Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"single molecule fluorescence in situ hybridization"”.
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Highly Constrained Kinetic Models for Quantitative Single-Cell Gene Expression Analysis
… and it is the gene expression which determines different cell types in the body. Transcription, the first step in gene expression, is a complex, multi-step process initiated by transcription factors (TFs) binding to DNA promoter regions, followed by recruitment of proteins like chromatin …
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Microfluidic-based Tools to Investigate Gene Expression and Regulation with Tissue-specificity during Aging in Caenorhabditis elegans
Aging is a complex, universal process that impacts us all differently. An increasingly important aspect of aging research is studying gene expression and regulation. Gene expression is age-, tissue-, and individual-dependent so it is important to capture all these pieces of information. While there …
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Oligodendrocytes of the adult hypothalamic median eminence
The median eminence (ME) of the hypothalamus is a dynamic structure able to rapidly respond to nutrient availability. Both immature oligodendrocyte precursor cells (OPCs) and mature myelinating oligodendrocytes (MOLs) are found in this region in adults. While proliferation of ME OPCs has been shown …
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Microglial succinate receptor 1 (SUCNR1) sustains chronic CNS inflammation
The rapidly evolving field of immunometabolism has shed new light on new fundamental properties of metabolites, which can regulate inflammation via ligand-receptor signalling modalities. Among these, succinate signalling via succinate receptor 1 (SUCNR1) plays a key role in controlling myeloid …
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Single Cell Transcriptomics and Spatial Localization in Mouse Neurons
… signals to and from other neurons in their connected network. Though their primary functions are similar, neurons are spatially and functionally specialized across a wide array of neuronal subtypes. Characterizing the molecular contents and its variations within and across various …
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The C. elegans heterochronic pathway controls the timing of NAB/EGR-mediated terminal differentiation and the onset of adulthood
… to adult. These stages represent periods of time in which stage-specific developmental processes occur. At the end of development, the organism transitions from a juvenile state to a mature adult. The failure to undergo a specific developmental stage, or the failure to transition to an adult can …
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Mechanisms of Specificity in Neuronal Activity-regulated Gene Transcription
<p>The ability to convert sensory stimuli into long-lasting changes in brain function is essential for animals to interact with and learn from their environment. This process is achieved by encoding sensory stimuli into temporal patterns of neuronal activity, which in turn modulate the connectivity …
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Single molecule investigation of antiviral signaling
Retinoic acid-inducible gene 1 (RIG-I) plays a major role in antiviral innate immunity by detecting cytoplasmic viral RNA and triggering the pathway that leads to transcriptional activation of type I interferon (IFN). Type I IFN induces a large set of genes called interferon-stimulated genes (ISGs) …
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"Gene regulation in Escherichia coli beyond the ""rate"" approximation"
The blueprint of a living cell is inscribed in its DNA. A region of DNA encoding a protein is called a gene. The cell reads the DNA and makes molecular machines made up of proteins to carry out all cellular functions required for survival. All cells live in ever-changing environments, and have …
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Consequences of stochastic mrna synthesis in a gene regulatory pathway
… process, with elements of randomness present in both transcription and translation. This stochasticity results in cell-to-cell variation in the amounts of gene products, mRNAs and proteins, and is observed in organisms ranging from bacteria and yeast to higher eukaryotes. Randomness in the …
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Interpreting Notch signals: characterisation of dynamic transcription hubs by live imaging
The Notch signalling pathway, crucial for development, activates upon cell-to-cell contact. Despite lacking any amplification, activation of target genes is achieved robustly and efficiently after contact-induced cleavage of the Notch transmembrane protein. How relatively few molecules of the …