Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 90 for “"single molecules"”.
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Theory of electron transport through single molecules
The theoretical work carried out in this thesis presents the electrical properties of two different types of two terminal nanojunctions: one dealing with gold electrodes which form gold|molecule|gold structures and the other has carbon nanotube (CNT) electrodes forming CNT|molecule|CNT junctions. …
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Ensemble and single molecules fluorescence studies of polymers
… (PFO) are investigated at both single molecule and ensemble levels. Single molecule studies reveal the conformation of PFO chains to be more globular when cast from THF than from toluene. Intensity transients of single molecules show that the elongated molecules cast from toluene …
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Stochastic Modelling of Gene Expression: From Single Molecules to Populations
… sequence of DNA are transformed into functional molecules, namely proteins and RNA chains. These molecules and the intricate network of interactions among them are the driving force behind most cellular processes, including gene expression itself. Also, of particular importance is the regulation …
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Development of single-molecules techniques to study native α-Synuclein aggregates
… (<5% of a solution of protein). For this reason, single-molecule fluorescence microscopy has been used to study them; by probing molecules one-by-one, the rare oligomeric species can be identified amongst the monomeric protein, counted and characterised. Typically, to study species in …
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Light and Single-molecule Coupling in Plasmonic Nanogaps
… modes to probe and manipulate the dynamics of single-molecules at room temperature. In this thesis, nanogap cavities are constructed by placing nanoparticles on top of a metal-film separated by molecular spacers. Such nanogaps act as cavities with confined optical fields in the gap. Precise …
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DEVELOPMENTS TOWARDS SINGLE MOLECULE AND SINGLE CELL GENETIC AND EPIGENETIC ANALYSIS TECHNOLOGIES
… state and progression, thus, research into single cells and single molecules are a necessity to improving treatment. In this work, methods of single molecule analysis of native human chromatin fibers are presented as well as methods and devices for multi- and single cell genome analysis. As …
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Chemical Nanolithography and Single Molecule Optical Imaging With Metal -Coated Probes
… features, labeled with fluorescent dyes, biomolecules, and nanoparticles, were imaged with confocal microscopy and atomic force microscopy. The use of metal-coated AFM tips in apertureless near-field scanning optical microscopy of single molecules is also briefly discussed.
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Structural Organization and Chemical Activity Revealed by New Developments in Single-Molecule Fluorescence and Orientation Imaging
<p>Single-molecule (SM) fluorescence and its localization are important and versatile tools for understanding and quantifying dynamical nanoscale behavior of nanoparticles and biological systems. By actively controlling the concentration of fluorescent molecules and precisely localizing individual …
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Charge Transport in Organic Electronic Devices: From Nano to Macro-Scale
… compound result from the structure of the molecules, their solid-state packing, efficiency of charge injection from the electrodes, and the fabrication procedures. The length scales of interest can also vary widely, ranging from a few nanometers in the case of charge transport through …
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The ATP synthase from spinach chloroplasts (Spinacea oleracea) : improved isolation and purification of the enzyme and conformational changes observed with fluorescent labels
… of CF1 labelled with Alexa 546 was measured on single molecules using FCS. The translational diffusions coefficient was increased in the presence of AMP-PNP. This was interpreted as a reduction in volume of the enzyme upon substrate binding and demonstrated that conformational changes of CF1 …
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An analysis of exciton transport, electron tunneling, and electromigration in nanotube and nanowire systems
… in metal nanowires, electron tunneling in single-molecules, and carbon nanotube photovoltaics - are investigated. In the first area, electromigrative failure of metal nanowires has been shown to form single-molecule tunnel junctions, but the process has remained unpredictable, limiting the …
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DNA ruler : enhancing nanopore sizing resolution by multiple measurements on the same DNA molecule
… versatile sensors for label-free detection of single molecules and particles that have attracted attention for applications such as DNA sequencing and nanoparticle analysis. Detection of single molecules or particles in nanopores is enabled by monitoring the ionic current through the pore: …
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Microfluidic-based Fluorescence Detection for Biomarker Analysis
… With the 8.8cm channel and herringbone features, Single CTCs can be collected with less than one contaminating WBC, making sequential eDAR (S-eDAR) amenable to downstream analyses such as single-cell sequencing and single-cell fluorescence in situ hybridization (FISH). S-eDAR was further applied …
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FUNCTIONAL REDOX-ACTIVE MOLECULAR TUNNEL JUNCTIONS
Molecular electronics, which use single molecules or self-assembled monolayers as active electrical elements, are complementary to semiconductor-based electronics with the ultimate goal of reducing the footprint or energy consumption of the electronic devices or uncovering new functionalities for …
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Towards the rational design of mechanical proteins
… have made possible tools to directly manipulate single molecules mechanically. The atomic force microscope is a flexible and robust platform that allows us this ability. At the same time computational tools have become increasingly common companions and facilitators of theoretical and …
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The promise and perils of molecular combing
… Combing (DMC) is a method of making visible single molecules of DNA. The method stretches the molecules, attaching them to a glass coverslip; labels them with an intercalating dye or fluorescent antibodies; and images the molecules with fluorescent microscopy. After combing, fluorescent in …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
Single-molecule super-resolution fluorescence microscopy is a powerful method for imaging detailed biological structures at the nanoscale. However, imaging capabilities in thick samples such as mammalian cells remain limited due to increased fluorescence background which degrades the achievable …
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
… depletion (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. Being ideal point-like probes, single molecules can therefore …
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Intracellular labeling of live cells via transient membrane permeabilization for fluorescence and super resolution microscopy
… Furthermore, we show the ability to image single proteins inside the living cell by tracking single molecules of kinesin using photostable probes.
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Nanoscale control in biological and synthetic systems
… level. Indeed, methods for manipulating single molecules have in some ways outstripped the ability to observe the systems under study. The “computational microscope” of the molecular dynamics (MD) simulation method provides insight into the behavior of systems at the atomic level, …
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