adoptive Immunotherapies
… results. Two such populations are regulatory T cells (Tregs) and CAR (chimeric antigen receptor-modified) T cells. The functional role of Tregs in the immune system is non-canonical, in that they prevent hyper-inflammation and resolve immunopathology by constraining conventional immune cell …
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Mapping the Feeding Circuitry in the Mouse and Human Brain
… improve prevention and treatment of obesity. Genetic studies highlight the brain as a critical centre for food intake control. In particular, the hypothalamus and the hindbrain play key roles in the integration of signals regarding the body’s energy status and coordinating a response to alter …
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Investigating the competing endogenous RNA hypothesis genome-wide and in single cells
… endogenous RNAs or ceRNAs) has prompted a general "ceRNA hypothesis" that RNAs can regulate each other indirectly through global RNA-miRNA-RNA networks. These ceRNAs are said to "crosstalk" with each other by competing for common miRNAs. Although many individual ceRNAs have been found, …
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Modeling temporally-regulated effects on distributions
… Our methods are motivated by the recent rise of single-cell RNA-sequencing time course experiments, in which an important analytic goal is the identification of genes relevant to the progression of a biological process of interest at cellular resolution. As existing statistical tools are not …
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Understanding machine learning models of the epigenome with statistics and statistical mechanics
Epigenetic changes are chemical and structural modifications of DNA and its associated proteins which do not change DNA sequence. These modifications mark and package DNA in different ways and help to establish cell types, which are distinct and heritable gene expression states. Understanding …
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A Single Cell and Spatial Transcriptomic Investigation of Traumatic Brain Injury: Novel insights into endothelial-derived Eph signaling
… that involve significant dysfunction at the cellular and molecular level. One major predictor of severe TBI outcome is the extent of blood-brain barrier (BBB) disruption, which under normal conditions prevents the passage of bacteria, neurotoxins, and macromolecules from entering the brain. …
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Sequence-driven gene regulation across cellular contexts
Gene expression is a highly coordinated process that depends on the interplay of the DNA and transcription factors that recognise subsequences of the DNA. Transcription factors can induce chromatin accessibility and recruit co-factor proteins, leading to the indirect recruitment of RNA polymerase …
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