Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"single particle tracking (SPT)"”.
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Exploring the relation between the intracellular fate and biological activity of nucleic acid nanoparticles
… beeldvorming of het recent ontwikkelde Single Particle Tracking (SPT) die de intracellulaire processen in een volledige cel in beeld brengen. Er wordt verwacht dat de combinatie van conventionele microscopie met geavanceerde microscopie systemen zoals FCS en SPT, elk met hun specifieke …
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Direct Single Molecule Imaging of Enhanced Diffusion for Enzymes and Enzyme-conjugated Dna origami
… into this process of enhanced diffusion using single-particle tracking (SPT) with total internal reflection fluorescence (TIRF) microscopy. We found that the mobility of individual enzymes was enhanced three-fold in the presence of the substrate, and the motion remained Brownian. We showed that …
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Three-Dimensional Single Particle Tracking on the Two -Photon Microscope
We have developed a 3D single-particle-tracking (SPT) system based around the two-photon laser-scanning fluorescence microscope that can track particles in all three dimensions and at a high frequency response. We have implemented two different techniques to achieve this goal. Both techniques …
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Characterization of CEACAM1 Spatial Distribution, Self-association and Dynamics using High-resolution Microscopy Techniques
… and mutants. We have further explored the use of single particle tracking (SPT) and mean square displacement analysis to characterize CEACAM1 dynamics as a function of oligomeric state and location. Building on these datasets, we have applied co-localization and co-occurrence approaches to measure …
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Development of Three-Dimensional Super-Resolution Imaging Using a Double-Helix Point Spread Function
Single-molecule localisation microscopy (SMLM), has allowed for optical microscopy to probe biological systems beyond the diffraction limit. The intrinsic 3D nature of biology has motivated the development of 3D-SMLM with novel techniques, including the double-helix point spread function (DHPSF). A …
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Super-resolution imaging of glutamate receptors trafficking at live synapses
… previous studies were based on two-dimensional single particle tracking; lacking information on receptor movement in the z direction. Further more, the postsynapses were imaged with conventional fluorescent microscopy with diffraction-limited resolution. As a result, the detail localization of …
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Single quantum dot tracking for quantitative molecular imaging
… the past few decades has advanced the field of single-molecule biology by allowing for long time-scale tracking of individual biomolecules. However, QD single-molecule imaging studies have almost been exclusively limited to the extracellular space, due to limitations in intracellular delivery …
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Physical properties of the plasma membrane studied by local probe techniques
… waren die Rasterkraftmikroskopie (RKM) und ´single-particle tracking’ (SPT). Die RKMermöglicht die Abbildung von Ober‡ächen mit hoher Au‡ösung in physiologischer Umgebung, so lange die Wechselwirkungskräfte die Probenober‡äche nicht zu sehr deformieren. SPT ist eine Technik, um die Bewegung …
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USING SINGLE VIRION FUSION ASSAY TO STUDY INFLUENZA VIRUS ENTRY
… release viral RNA into the cytosol of the cell. Single particle tracking (SPT) enables the study of viral binding and membrane fusion at the single-virion level to obtain high resolution measurements of each of these processes in vitro. SPT combines total internal reflection fluorescence (TIRF) …
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Fluorescence lifetime imaging microscopy to study protein aggregation in the context of neurodegenerative diseases
… (ALS). Through the combined use of FLIM and single particle tracking (SPT), a tool to infer intracellular FUS viscosity in live cells, without the introduction of an external sensor, is created. Moreover, it is identified that ALS- associated mutants, P525L and R495X exhibit loss of …