Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"single molecule assay"”.
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Studies on actomyosin crossbridge flexibility using a new single molecule assay.
… between these sources of flexibility, a new single molecule assay was developed to observe the swiveling of rod about a single myosin. Myosins attached through a single crossbridge displayed mostly similar torsional characteristics compared to myosins attached through two crossbridges, which …
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Mechanisms of bacterial CRISPR immunity and a phage counter-defense
… and primed acquisition, using a high-throughput single-molecule assay (Chapter 2). We also detail a protocol for fluorescently tagging CRISPR-Cas protein subunits within a complex using a sortase-mediated labeling strategy (Chapter 3). Finally, we determine how the anti-CRISPR protein AcrIIA11 …
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Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… cleavage occurs. Here, we present the single molecule fluorescence assay for probing the interaction between the ClpXP enzyme and its substrates. A covalently crosslinked ClpX hexamer maintain functionally stable form at the low concentration of single molecule level. Surface …
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Single Molecule Force Extension Measurement on Semi-Flexible Biopolymers Using Magnetic Tweezers
… using double stranded lambda-phage DNA. Single DNA molecules are studied using magnetic tweezers and an inverted fluorescence microscope. Magnetic forces in the piconewton range are applied to individual DNA molecules, and the relative chain extension due to applied force is directly …
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Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
… of the cytoskeleton Here we present a novel, single molecule assay to test the rupture force of a complex formed by an actin binding protein (ABP) linking two actin filaments. We readily demonstrate the adaptability of this assay by testing it with two different ABPs: filamin, a crosslinker, …
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QUANTIFYING OLIGOMERIC STATES OF PROTEINS IN-CELL VIA SINGLE-MOLECULE SUPER-RESOLUTION MICROSCOPY
… describes our efforts in developing in-cell single-molecule imaging assays to quantify oligomeric states of membrane and cytosolic proteins. Quantifying oligomeric states of highly-expressed membrane proteins were achieved using protein localizations obtained from super-resolution microscopy. …
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Characterization and cooperative dynamics of kinesin motor proteins in intracellular transport
… teams of motor proteins rather than by isolated molecules, yet the rules governing multi-motor coordination remain poorly understood because motor number, spacing, and composition are difficult to control experimentally. In this dissertation, I developed both the molecular engineering and …
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EFFECT OF MISMATCHED BASE PAIRS ON DNA PLECTONEMES
… have shown that in DNA harboring a single mismatch, the plectoneme will always localize at the mismatch. Theoretical studies have predicted that under physiological salt concentrations, plectoneme localization becomes probabilistic, i.e., the plectoneme does not always localize at …