Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"single-cell Hi-C"”.
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Investigating mechanisms of genome folding by single-cell Hi-C
… years researchers have begun to reveal the hierarchy of mammalian DNA folding from the 10 nm fibre to the intact whole genome. One of the key approaches used is the single-cell Hi-C. My lab and I developed a single-cell Hi-C protocol that combines imaging and Hi-C processing on the same …
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SCL: A Lattice-Based Approach to Infer Three-Dimensional Chromosome Structures from Single-Cell Hi-C Data
<p>In contrast to population-based Hi-C data, single-cell Hi-C data are zero-inflated and do not indicate the frequency of proximate DNA segments. There are a limited number of computational tools that can model the three-dimensional structures of chromosomes based on single-cell Hi-C data.</p> …
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Single-molecule G-quadruplex visualisation in live cells and 3D chromatin
… enhancers, transcription factor and architectural protein binding sites. However, the roles of G4s in biology remains elusive. Whilst G4s can exist in a folded or unfolded state, methods are required to ensure that endogenous G4 detection does not perturb G4 folding dynamics. In this …
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3D Chromatin Structure and Gene Regulation: Integrated Genome Structure, Transcriptomics and Epigenetics
… enhancer–promoter communication, but bulk Hi-C data obscure single-cell variability, while sparse single-cell Hi-C limits structural resolution. This dissertation develops computational approaches to reconstruct single-cell chromatin conformations, integrate them with functional genomics, …
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Computational Methods for Integrating Microscopy with Chromatin Structures
… can be replicated and partitioned into daughter cells, and so that regulatory elements are positioned to affect their targets. Until recently, little was known about the organisation of the genome at the scale of single genes. The packaging of DNA onto nucleosomes, and the segregation of …
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Single-molecule microscopy reveals details of heterochromatin reorganisation at the onset of mouse ES cell differentiation
… important factors linked to gene expression and cell identity. Although we have come a long way in describing how chromatin folds – from the level of single nucleosomes all the way to compartments and chromosomal territories – it is still unclear what determines this structure and why it changes …