Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"single-cell RNAseq"”.
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Investigation of endogenous and adoptively transferred T cell function in a genetic mouse model of lung adenocarcinoma
… other immunotherapeutic modalities like adoptive cell therapy (ACT). The reasons for these low response rates are not well understood, suggesting an improved understanding of the mechanisms which shape a tumor immune response and promote T cell dysfunction is needed. Toward these ends, we first …
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Understanding transcriptional regulation through computational analysis of single-cell transcriptomics
… average gene expression across large pools of cells. In contrast, recent technological improvements have made it possible to perform expression profiling in single cells. Single-cell expression profiling is able to capture heterogeneity among single cells, which is not possible in conventional …
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OXIDATIVE PHOSPHORYLATION IN MELANOMA BRAIN METASTASES: A REGULATOR of THE TUMOR IMMUNE LANDSCAPE
… of Wildtype (WT) and NDUFS4 KO murine melanoma cells identified increased secretion of anti-tumor cytokines after OXPHOS inhibition. Multiplex flow cytometry analysis of tumor-infiltrating lymphocytes from intracranial WT and NDUFS4 KO murine melanoma tumors further demonstrated that tumor …
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Alternative splicing and RNA editing of the Complexin C-terminus regulates neurotransmitter release in Drosophila
… significant expression level differences. Single-cell RNAseq data revealed multiple Cpx7A RNA editing variants are co-expressed, indicating editing acts stochastically to generate a range of edited Cpx proteins within individual cells. To determine if RNA editing alters Cpx7A function, I …
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Functional Studies of Stem Cells in the Parasitic Flatworm Schistosoma mansoni
… within their hosts, they possess somatic stem cells referred to as neoblasts, and they are incredibly successful parasites. Until now, there has been little appreciation for the interrelationships between the tegument, neoblasts, and successful parasitism. Our recent work, however, suggested …
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Functional and Transcriptional Heterogeneity of the Human Haematopoietic Stem Cell Pool at Steady-State and Under Inflammation
… pool of multipotent haematopoietic stem cells (HSCs), downstream of which lineage-restricted progenitors are generated. The advent of single cell technologies has changed our view of the haematopoiesis to a dynamic continuum. Understanding the early differentiation trajectories of HSCs, …
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Genomics and host response of S. Typhi infections
… pHCM2 variant and a potential virulence related single nucleotide polymorphism (SNP) was detected in ancient clades 1.2.1 and 1.1.0. All in all, this part of the thesis highlights the importance of better genetic surveillance in Latin America. In Chapter 5, I investigated the cell-specific …
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Computational methods for single cell RNA and genome assembly resolution using genetic variation
… to demultiplex and disambiguate complex data. In single cell RNAseq, problems of batch effects, doublets, and ambient RNA are each sources of noise that impede our ability to infer the functional states of cells and compare them between experiments. One new popular new experimental design …
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Immunity Against Malaria: an Atlas of the Mosquito Cellular Immune System at Single Cell Resolution
… bystanders however, and rely on both humoral and cellular innate immune divisions to defeat invading pathogens. These efforts are coordinated by hemocytes, the insect equivalent to vertebrate’s white blood cells, circulating in the hemolymph within the insects’ body cavity. Yet, hemocyte biology …
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Exogenous mesenchymal stromal cells in osteochondral repair
… early disease, a shift that is likely to include cell-based regenerative therapies. Regenerative medicine and cell therapies are poised to have a tremendous impact on the future of medicine by delivering more effective and personalised treatment than are currently available today. Mesenchymal …
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Transcriptomic profiling of human cells destined for therapeutic applications
Cell therapy is a growing field as many diseases are still untreatable. Potential applications of cell therapies include treating cancers, autoimmune disease, urinary problems, and infectious disease, rebuilding damaged cartilage in joints, repairing spinal cord injuries, improving a weakened …
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Applications of Machine Learning in Source Attribution and Gene Function Prediction
… and (2) the functional annotation of genes using single- cell RNA sequencing data. This work proposes the development of a bioinformatics pipeline tailored for identifying genomic variants, including gene presence/absence and single nu- cleotide polymorphism. This methodology is applied to …
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Investigating Parkinson’s Disease human iPSC-derived models through single-cell gene expression
… of use in research, induced pluripotent stem cell(iPSC) technologies are still under active development for clinical and disease modeling purposes. Directed differentiation protocols of iPSC to midbrain dopaminergic neurons enables the study human midbrain Dopamine(DA) neurons, the primary …
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Driver genes associated to Broad Copy Number Aberrations in solid cancers
… (aneuploidy) are intrinsic features of tumour cells and cell transformation. The chromosomal aberrations are collectively called Broad Copy Number Aberrations (BCNAs) and mainly include aneuploidy (i.e. an incorrect number of one or more chromosomes like monosomy, trisomy, or tetrasomy), and …