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Showing 1 to 16 of 16 for “"single molecule tracking"”.
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Single molecule tracking studies of lower critical solution temperature transition behavior in poly(N-isopropylacrylamide)
In this thesis project, single molecule tracking, SMT, experiments in poly(n-isopropylacrylamide, pNIPAAm, were carried out using probe fluorophores that had partitioned into the polymer. When the pNIPAAm switches from expanded to collapsed at elevated temperatures, the free volume accessible to …
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Development of the single-molecule tracking and fluorescence lifetime techniques for biophysical measurements and biomedical applications
Single-molecule detection allows us to reveal the biophysical features of rare species in complex samples. Direct characterization of annealing/melting kinetics of nucleic acids without the need for synchronization of molecular states can be achieved with single-molecule detection, but the current …
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Single molecule tracking studies of solvent-swollen microdomains in cylinder-forming polystyrene-Poly (ethylene oxide) diblock copolymer films
… oxide) (PS-b-PEO) films is done by using single molecule tracking. These films are prepared by sandwiching benzene (with/without methanol) or THF (with/without methanol) solutions containing 5 nM sulforhodamine B (SRB) between two glass substrates. The PEO microdomains are aligned in the …
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Particle Filtering for Continuous Time Problems
… to parameter inference in a challenging 3D single molecule tracking problem using a Born and Wolf observation model. A second key contribution is the novel coupling construction of conditional particle filters (CPFs) using ``single event’’ killing resampling. While this approach introduces …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
… pluripotency through to lineage specification. Single-molecule tracking of pluripotency-associated TFs in live mouse ESCs revealed that remodelling by NuRD acts to actively promote the dissociation of these TFs from chromatin, limiting their residence times. The regulation of TF occupancy …
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Smart, biocompatible semi-conductor nanocrystal constructs designed for in-vitro imaging applications
… by exploring a new method to conjugate molecules onto the QD surface. The improvements that were made enabled a collaborator situated across the country to conjugate biomolecules in a one-step process without performing the usual amine/N-hydroxysuccinimide coupling, thereby diminishing …
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Dynamics and Turnover of Lipid and Protein Domains in Cells and Supported Bilayers
… measured using dynamic measurements (FRAP and single molecule tracking), first by looking at substrate dye interactions to ensure dynamics were not altered by unintended interactions between dye and substrate. Following these studies it was determined that the commonly used poly-L-lysine …
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Biophysical methods to study the ubiquitination of amyloidogenic proteins and proteasomal responses to protein aggregates
… stress, some foci co-localised with aggregates. Single molecule tracking experiments indicated these proteasome-containing bodies act as active proteolytic centres to provide cells with an extra defence function against stress. In addition, a photoconvertible Ub construct enables the direct …
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Applying single-molecule localisation microscopy to achieve virtual optical sectioning and study T-cell activation
Single-molecule localisation microscopy (SMLM) allows imaging of fluorescently-tagged proteins in live cells with a precision well below that of the diffraction limit. As a single-molecule technique, it has also introduced a new quantitative approach to fluorescence microscopy. In the Part A of …
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Next-generation fluorophores for single-molecule and super-resolution fluorescence microscopy
The development of single-molecule and super-resolution fluorescence techniques has revolutionised biological imaging. Nano-scale cellular structures and heterogeneous dynamic processes are now able to be visualised with unprecedented resolution in both time and space. The achievable localisation …
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Investigations into the dynamic uptake of extracellular vesicles and their role in breast cancer metastasis after chemotherapy
… membrane interactions, thus affecting EV uptake. Single molecule tracking revealed two patterns of such interactions: short and fast, and long and slow. To study the effect of EVs after chemotherapy, docetaxel and mitomycin C, two anti-cancer agents used for the treatment of breast cancer, were …
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3D tracking of solute dynamics in heterogeneous polymer networks
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms
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Biomacromolecular assemblies: from quinary interactions to diffusion in mammalian cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Super-resolution Microscopy: Novel Developments and Optimisations
… Centre. The microscope is optimised to perform single and dual-colour imaging with high localisation precision and accuracy. Moreover, three-dimensional imaging capability is included in the microscope using the double-helix point spread function and the light field imaging modality. The thesis …
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Cell squeezing : a vector-free microfluidic platform for intracellular delivery of macromolecules
… quantum dots, carbon nanotubes, and small molecules. Our cell squeezing technology has further illustrated its enabling potential in a number of applications detailed herein. Quantum dots are a promising alternative to organic fluorescent dyes due to their superior spectral properties and …