Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"single molecule force spectroscopy"”.
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Synthesis and single-molecule force spectroscopy of supramolecular capsules
… cage in ESI-MS. The encapsulation of guest molecules in the supramolecular assembly will be in the focus of future studies. In the second part of this work, the interactions in a hydrogen-bonded assembly have been studied using single-molecule force spectroscopy to evaluate the dynamics of …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
Single-stranded DNA binding (SSB) proteins are essential accessory proteins that protect single-stranded DNA (ssDNA) during genome maintenance. Escherichia coli SSB is a prototypical homo-tetrameric SSB protein that can wrap up to 65 nucleotides of ssDNA in one of its binding modes. Here we present …
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Probing static disorder in protein unfolding and chemical reactions by single-molecule force spectroscopy
… of biomolecular reactions under mechanical force, including protein unfolding and disulfide-bond reduction, probed at the single-molecule level. The advent of single-molecule force spectroscopy has allowed the direct measure of force-dependent reaction rates, providing a powerful approach to …
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Each one teaches one: characterizing active forms of proteins by single molecule force spectroscopy
… deriving from the action of mechanical forces in vivo (Chapter 3) and the complexity of conformational equilibria in intrinsically unstructured proteins and amyloid formation (Chapter 4). My experimental work investigated both these examples by using in both cases the single molecule …
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Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
Mechanical forces play an important role in cell morphology, orientation, migration, adhesion and can even induce apoptosis. The eukaryotic cell is equipped with a dynamic frame, known as the cytoskeleton, that provides the cell's structural integrity in order to sustain and react to such forces. …
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Mechanical Processes of a Single Synthetic Molecular Machine Studied by AFM-based Force Spectroscopy
Some biomolecules are able to generate directional forces by rectifying random thermal motions. This allows these molecular machines to perform mechanical tasks such as intracellular cargo transport or muscle contraction in plants and animals. Although some artificial molecular machines have been …
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An automated and user-friendly optical tweezers for biomolecular investigations
… application. Fi- nally, I present new results of single-molecule force spectroscopy of DNA overstretch- ing as a function of water isotope type. Single DNA unzipping is also presented as a proof of concept.
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Escape processes far from thermal equilibrium : path integrals and force spectroscopy
… of paramount importance. For example, single-molecule force spectroscopy experiments, and a number of other physical systems are governed by thermally activated transitions out of a metastable state under the action of a steadily increasing external force. This allows to observe the …
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Critical role of histone tail entropy in nucleosome unwinding
… while keeping the DNA accessible to protein molecules. Using a transferable protein-DNA model and advanced sampling techniques, we investigated the stability and dynamics of the nucleosome by determining the free energy cost of its DNA unwinding. Simulation results quantitatively reproduce …
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Structural Basis of the Tensile Strength of Protein Complexes Mediating Cell Adhesion
… behaviour of adhesive complexes of cell adhesion molecules undergoing forced detachment. Molecular-forces measurements combined with Steered Molecular Dynamic (SMD) simulations were used to investigate the mechanical response of the CD2 C58 and hemophilic C-cadherin bonds. The CD2-CD58 adhesive …
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Observing molecular interactions that determine stability, folding, and functional states of single Na+/H+ antiporters
… severe heart diseases in humans. Here I applied single-molecule force spectroscopy to investigate molecular interactions determining the structural stability of the sodium/proton antiporter NhaA of Escherichia coli, which serves as a model system for this class of proteins. Mechanical pulling of …
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Mechanochemical Reaction Development for Addition, Elimination, and Isomerization
… is now well appreciated that coupled mechanical forces can influence the rates and outcomes of covalent chemical reactions in isolated polymers and in bulk polymeric materials. Mechanochemical reactions have been widely used in mechanocatalysis, release of small molecules and protons, biasing and …
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Mechanical unfolding of membrane proteins captured with single-molecule AFM techniques
Atomic force microscopy (AFM) is a powerful technique that enables to study biological macromolecules and dynamic biological processes at different scales. It is an excellent tool for imaging of biological objects under various conditions at a nanometer resolution. Force mode of AFM, so called …
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Development of a State-of-the-Art Atomic Force Microscope for Improved Force Spectroscopy
… the development of a state-of-the-art atomic force microscope (AFM) for improved force spectroscopy. Although the AFM has been used extensively in this field of research, the performance of the instrument has been limited by inefficient operation techniques, incorrect experimental assumptions, …
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Life Under Tension: Computational Studies of Proteins Involved in Mechanotransduction
… structures that produce and transform force: from mechanical motion of our muscles and bones to sound transduction and cell volume regulation, handling of forces is essential to life. Investigation of the microscopic machinery behind force generation and transduction in the cell has …
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Structural characterization of synaptotagmin I
… mechanical properties of the C2-domains using single molecule force spectroscopy.\r\nWe studied the mechanical stability of the C2A and C2B domains of human Syt1 using single-molecule atomic force microscopy. We found that stretching the C2AB domains of Syt1 resulted in two distinct unfolding …
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Correlation Force Spectroscopy for Single Molecule Measurements
This thesis addresses development of a new force spectroscopy tool, correlation force spectroscopy (CFS), for the measurement of the mechanical properties of very small volumes of material (molecular to µm³) at kHz-MHz time-scales. CFS is based on atomic force microscopy (AFM) and the principles of …
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Single molecule studies of ligand-DNA interactions using atomic force microscopy
… principally using the technique of atomic force microscopy (AFM). Since the description of the first AFM in the mid 1980’s, AFM has emerged as a sensitive and versatile analytical tool, capable both of detecting and manipulating artefacts at picometer resolutions. In these studies, AFM …
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Mechanochemical Understanding of Metal-Coordinated Polymers Using Simulation and Experiment
… experimental rheology. We then expand beyond single coordination complexes and use single molecule force spectroscopy to show that clusters of coordination bonds in model metal-coordinated protein dimers can rupture cooperatively, thereby synergistically increasing the rupture strength of the …
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