Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"single nucleus RNA sequencing"”.
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Towards a complete human cell atlas: a single-nucleus RNA sequencing study of the paediatric and adult human brain
… are no studies exploring the paediatric brain at single cell resolution. To address this, we present the first paediatric brain cell atlas comprising of 6 single nucleus RNA sequencing (snRNA-seq) datasets generated from antemortem human brain tissue samples obtained during elective surgeries to …
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Single-cell transcriptomics of the mouse thalamic reticular nucleus
The thalamic reticular nucleus (TRN) is strategically located at the interface between the cortex and the thalamus, and plays a key role in regulating thalamo-cortical interactions. Current understanding of TRN neurobiology has been limited due to the lack of a comprehensive survey of TRN …
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Mapping the Feeding Circuitry in the Mouse and Human Brain
… To map the mouse hindbrain, we performed single nucleus RNA sequencing on hindbrain samples from mice fed *ad libitum* and mice subjected to an overnight fast. Here, we profile 16,034 single nuclei, and demonstrate the oligodendrocytes are transcriptionally sensitive to an overnight fast. …
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Postnatal specialization of astrocyte regional heterogeneity in the mammalian brain and improved tools for studying glia
… To address existing knowledge gaps, we used single-nucleus RNA sequencing to characterize the molecular diversity of brain cells across six developmental stages and four brain regions in the mouse and marmoset brain (Chapter 2). Using this transcriptomic atlas, we show that astrocyte regional …
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Investigating gene expression differences in acute and organotypic mouse hippocampal brain slices as models for studying the brain
… slices remains poorly characterized. Here, using single-nucleus RNA sequencing, I performed a comprehensive comparison of gene expression between OBSCs and acute brain slices prepared from postnatal day 7 mouse hippocampus. I identified significant differences in cellular composition, with OBSCs …
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Using single nucleus RNA-seq to determine the effect of immunosuppression on the transcriptional activity of resident CNS cells in a mouse model of CNS-TB
… response to CNS-TB, but none have done so atsingle cell resolution. Single nucleus RNA Sequencing (snRNA-seq) was used to determine the effect of immunosuppression on the transcriptional activity of resident CNS cells in a mouse model of CNS-TB. The model consisted of two mouse genotypes, …
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Heterogeneity in the Pitx2ON Population in the Mouse Superior Colliculus
… within Pitx2ON neurons, starting with single-nucleus RNA sequencing to assess molecular diversity. Differential expression of transcription factors, neurotransmitter receptor genes, and canonical markers suggests a molecularly heterogeneous population. Complementary viral tracing …
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Hdac6 Inhibition Reverses Long-Term Doxorubicin-Induced Cognitive Dysfunction By Restoring Microglia Homeostasis
… staining. Using advanced imaging techniques and single-nucleus RNA sequencing, I demonstrate that doxorubicin-induced changes are associated with decreased microglial ramification and alterations in the microglia transcriptome that suggest a neurodegenerative microglia phenotype closely …
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Μεταγραφωματική και επιγενετική ανάλυση μονήρων κυττάρων ασθενών με καρκίνο του πνεύμονα
… τεχνολογίες αλληλούχισης μονήρων κυττάρων (single-cell multiomic technologies) επιτρέπουν την ταυτόχρονη χαρτογράφηση της γονιδιακής έκφρασης και των επιγενετικών μηχανισμών σε μεμονωμένα κύτταρα. Στην παρούσα μελέτη αναλύθηκαν δεδομένα single-nucleus RNA sequencing και single-nucleus ATAC …
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Dopamine-dependent Transcriptional Dynamics in Striatal Physiology and Cocaine Reward
… that may contribute to addiction. The nucleus accumbens (NAc), part of the mesocorticolimbic dopamine pathway, plays a central role in motivation, reward, and reward-related learning, and this brain region is highly implicated in the development and maintenance of addiction. However, …
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Transcriptional Regulation of Neonatal Heart Regeneration and Direct Cardiac Reprogramming
… the mouse heart following injury, we performed single cell RNA-sequencing on neonatal hearts at various timepoints following myocardial infarction, and coupled the results with bulk tissue RNA-sequencing and H3K27ac ChIP-sequencing data collected at the same timepoints. This approach provides …
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The Aging-Induced Decline in Beige Adipose Tissue Development
… observe remarkably similar ASPC populations by single cell RNA-sequencing between ages, leading to the conclusion that the defect in de novo beige adipogenesis is likely cell non-autonomous. Using single-nucleus RNA-sequencing to transcriptionally profile adipocytes, we identify four adipocyte …
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Transcriptional Heterogeneity in ALS: Patients with Retrovirus-mediated Disease and Potential Cell-type Specific Therapeutic Approaches
… most clinical trials treat all ALS patients as a single group. The hypothesis addressed in this thesis is that ALS is a transcriptionally heterogeneous disease with distinct biological pathways converging on a similar phenotype. To address this hypothesis, a bulk RNA sequencing (RNA-seq) dataset …
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Profiling the dynamics of the active transcriptome in the juvenile and adult brain using spatial transcriptomics
… various cell types. Previous studies have used single-cell/single nucleus RNA-sequencing (sc/snRNA-seq) to provide vital information on gene expression at a cell-specific level. These studies, which focused on either the pre-natal or adult brain, lacked comprehensive information about the …
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The molecular, hormonal and symptomatic responses to hypoglycaemia in mice
… or hyperglycaemia. Therefore, I aimed to use single-nucleus RNA sequencing (snRNAseq) to reveal the hypothalamic molecular signatures and gene expression profiles of different blood glucose levels. Hyperinsulinaemic clamps were used to create carefully controlled hypoglycaemic, euglycaemic and …
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Spontaneously Regenerative Corticospinal Neurons in Mice
… tracing methods, tissue clearing, and Smart-seq3 single-nucleus RNA-sequencing (snRNA-seq), we found that ipsilateral CSN axons project to regions of the ventral horn, including directly to motor neurons. Barcode-based Multiplexed Analysis of Projections by Sequencing (MAPseq) of the CST revealed …
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Transcriptomic Signatures of Projection Class Neurons for Vocal Control
… approach, in combination with long-read sequencing technologies, to produce a diploid genome with the two parental haplotypes assembled independently. This method captures the full range of heterozygous variations at high rates of accuracy between parental alleles in this species that …
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Development Of Single-Cell Transcriptomic & Epigenomic Sequencing Technologies To Assess Cell-Type-Specific Gene Regulatory Programs In Mammalian Brains
Recent efforts to develop single-cell technologies have played a pivotal role in our ability to gain a high-resolution understanding of how tissue heterogeneity impacts human health, development, and disease. These technologies have become so powerful and popular in such a short period of time, and …
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Evolution of a Novel Neuronal Sex-Specifically Spliced Gene in Mosquitoes
… we take advantage of available sex-specific bulk RNA sequencing and single nucleus RNA sequencing data across mosquito tissue to characterize the expression pattern of <em>AAEL011211</em> in <em>Aedes aegypti</em>. We find that it is enriched in the nervous system and is neuron-specific, …
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