Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"single molecule spectroscopy"”.
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Aggregation of alpha-synuclein using single-molecule spectroscopy
… conditions. In this thesis, well-established single-molecule techniques have been utilised to study the aggregation and oligomerisation of αS in solution.
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
… depletion (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. Being ideal point-like probes, single molecules can therefore …
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Time resolved fluorescence of CdSe nanocrystals using single molecule spectroscopy
… conditions. In particular the methods of single molecule spectroscopy are used in many experiments allowing the behavior of individual NCs to be resolved from the blurring effect of averaging over the ensemble. Studies of the excited state lifetime of band edge fluorescence from single NCs …
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Theoretical study of single-molecule spectroscopy and vibrational spectroscopy in condensed phases
… are employed to study several problems of single-molecule spectroscopy and vibrational spectroscopy in condensed phases. The first part of the thesis concentrates on studying dynamic disorders probed by single molecule fluorescence spectroscopy. Event statistics and correlations of …
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Advances in single molecule spectroscopy and microscopy for biological imaging and polymer characterization
Single molecule fluorescence microscopy and single molecule spectroscopy provide tremendously powerful methods for studying the behavior of a wide variety of biological systems. In this way, single molecule techniques can be used to gain an increased understanding of molecular mechanisms underlying …
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Dissecting nuclear protein dynamics and structural changes with single-molecule spectroscopy and microscopy
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01
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Interactions of light with matter-- applications to single molecule spectroscopy and quantum control
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1998.
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Examining Post-Translational Modifications In Alpha-Synuclein Through Semi-Synthesis And Single Molecule Spectroscopy
… effects for arginylation at more than one site. Single molecule Förster resonance energy transfer (smFRET) measurements reveal changes in monomer conformation that underlie a possible mechanism for the differential aggregation profile of phosphorylated αS. In addition to its disease-relevant …
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Dynamics and spectra in condensed phases : single molecule spectroscopy, electron transfer, and excitation energy transfer
… main topics. First, a theoretical description of single molecule spectroscopy is presented in order to study time-dependent fluctuations of single molecule spectra in a dynamic environment. In particular, the photon counting statistics is investigated for a single molecule undergoing a generic …
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Synthesis and characterization of transparent conducting oxides for Stark e ect applications in single molecule spectroscopy
… oxides (TCOs) for Stark application in single molecule spectroscopy (SMS) stemmed from our interest in the dark states of light harvesting complex II (LHCII) of plants. Investigation of this mechanism requires a Stark cell. The high optical quality required by the SMS procedure demands a …
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The Structure of the RC-LH1 Complex from Rps. acidophila: Optical Single-Molecule Spectroscopy and Numerical Simulations
… arrangement of the bacteriochlorophyll (BChl) a molecules in the light-harvesting 1 (LH1) complex from Rhodopseudomonas (Rps.) acidophila. In purple bacteria the LH1 complex forms an interconnected unit with a reaction-center (RC) complex, where the LH1 complex directly surrounds the RC to form …
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Investigation of the Photophysical Properties of pi-Conjugated Polymers. A Study by Non-Linear, Time-Resolved, and Single-Molecule Spectroscopy
… by two-photon (2P) fluorescence excitation spectroscopy and time-resolved spectroscopy on thin MeLPPP-films as well as by fluorescence spectroscopy on individual MeLPPP-chains. Employing continuous-wave 2P fluorescence-excitation spectroscopy on MeLPPP the symmetry properties of the vibronic …
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Donor-Sigma-Acceptor (D-sigma-A) Molecules With Perylenebisimide Acceptors Having Alkyl or Peg Swallowtails With Sulfur or Hydroxyl Anchors: Synthesis and Applications in Langmuir-Blodgett Films, Single Molecule Spectroscopy (SMS), G-Quadruplex Studies and Passivation of Gold Nanoparticles
<p>Use of single molecules as electronic devices is presently considered a potential alternative to semiconductor-based nanoscale electronics. The ability to study a single molecule or a small group of molecules provides a great opportunity to build electronic devices at the molecular level. It is …
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Pigment-Pigment Interactions and Protein Dynamics in Light-Harvesting Complexes: a Single-Molecule Study
… were studied with low-temperature optical single molecule spectroscopy. In one series of experiments the spectral properties of the bacteriochlorophyll a molecules within the complexes were studied in the view of model systems of molecular aggregates. It was found that the excitations in …
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Studies on the different types of LH2 complexes from the purple non-sulphur photosynthetic bacterium Rhodopseudomonas palustris strain 2.1.6
… composition exist has been addressed by single-molecule spectroscopy. For the first time, direct evidence that individual LL LH2 complexes have a heterogeneous αβ-apoprotein composition has been found. Such mixed rings feature Bchl a molecules with both B820-like and B850-like …
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Optically-Assisted Scanning Tunneling Microscopy
… STM promises to become a powerful technique for single molecule spectroscopy, enabling one to examine the response of single molecules on a surface. Atomic scale laser-assisted STM has thus far remained an elusive goal, with few recent experiments reaching sub-nanometer resolution. This is due to …
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Single molecule studies of rna-target interactions
<p>We have used FRET, single molecule spectroscopy, and several other biophysical techniques to study how the folding of RNA allows it to perform its various functions by recognizing and binding a target ligand. We have shown that the c-di-GMP riboswitch undergoes a large, global conformational …
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Dynamics of Hairpin Ribozyme
This thesis research uses single molecule spectroscopy to study the dynamics of hairpin ribozyme---an enzymatic RNA molecule capable of cleaving phosphodiester linkage. RNA controls key processes of gene expression. Ribozymes can catalytically edit precursor RNA message transcribed from DNA, the …
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Understanding the activation-deactivation mechanism of glutamate receptor proteins at the single molecule level
… the ion channels are controlled by a number of molecules (ligands) interacting with specific binding sites. Hence, these binding sites can serve as potential drug targets for controlling the aberrant activation of the ion channel that leads to a number of neurological disorders. In this …
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On transient assemblies in amyloid formation: mechanistic insights and therapeutic strategies
… Through a combination of kinetic assays and single-molecule spectroscopy, I demonstrate that α-Synuclein oligomers form through secondary nucleation under physiologically relevant conditions. Using Amyloid-β as a model system, I then examine the impact of mechanical forces on amyloid …
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