Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"single stranded DNA (ssDNA)"”.
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Single stranded DNA: A new model system for single molecule polymer dynamics
… nearly two decades, fluorescently-labeled double stranded DNA (dsDNA) has been the model system for studying single molecule polymer dynamics in non-equilibrium conditions. However, dsDNA is a semiflexible polymer with markedly different local molecular properties compared to flexible polymer …
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Analysis of Meiotic Cancer Testis Antigens and Their Contribution to Tumorigenesis
… roles in cell survival, modulating response to DNA damaging agents, and DNA replication stress mitigation respectively. More specifically, we find HORMAD1 is critical for protecting stalled DNA replication forks in LUAD. Loss of HORMAD1 leads to nascent DNA degradation, an event which is …
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1. X-ray crystallographic analyses of the gene V protein from Ff bacteriophage, a single-stranded DNA-binding protein, and its Y41 mutants 2. X-ray crystallographic analyses of several anticancer drugs and their interactions with DNA
In biological systems, DNA/RNA-protein interactions as well as drug-DNA interactions play important roles in genetic processes. A fundamental understanding of these processes requires a detailed structural knowledge of DNAs, proteins, drugs, as well as their complexes with each other. Gene V …
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Distinct Functions of POT1 in Telomere Protection and Length Regulation
<p>Telomeres are DNA-protein structures that protect eukaryotic chromosome ends from illegitimate recombination and degradation. Telomeres become shortened with each cell division unless telomerase, a reverse transcriptase, is activated. In addition to playing a protective role of chromosome ends, …
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Reassessing DNA Preservation of Ancient Material from Poulton, UK and Testing and Optimizing of CATS: a Novel Single-Strand DNA Library Preparation Technique
Sequencing of ancient DNA with low preservation is still a major challenge, despite the immense progress that the field of ancient genetic studies has made since its inception over thirty years ago. The main aim of this study was to reassess the DNA preservation of ancient medieval material from …
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SURFACE PLASMON RESONANCE IMAGING BIOSENSORS FOR THE DETECTION OF PATHOGENS AND TOXINS IN FOOD
… purification and can exploits antibodies or single-stranded DNA (ssDNA) probes for the specific detection of pathogens and toxins with high sensitivity. My research activity has been aimed at developing SPRI biosensors able to detect pathogens and toxins in food matrices in a rapid, specific …
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Architecture and assembly of maize streak virus: insights from 3D electron microscopy
Maize streak virus (MSV), circular single stranded DNA (ssDNA) virus (~2.7kb), is the causative agent of Maize streak disease, and is a devastating pathogen that causes severe crop losses to subsistence farmers in sub-Saharan Africa. MSV is transmitted by the leafhopper Cicadulina mbila, and is the …
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UNRAVELING THE MOLECULAR MECHANISM OF BRCA2-POLTETA INTERPLAY IN PREVENTING SSDNA GAP ACCUMULATION DURING DNA REPLICATION
DNA Polymerase theta (POLθ), encoded by POLQ, plays a critical role in alternative non-homologous end joining (alt-NHEJ), a backup pathway for repairing DNA double-strand breaks in the absence of homologous recombination. The synthetic lethal relationship between POLQ and BRCA1/2 has been …
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Understanding the Role of Anisotropy on the Optoelectronic and Colloidal Properties of Quantum Rods for Programmable Self-Assembly with DNA Origami
… properties of quantum rods, as well as using DNA-mediated self-assembly to align these quantum rods on DNA origami for energy transfer applications in biocompatible systems. First, CdSe of systematically varied aspect ratios and emission colors, as well as core/shell CdSe/CdS of different …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
In the last 15 years, single molecule techniques have allowed us to observe the transient, heterogeneous, and multi‐step behavior of biomolecules often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism …
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Fluorescence imaging of single molecule dynamics on long single stranded DNA
Recent advances in single-molecule fluorescence imaging techniques have allowed the direct observation of protein dynamics on DNA, but the progress has been largely limited to double-stranded DNA (dsDNA) or short single-stranded DNA (ssDNA). Here, a single molecule imaging approach for observing …
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Exploration of helicase mechanisms at single molecule level: answering decades old questions and bioengineering super proteins
In the last 15 years, single molecule techniques have allowed us to observe the transient, heterogeneous, and multi‐step behavior of biomolecules often averaged out and lost in ensemble assays. Helicases form a ubiquitous class of enzymes that function in many aspects of nucleic acid metabolism …
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Dynamics of DNA Breathing and Folding for Molecular Recognition and Computation
… development of the molecular beacon, as a new DNA probe for DNA genotyping, DNA computation and biophysical studies of DNA conformations. Molecular beacons are single-stranded DNA molecules that form a stem-and-loop structure. A fluorophore and a quencher are grafted at their two ends to report …
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1, Structural and Functional Studies of Human Replication Protein A; 2 DNA Damage Responses and DNA Repair Defects in Laminopathy-Based Premature Aging.
… of mammalian cells is under constant attack from DNA-damaging agents. To maintain genomic integrity, cells activate an array of pathways primarily consisting of DNA repair and DNA damage checkpoints. Human replication protein A (RPA), a single-stranded DNA (ssDNA) binding protein, is essential for …
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Structural and Biochemical Investigation of the Molecular Mechanisms of DNA Response and Repair in Humans and <em>Escherichia coli</em>.
… both endogenous and exogenous agents that damage DNA. In order to maintain genetic integrity a variety of response pathways have evolved to recognize and eliminate DNA damage. Replication protein A (RPA), the eukaryotic single-stranded DNA (ssDNA) binding protein, is a required factor for all …
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Using Synthetic Nanopores for Single -Molecule Analyses: Detecting SNPs, Trapping DNA Molecules, and the Prospects for Sequencing DNA
This work focuses on studying properties of DNA molecules and DNA-protein interactions using synthetic nanopores, and it examines the prospects of sequencing DNA using synthetic nanopores. We have developed a method for discriminating between alleles that uses a synthetic nanopore to measure the …
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A label-free, fluorescence based assay for microarray
DNA chip technology has drawn tremendous attention since it emerged in the mid 90 s as a method that expedites gene sequencing by over 100-fold. DNA chip, also called DNA microarray, is a combinatorial technology in which different single-stranded DNA (ssDNA) molecules of known sequences are …
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Correlation Force Spectroscopy for Single Molecule Measurements
… (1) microrheology of Newtonian fluids and (2) single molecule force spectroscopy. To understand the correlation in thermal fluctuations of two cantilevers I initially validated the theoretical approach for analyzing correlation in terms of deterministic model using the fluctuation-dissipation …
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Expression and characterization of recombinant human NEIL3 from Escherichia coli
The DNA glycosylase NEIL3 is one of a family of proteins that release oxidized bases from DNA, thereby initiating base excision repair. NEIL3 gene expression is normally tightly regulated and expressed only in certain rapidly dividing cells, however, human NEIL3 (hNEIL3) is also highly expressed in …
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MicroCantilever (MC) based nanomechanical sensor for detection of molecular interactions
… self-assembly of alkanethiol, hybridization of ssDNA, and the formation of cocaine-aptamer complexes were measured. Dissociation constant (Kd) for each molecular reaction was utilized to estimate the surface coverage of affinity complexes. In the cases of DNA hybridization and cocaine-aptamer …
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